I-TASSER results

I-TASSER results for job id Rv1003

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (285 residues)
MSSGRLLLGATPLGQPSDASPRLAAALATADVVAAEDTRRVRKLAKALDIRIGGRVVSLF
DRVEALRVTALLDAINNGATVLVVSDAGTPVISDPGYRLVAACIDAGVSVTCLPGPSAVT
TALVMSGLPAEKFCFEGFAPRKGAARRAWLAELAEERRTCVFFESPRRLAACLNDAVEQL
GGARPAAICRELTKVHEEVVRGSLDELAIWAAGGVLGEITVVVAGAAPHAELSSLIAQVE
EFVAAGIRVKDACSEVAAAHPGVRTRQLYDAVLQSRRETGGPAQP

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSSGRLLLGATPLGQPSDASPRLAAALATADVVAAEDTRRVRKLAKALDIRIGGRVVSLFDRVEALRVTALLDAINNGATVLVVSDAGTPVISDPGYRLVAACIDAGVSVTCLPGPSAVTTALVMSGLPAEKFCFEGFAPRKGAARRAWLAELAEERRTCVFFESPRRLAACLNDAVEQLGGARPAAICRELTKVHEEVVRGSLDELAIWAAGGVLGEITVVVAGAAPHAELSSLIAQVEEFVAAGIRVKDACSEVAAAHPGVRTRQLYDAVLQSRRETGGPAQP
Prediction	CCCCEEEEEHCCCCCHHHHCHHHHHHHHHCCEEEHCCCCHHHHHHHHHCCCCCCCEEHCCHHHHHHHHHHHHHHHHHCCCEEEEHCCCCCHCCCCHHHHHHHHHHHCCCEEHCCCHHHHHHHHHHHCCCCCCEEEEHCCCCCHHHHHHHHHHHHHCCCCEEEEHCHHHHHHHHHHHHHHHCCCCEEEEEHCCCCCCEEEEHCCHHHHHHHHHCCCCCEEEEEHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
Conf.Score	999879999589999677559999999979899986885799999998998888656635667899999999999989969999589998557976999999999899799968868999999996999998899875899958999999999978996999967588999999999998999768887536887658997599999999975899779999858998889689999999999989999999999999996999999999999999665788899
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSSGRLLLGATPLGQPSDASPRLAAALATADVVAAEDTRRVRKLAKALDIRIGGRVVSLFDRVEALRVTALLDAINNGATVLVVSDAGTPVISDPGYRLVAACIDAGVSVTCLPGPSAVTTALVMSGLPAEKFCFEGFAPRKGAARRAWLAELAEERRTCVFFESPRRLAACLNDAVEQLGGARPAAICRELTKVHEEVVRGSLDELAIWAAGGVLGEITVVVAGAAPHAELSSLIAQVEEFVAAGIRVKDACSEVAAAHPGVRTRQLYDAVLQSRRETGGPAQP
Prediction	674210000002012253023102400440100000023203300430406343420212443455305500520463430000030200000210130032027470402000110000000000013032000010224555413511540472710000011222034004201730374010000120024303212030530351377501000000012356555265015303510677152350023005624714144005202621677856768
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCEEEEEHCCCCCHHHHCHHHHHHHHHCCEEEHCCCCHHHHHHHHHCCCCCCCEEHCCHHHHHHHHHHHHHHHHHCCCEEEEHCCCCCHCCCCHHHHHHHHHHHCCCEEHCCCHHHHHHHHHHHCCCCCCEEEEHCCCCCHHHHHHHHHHHHHCCCCEEEEHCHHHHHHHHHHHHHHHCCCCEEEEEHCCCCCCEEEEHCCHHHHHHHHHCCCCCEEEEEHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
MSSGRLLLGATPLGQPSDASPRLAAALATADVVAAEDTRRVRKLAKALDIRIGGRVVSLFDRVEALRVTALLDAINNGATVLVVSDAGTPVISDPGYRLVAACIDAGVSVTCLPGPSAVTTALVMSGLPAEKFCFEGFAPRKGAARRAWLAELAEERRTCVFFESPRRLAACLNDAVEQLGGARPAAICRELTKVHEEVVRGSLDELAIWAAGGVLGEITVVVAGAAPHAELSSLIAQVEEFVAAGIRVKDACSEVAAAHPGVRTRQLYDAVLQSRRETGGPAQP

