I-TASSER results

I-TASSER results for job id Rv1006

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (567 residues)
MVLRSRKSTLGVVVCLALVLGGPLNGCSSSASHRGPLNAMGSPAIPSTAQEIPNPLRGQY
EDLMEPLFPQGNPAQQRYPPWPASYDASLRVSWRQLQPTDPRTLPPDAPDDRKYDFSVID
NALTRLADRGMRLTLRVYAYSSCCKASYPDGTNIAIPDWERAIASTNTSYPGPATDPSTG
VVQVVPNFNDSTYLNDFAQLLAALGRRYDGDERLSVFEFSGYGDFSENHVAYLRDTLGAP
GPGPDESVATLGYYSQFRDQNITTASIKQLIAANVSAFPHTQLVTSPANPEIVRELFADE
VTNKLAAPVGVRSDCLGVDAPLPAWAESSTSHYVQTKDPVVAALRQRLATAPVITEWCEL
PTGSSPRAYYEKGLRDVIRYHVSMTSSVNFPDQTATSPMDPALYLVWAQANAAAGYRYSV
EAQPGSQALAGKVATISVTWTNYGAAAATEKWVPGYRLVDSTGQVVRTLPAAVDLKTLVS
DQRGDRSSDQPTPASVAETVRVDLSGLPAGHYTLRAAIDWQQHKPNGSHVVNYPPMLLSR
DGRDDSGFYPVATLDIPRDAQTAVNAS

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MVLRSRKSTLGVVVCLALVLGGPLNGCSSSASHRGPLNAMGSPAIPSTAQEIPNPLRGQYEDLMEPLFPQGNPAQQRYPPWPASYDASLRVSWRQLQPTDPRTLPPDAPDDRKYDFSVIDNALTRLADRGMRLTLRVYAYSSCCKASYPDGTNIAIPDWERAIASTNTSYPGPATDPSTGVVQVVPNFNDSTYLNDFAQLLAALGRRYDGDERLSVFEFSGYGDFSENHVAYLRDTLGAPGPGPDESVATLGYYSQFRDQNITTASIKQLIAANVSAFPHTQLVTSPANPEIVRELFADEVTNKLAAPVGVRSDCLGVDAPLPAWAESSTSHYVQTKDPVVAALRQRLATAPVITEWCELPTGSSPRAYYEKGLRDVIRYHVSMTSSVNFPDQTATSPMDPALYLVWAQANAAAGYRYSVEAQPGSQALAGKVATISVTWTNYGAAAATEKWVPGYRLVDSTGQVVRTLPAAVDLKTLVSDQRGDRSSDQPTPASVAETVRVDLSGLPAGHYTLRAAIDWQQHKPNGSHVVNYPPMLLSRDGRDDSGFYPVATLDIPRDAQTAVNAS
Prediction	CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEHCCCCCHHHHHHCCCHHHHHHHCCCEEHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEHCCCCCCEEEEEEEEEEEEEHCCCCCCEEEEEEEHCCCCEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHCCCCCCCCEEEEEEEEHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEHCCCCCCCCCC
Conf.Score	976666546799999999999987478888888887776667666787555788777776554545678888656765678776568999985774888888777777667855789999999999999998999999758888888887665556578987656776667777778888776789999999999999999999999868997899997456668887778777654456666545566666677776655776888888887788778765775577755666655556677765685565566778778877777887654677788899988876556655555678888885456778999999999998554556776555667756899999999986877999667764556996799999999966555777865899999599988999967888644689987765555678887567898767789998589999985888888766666776455445688888875667999855666678899
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MVLRSRKSTLGVVVCLALVLGGPLNGCSSSASHRGPLNAMGSPAIPSTAQEIPNPLRGQYEDLMEPLFPQGNPAQQRYPPWPASYDASLRVSWRQLQPTDPRTLPPDAPDDRKYDFSVIDNALTRLADRGMRLTLRVYAYSSCCKASYPDGTNIAIPDWERAIASTNTSYPGPATDPSTGVVQVVPNFNDSTYLNDFAQLLAALGRRYDGDERLSVFEFSGYGDFSENHVAYLRDTLGAPGPGPDESVATLGYYSQFRDQNITTASIKQLIAANVSAFPHTQLVTSPANPEIVRELFADEVTNKLAAPVGVRSDCLGVDAPLPAWAESSTSHYVQTKDPVVAALRQRLATAPVITEWCELPTGSSPRAYYEKGLRDVIRYHVSMTSSVNFPDQTATSPMDPALYLVWAQANAAAGYRYSVEAQPGSQALAGKVATISVTWTNYGAAAATEKWVPGYRLVDSTGQVVRTLPAAVDLKTLVSDQRGDRSSDQPTPASVAETVRVDLSGLPAGHYTLRAAIDWQQHKPNGSHVVNYPPMLLSRDGRDDSGFYPVATLDIPRDAQTAVNAS
Prediction	412324320000000000000000001344334424343233431324254041001010210020112334224542352431100000010230114434433443454120104202500540273501000000012224544144542320031024333434414344223443312100203122025103500520164045542000000000000000001224432444334343222224224403433135412320030026104312010224324323342244133313310000000001343243134443322242343225303520420000000022144331341235103300410110010010332313341354124003301430000010442423442544403030303020000002613000001056343134243734033011245343434333435242414141740441301000000023455434422522102001514555110200302033324444648
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEHCCCCCHHHHHHCCCHHHHHHHCCCEEHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEHCCCCCCEEEEEEEEEEEEEHCCCCCCEEEEEEEHCCCCEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHCCCCCCCCEEEEEEEEHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEHCCCCCCCCCC
MVLRSRKSTLGVVVCLALVLGGPLNGCSSSASHRGPLNAMGSPAIPSTAQEIPNPLRGQYEDLMEPLFPQGNPAQQRYPPWPASYDASLRVSWRQLQPTDPRTLPPDAPDDRKYDFSVIDNALTRLADRGMRLTLRVYAYSSCCKASYPDGTNIAIPDWERAIASTNTSYPGPATDPSTGVVQVVPNFNDSTYLNDFAQLLAALGRRYDGDERLSVFEFSGYGDFSENHVAYLRDTLGAPGPGPDESVATLGYYSQFRDQNITTASIKQLIAANVSAFPHTQLVTSPANPEIVRELFADEVTNKLAAPVGVRSDCLGVDAPLPAWAESSTSHYVQTKDPVVAALRQRLATAPVITEWCELPTGSSPRAYYEKGLRDVIRYHVSMTSSVNFPDQTATSPMDPALYLVWAQANAAAGYRYSVEAQPGSQALAGKVATISVTWTNYGAAAATEKWVPGYRLVDSTGQVVRTLPAAVDLKTLVSDQRGDRSSDQPTPASVAETVRVDLSGLPAGHYTLRAAIDWQQHKPNGSHVVNYPPMLLSRDGRDDSGFYPVATLDIPRDAQTAVNAS

5ccuA

0.14

0.11

0.76

1.03

MUSTER

-----------------------------PATPITTLQADGTHLVDGYGRTVL--LHGVNVDKDAPYLPAGETLTPQDIDI--FNTVRLGTSFDALMP-----------QRGQIDLDRLTGVVDALTARGMHVLLDNHQDGLS-----KAWGGNGFPEWAIESRPREWEPNPGFPLYYLMPSLNAGWGNTHGALDHLGTALGALAERVEGKPGVMGIELLN-----EPWPGSRFLSCFPNGC------------PDFDRT--YQAAMQKLTDAVRAQNPTIPVYWEPNVTWNQMMPSNPPVTPALTTADVVFAPHDYCIPSQLAIYLGLPQALRGLCVPQQDLTWSNIDAITANVPTVITEFGDGDPTVLKNTLARADERFIGWQYWHFGAGNATDPFLGEVGRQLV---------------RTYPQATAGEPGRMIFD--------------------ADNGDFAYRFT--------------------PRAATRPTEIFVSDLHYPDG-YAVQVD-------GQVTSAPGARIVTVVADGSGP------VTVKINRPGSAGAEVP