3kwpA

0.44

0.34

0.79

2.47

MUSTER

-TGGHLYLVPTPIGNLDD-TFRAVKTLTAVDLIAAEDTRNTQKLLNHFEITTK--QISFHEHNTQERIPQLIAKLKQG-QIAQVSDAG-PSISDPGHELVNACIDAHIPVVPLPGANAGLTALIASGLAPQPFYFYGFLDRKPKDRKAEIAGLAQRPETLIFYEAPHRLKKTLQNLAAGFGDERPAVLCRELTKRYEEFLRGSLAELANWAATTVRGEFVVLVGGNPAPT-------------------------------------------------------

3kwpA

0.44

0.34

0.79

3.16

dPPAS

-TGGHLYLVPTPIGNLDD-TFRAVKTLTAVDLIAAEDTRNTQKLLNHFEITTK--QISFHEHNTQERIPQLIAKLKQG-QIAQVSDAG-PSISDPGHELVNACIDAHIPVVPLPGANAGLTALIASGLAPQPFYFYGFLDRKPKDRKAEIAGLAQRPETLIFYEAPHRLKKTLQNLAAGFGDERPAVLCRELTKRYEEFLRGSLAELANWAATTVRGEFVVLVGGNPAPT-------------------------------------------------------

3kwpA

0.44

0.34

0.79

3.93

wdPPAS

-TGGHLYLVPTPIGNLDD-TFRAVKTLTAVDLIAAEDTRNTQKLLNHFEITTK--QISFHEHNTQERIPQLIAKLKQG-QIAQVSDAG-PSISDPGHELVNACIDAHIPVVPLPGANAGLTALIASGLAPQPFYFYGFLDRKPKDRKAEIAGLAQRPETLIFYEAPHRLKKTLQNLAAGFGDERPAVLCRELTKRYEEFLRGSLAELANWAATTVRGEFVVLVGGNPAPT-------------------------------------------------------

3kwpA

0.44

0.34

0.78

3.22

wMUSTER

--GGHLYLVPTPIGNLDD-TFRAVKTLTAVDLIAAEDTRNTQKLLNHFEITTK--QISFHEHNTQERIPQLIAKLKQG-QIAQVSDAG-PSISDPGHELVNACIDAHIPVVPLPGANAGLTALIASGLAPQPFYFYGFLDRKPKDRKAEIAGLAQRPETLIFYEAPHRLKKTLQNLAAGFGDERPAVLCRELTKRYEEFLRGSLAELANWAATTVRGEFVVLVGGNPAPT-------------------------------------------------------

3kwpA

0.44

0.34

0.79

4.03

wPPAS

-TGGHLYLVPTPIGNLDD-TFRAVKTLTAVDLIAAEDTRNTQKLLNHFEITTK--QISFHEHNTQERIPQLIAKLKQG-QIAQVSDAG-PSISDPGHELVNACIDAHIPVVPLPGANAGLTALIASGLAPQPFYFYGFLDRKPKDRKAEIAGLAQRPETLIFYEAPHRLKKTLQNLAAGFGDERPAVLCRELTKRYEEFLRGSLAELANWAATTVRGEFVVLVGGNPAPT-------------------------------------------------------

3kwpA

0.44

0.34

0.79

5.70

dPPAS2

-TGGHLYLVPTPIGNLDD-TFRAVKTLTAVDLIAAEDTRNTQKLLNHFEITTK--QISFHEHNTQERIPQLIAKLKQG-QIAQVSDAG-PSISDPGHELVNACIDAHIPVVPLPGANAGLTALIASGLAPQPFYFYGFLDRKPKDRKAEIAGLAQRPETLIFYEAPHRLKKTLQNLAAGFGDERPAVLCRELTKRYEEFLRGSLAELANWAATTVRGEFVVLVGGNPAPT-------------------------------------------------------

3kwpA

0.44

0.34

0.79

3.60

PPAS

-TGGHLYLVPTPIGNLDD-TFRAVKTLTAVDLIAAEDTRNTQKLLNHFEITTK--QISFHEHNTQERIPQLIAKLKQG-QIAQVSDAG-PSISDPGHELVNACIDAHIPVVPLPGANAGLTALIASGLAPQPFYFYGFLDRKPKDRKAEIAGLAQRPETLIFYEAPHRLKKTLQNLAAGFGDERPAVLCRELTKRYEEFLRGSLAELANWAATTVRGEFVVLVGGNPAPT-------------------------------------------------------