5ccuA

0.13

0.10

0.76

1.00

dPPAS

-------------------------------------PATPITTLQADGTHLVDG-YGNVDKDAPYLPAGETLTPQDIDILVRFNTVRLGTSFDALMPQRGQIDEAY--------LDRLTGVVDALTARGMHVLLDNH-----QDGLSKAWGGNGFPEWAIESRPREWEPNPGFPLYYLMPSLNAGWGNTHGALDHLGTALGALAERVEGKPGVMGIELLN-----EPWPGSRFLSCFPNGC--------------PDFDRTYQAAMQKLTDAVRAQNPTIPVYWEPNVTWNQMMPSNLFAPPVTPALTTADVVFAPHDYCIPSQLAIYLGLPQALRGLCVPQQDLTWSNIDAITERANVPTGDGDPTVLKNTLARADERFIGWQYWHFGAGNATDPFLGEVGRQLVR---------------TYPQATAGEPGRMIFD---------ADNGDFAYRFTPRAATRPTEIFVSDLHYPDGYAVQVDGGQVTSAPGARIVTVVADGSGP------VTVKINRPGSAGAEVP--------------------------------------

5ccuA

0.14

0.11

0.74

1.46

wdPPAS

-------------------------------------PATPITTLQADGTHLVDGLHGNVDKDAPYLPAGETLTPQDIDILVRFNTVRLGTSFDALMP-----------QRGQIDLDRLTGVVDALTARGMHVLLDNH-----QDGLSKAWGGNGFPEWAIESRPREWEPNPGFPLMPSLNAGWDEVWGNTHALDHLGTALGALAERVEGKPGVMGIEL-----LNE------------PWPGSRFLSCFPNGCPDFDRTY--QAAMQKLTDAVRAQNPTIPVYWEPNVTWNQMMPSNLVTPALTTADVVFAPHDYAIYLGLPQALRGLCVPQQDLTWSNIDAITERANVPTVITEFGDGDPT-----VLKNTLARADERFIGWQYWHFGAGNATDPFLGEVGRQLVR---------------TYPQATAGEPGRMIFD--------------------ADNGDFAYRFTPR--------------------AATRPTEIFVSDLHYPDG-YAVQVD--------QVTSAPGARIVTVVADGSGP------VTVKINRPGSAGAEVP

5ccuA

0.15

0.11

0.75

1.15

wMUSTER

------------------------------ATPITTLQADGTHLVDGYGRTVL--LHGVNVDKDAPYLPAGETLTPQDIDI--FNTVRLGTSFDALMP-----------QRGQIDLDRLTGVVDALTARGMHVLLDNHQDGLSKA-----WGGNGFPEWAIESRPREPNPGFPLYYLMPSLNAGWDEVNTHGALDHLGTALGALAERVEGKPGVMGIEL-----LNEPWPGSRFLSCFPNGC------------PDFDRTY--QAAMQKLTDAVRAQNPTIPVYWEPNVTWNQMMPSNLVTPALTTADVVFAPHDYCIPSQLAIYLGLPQVPQQDLTWSNIDAITERANVPTVITEFGDGDPT-----VLKNTLARADERFIGWQYWHFGAGNATDPFLGEVGRQLV------RTYPQATAGEPGRMIFDADNGDFAYRFTPRA-----ATRPTEIFV---------------------S--------DLHYPDGYAVQVD--------GQVT---------------SAPGARIVTVVADGSGP------VTVKINRPGSAGAEVP

5ccuA

0.15

0.11

0.69

1.67

wPPAS

------------------------------------------------------PLHGVNVDKDAPYLPAGETLTPQD-----FNTVRLGTSFDALMP-----------QRGQIDLDRLTGVVDALTARGMHVLLDN------QDGLSKAWGGNGFPEWAIESRPREWEPNPGFPLYYLMNAGWDEVWGNTHALDHLGTALGALAERVEGKPGVMGIEL-----LNEPWPGSRFLSCFPNGC------------PDFDRTY--QAAMQKLTDAVRAQNPTIPVYWEPNVTWNQMMPSNLVTPALTTADVVFAPHDYCIPSQLAGLPQALRGLCVPQQDLTWSNIDAITENVPVITEFGDGDPT-----VLKNTLARADERFIGWQYWHFGAGNATDPFLGEVGRQLVR---------------TYPQATAGEPGRMIFD--------------------ADNGDFAYRFTPRAATRPFVSDLH-----------------------YPDG-YAVQVD--------QVTSAPGARIVTVVADGSGP------VTVKINRPGSAGAEVP

5ccuA

0.13

0.10

0.74

2.00

dPPAS2

-------------------------------------PATPITTLQADGTHLVDG-YGNVDKDAPYLPAGETLTPQDIDILVRFNTVRLGTSFDALMPQRGQIDEAY--------LDRLTGVVDALTARGMHVLLDNH-----QDGLSKAWGGNGFPEWAIESRPREWEPNPGFPLYYLMPSLNAGWGNTHGALDHLGTALGALAERVEGKPGVMGIELLN-----EPWPGSRFLSCFPNGC--------------PDFDRTYQAAMQKLTDAVRAQNPTIPVYWEPNVTWNQMMPSNLFAPPVTPALTTADVVFAPHDYCIPSQLAIYLGLPQALRGLCVPQQDLTWSNIDAITERANVPTGDGDPTVLKNTLARADERFIGWQYWHFGAGNATDPFLGEVGRQLVR---------------TYPQATAGEPGRMIFD--------------------ADNGDFAYRFTPRAATRPFVSDLHYPDGYATSAPGARIVTVVADGSG------PVTVKINRPGSAGAEVP--------------------------------------

5ccuA

0.15

0.11

0.71

1.51

PPAS

------------------------------------------------------PATPITTDKDAPYLPAGETLTPQD-----FNTVRLGTSFDALMP-----------QRGQIDLDRLTGVVDALTARGMHVLLDN------QDGLSKAWGGNGFPEWAIESRPREWEPNPGFPLYYLMPSLNAG-GNTHGALDHLGTALGALAERVEGKPGVMGIEL-----LNEPWPGSRFLSCFPNGC--------------PDFDRTYQAAMQKLTDAVRAQNPTIPVYWEPN------------VTWNQMMPSNLFAPPVTPALTTADVVFAPHDYCIPSQLAIYLGLPQALR------------------GLCVPQQDLTWSNIDAITERANVPTVITGDGDPTVLKNTLARADERIGWQYWH---FGAGNATDPFLGEVGRQLVRTYPQATAGEPGRMIFDADNGDFAYRFTPR--------------------AATRPTEIFVSDLHYPDG-YAVQVD-------GQVTSAPGARIVTVVADGSGP------VTVKINRPGSAGAEVP

1kwgA1

0.16

0.05

0.28

1.74

Env-PPAS

-------------------------------------------------------------------------------------VRIGEFAWALLEP-----------EPGRLEWGWLDEAIATLAAEGLKVVLGTP--------------TATPPKWLVDRYPEILPVDREGRRRRFGGRRHY-CFSSPVYREEARRIVTLLAERYGGLEAVAGFQT-----DNECHDTVVRAFNRLQVEILRAHAPGKFVTHNFMGFFTD---LDAFALAQDLDFAS-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

2y8kA

0.11

0.09

0.83

0.98

MUSTER

--------------------------ASSPQRGRPRLNAARTTFVGDN----GQPLRGPYTS----TEWTAAAPYDQIARVKELGFNAVHLYAECFDPRYPAPGSKAP----GYAVNEIDKIVERTRELGLYLVITIGN-----GANNGNHNAQWARDFWKFYAPRYAKETHHNEPVAWGPPYSSSTANPPGAVDMEIDVYRIIRTYAPETP-VLLFSYAVFGGKGGAAEALKDIRAFNKAVFGNENAVWTNEAVAFHGYAGWQETTIAVEELLKAGYP-CFMTEYAGGGSGMGGLDVELTYELERLGVSWLTFQY-IPPTGVSDDVTKPEYFSALVENSGLSWTPDYGNWPAARGVYGNGGLARETATWINNFLT-GTTRIEAEDFDWGGNGVSYYDTDSVNVG--GQYRPDEGVDIEKGGYNVGWISEGEWLEYTIRVRNPGYY------NLSLRVAGISGSRVQVSFGNQDK-------TGVWELPATGGFQTWTTATRQVF-LGAGLQKLRINALS--------------------------GGFNLNWIELSPI--------

2osxA

0.11

0.08

0.75

0.90

dPPAS

---------------------------------------TPSYLKDDDGRSLILNTASSAKSAPDGMPQFTEADLAREYADMGTNFVRFLISWRSVEPAPGVYDQQY--------LDRVEDRVGWYAERGYKVMLDMHQDVYSGAITPEGAIGNGAPAWATYMDGLPVEPQPRWELYYIQDNFWNTTGKHPELVEHYAKAWRAVADRFADNDAVVAYDLMN-----EPFGGSLQG--------------------PAFEAGPLAAMYQRTTDAIRQVDQDTWVCVAPQAIGVNQGLPSGLTKIDDPRAPHLYPLPLDIGDGHEGLARTLTDVTIDAWRANTAHTARVLGDVPILGSFGLDTTLPGARDYIERVYGTAREMGAGVSYWSSDPGPWGPYLPDGTQTLLVDTLNK-----------PYPRAVAGTPTEWSSTSD-----------RLQLTIEPDAAITAPTEIY---LPEAGFPGDVHVEGADVVGWDRQSRLLTVRTPADSGNVTVTVTPAA-----------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-2.96

Estimated TM-score = 0.38±0.13

Estimated RMSD = 15.0±3.5Å

Download Model 2

C-score=-3.42

Download Model 3

C-score=-3.81

Download Model 4

C-score=-3.77

Download Model 5

C-score=-3.93

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	5ccuA	0.741	1.64	0.129	0.767
2	2osxA	0.651	3.24	0.100	0.727
3	4d1iA	0.548	5.40	0.101	0.697
4	3u7vA	0.534	5.28	0.117	0.681
5	3og2A	0.529	5.22	0.096	0.670
6	1tg7A	0.526	5.30	0.067	0.674
7	3icgA	0.519	4.87	0.088	0.638
8	4uzsA	0.513	5.22	0.101	0.651
9	4uniA	0.511	5.26	0.101	0.651
10	1uz4A	0.510	4.00	0.059	0.593

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.10	6	2osyB	GLC	Rep, Mult	62,138,140,174,226,317,361,387
2	0.07	4	1wdqA	MAL	Rep, Mult	91,93,137,227,286,314,360,361,362,387
3	0.03	2	1nw1A	CA	Rep, Mult	212,227,230,232
4	0.03	2	2cksA	ZN	Rep, Mult	227,356
5	0.03	2	5j14A	GAL	Rep, Mult	62,174,326,387
6	0.02	1	3ndzA	CT3	Rep, Mult	117,118,121
7	0.02	1	3k7tA	GP7	Rep, Mult	240,241
8	0.02	1	2akfB	ZN	Rep, Mult	209,212
9	0.02	1	2oymA	MNI	Rep, Mult	62,138,140,175,178,226,227,229,290,317,361,387
10	0.02	1	2o011	CLA	Rep, Mult	223,224
11	0.02	1	2zumA	ZN	Rep, Mult	54,424
12	0.02	1	1b3zA	XYP	Rep, Mult	207,208,213
13	0.02	1	1j0zB	CA	Rep, Mult	97,111,112,159,196,199
14	0.02	1	1b1yA	UUU	Rep, Mult	92,136,286
15	0.02	1	3c5iA	MG	Rep, Mult	303,314
16	0.02	1	2h8pC	GOA	Rep, Mult	415,416
17	0.02	1	2xgbA	EPG	Rep, Mult	90,92,388
18	0.02	1	1h5vA	CA	Rep, Mult	54,381

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.254	2osxA	0.651	3.24	0.100	0.727	3.2.1.123	NA
2	0.112	2oykB	0.645	3.21	0.100	0.720	3.2.1.123	NA
3	0.060	1b1yA	0.500	5.00	0.074	0.624	3.2.1.2	160
4	0.060	1d7fA	0.470	5.91	0.053	0.633	2.4.1.19	NA
5	0.060	1qhoA	0.468	5.93	0.058	0.633	3.2.1.133	NA
6	0.060	1m53A	0.483	6.32	0.070	0.675	5.4.99.12	218
7	0.060	1izjA	0.473	6.52	0.044	0.675	3.2.1.135,3.2.1.1	NA
8	0.060	1cgtA	0.469	5.88	0.051	0.633	2.4.1.19	225
9	0.060	1eceB	0.466	3.78	0.108	0.536	3.2.1.4	318
10	0.060	1qnoA	0.469	3.60	0.065	0.536	3.2.1.78	227,359
11	0.060	3a47A	0.495	6.30	0.048	0.690	3.2.1.10	134,215
12	0.060	3icgA	0.519	4.87	0.088	0.638	3.2.1.4	285
13	0.060	1rh9A	0.481	3.68	0.088	0.550	3.2.1.78	359
14	0.060	1cygA	0.473	5.86	0.051	0.637	2.4.1.19	NA
15	0.060	1cdgA	0.472	5.82	0.060	0.631	2.4.1.19	NA
16	0.060	1b90A	0.511	5.58	0.089	0.668	3.2.1.2	NA
17	0.060	1wdpA	0.495	5.00	0.076	0.619	3.2.1.2	NA
18	0.060	2fhbA	0.483	6.10	0.070	0.663	3.2.1.41	62,93
19	0.060	1fa2A	0.500	5.16	0.067	0.630	3.2.1.2	NA
20	0.060	3k1dA	0.473	6.68	0.068	0.682	2.4.1.18	NA
21	0.060	2zidA	0.476	6.17	0.075	0.656	3.2.1.70	218

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.22	0.651	3.24	0.10	0.73	2osxA	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0047876
1	0.13	0.741	1.65	0.13	0.77	5j14A	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798
2	0.11	0.465	4.01	0.10	0.54	3ndyA	GO:0000272 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0030246 GO:0030247
3	0.07	0.546	5.15	0.11	0.69	3u7vA	GO:0004553 GO:0004565 GO:0005975 GO:0008152 GO:0009341 GO:0016787 GO:0016798
4	0.07	0.487	4.14	0.09	0.57	4yheA	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798
5	0.07	0.485	3.79	0.07	0.56	4w8aA	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798
6	0.07	0.476	3.91	0.09	0.55	3ayrA	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798
7	0.07	0.471	3.79	0.07	0.54	1edgA	GO:0000272 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245
8	0.07	0.466	3.77	0.11	0.54	1eceA	GO:0000272 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0030246 GO:0030247
9	0.07	0.472	3.94	0.10	0.55	4nf7A	GO:0000272 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0030246 GO:0030247
10	0.07	0.464	3.65	0.08	0.53	2jepA	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0033946
11	0.07	0.463	3.94	0.08	0.54	4x0vC	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798
12	0.07	0.464	3.77	0.07	0.54	5d9nA	GO:0004553 GO:0005975 GO:0006080 GO:0016985
13	0.07	0.454	3.70	0.08	0.52	4tufA	GO:0000272 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0030246 GO:0030247
14	0.07	0.460	3.73	0.10	0.53	4w84A	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0033946
15	0.07	0.456	3.86	0.08	0.53	4im4A	GO:0000272 GO:0003824 GO:0004553 GO:0005576 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0030248 GO:0045493 GO:0046555 GO:0046872 GO:2000884
16	0.07	0.453	3.72	0.11	0.52	4lx4B	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798
17	0.06	0.452	3.48	0.10	0.51	4htyA	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798
18	0.06	0.451	3.26	0.07	0.51	5bywE	GO:0000272 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245

Consensus prediction of GO terms

Molecular Function	GO:0004553
GO-Score	0.47
Biological Processes	GO:0005975
GO-Score	0.47
Cellular Component	GO:0009341
GO-Score	0.07

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.