3kwpA

0.44

0.34

0.79

6.00

Env-PPAS

-TGGHLYLVPTPIGNLDD-TFRAVKTLTAVDLIAAEDTRNTQKLLNHFEITT--KQISFHEHNTQERIPQLIAKLKQG-QIAQVSDAG-PSISDPGHELVNACIDAHIPVVPLPGANAGLTALIASGLAPQPFYFYGFLDRKPKDRKAEIAGLAQRPETLIFYEAPHRLKKTLQNLAAGFGDERPAVLCRELTKRYEEFLRGSLAELANWAATDVRGEFVVLVGGNPAPT-------------------------------------------------------

1wyzB

0.18

0.14

0.76

2.14

MUSTER

--ETALYLLPVTLGDEQVLPSYNTEIIRGIRHFIVEDVRSARRFLKKVDREIDIDSLTFYPLNKHTSPEDISGYLK-----PLAGGASGVISEDPGADVVAIAQRQKLKVIPLVGPSSIILSVA-SGFNGQSFAFHGYLPIEPGERAKKLKTLEQRSQTQLFIETPYRNHK-IEDILQNCRPQTKLCIAANITCEGEFIQTRTVKDWKGHIPELSKIPCIFLLYKL-----------------------------------------------------------

1wyzB

0.18

0.14

0.76

2.68

dPPAS

--ETALYLLPVTLGDEQVLPSYNTEIIRGIRHFIVEDVRSARRFLKKVDREIDIDSLTFYPLNKHTSPEDISGYLK-----PLAGGASGVISEDPGADVVAIAQRQKLKVIPLVGPSSIILSVA-SGFNGQSFAFHGYLPIEPGERAKKLKTLEQRSQTQLFIETPYRNH-KIEDILQNCRPQTKLCIAANITCEGEFIQTRTVKDWKGHIPELSKIPCIFLLYKL-----------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.03

Estimated TM-score = 0.58±0.14

Estimated RMSD = 8.4±4.5Å

Download Model 2

C-score=-1.88

Download Model 3

C-score=-2.06

Download Model 4

C-score=-2.19

Download Model 5

C-score=-2.82

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3kwpA	0.756	1.27	0.438	0.786
2	1wyzB	0.654	2.65	0.206	0.751
3	1wdeA	0.653	3.58	0.191	0.807
4	2dv7A	0.620	3.25	0.122	0.751
5	1vhvB	0.614	3.21	0.137	0.740
6	3i4tA	0.601	3.33	0.094	0.733
7	1cbfA	0.599	3.86	0.183	0.775
8	1s4dE	0.593	3.81	0.158	0.775
9	2zvcA	0.587	3.80	0.165	0.765
10	1pjsB	0.586	3.69	0.175	0.754

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.61	30	5hw4A	SAM	Rep, Mult	13,87,88,89,92,93,117,118,138,163,189,191,192,218,219,220

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.193	1vhvB	0.614	3.21	0.137	0.740	2.1.1.98	13,18,20,88,91,115,117,163
2	0.180	1wdeA	0.653	3.58	0.191	0.807	2.1.1.98	8,87,91,115
3	0.157	1vceA	0.621	3.31	0.113	0.754	2.1.1.98	NA
4	0.075	2e0kB	0.572	3.77	0.140	0.740	2.1.1.130	115
5	0.072	1ve2A	0.567	3.38	0.185	0.705	2.1.1.107	37
6	0.067	1s4dE	0.593	3.81	0.158	0.775	2.1.1.107	NA
7	0.060	3bfjA	0.444	5.11	0.071	0.642	1.1.1.202	NA
8	0.060	2hrbA	0.438	5.40	0.086	0.677	1.1.1.184	97
9	0.060	1v9aB	0.557	3.80	0.143	0.719	2.1.1.107	NA
10	0.060	3igyB	0.447	5.43	0.091	0.684	5.4.2.1	190
11	0.060	1o0sA	0.454	5.52	0.072	0.702	1.1.1.38	NA
12	0.060	1gz3A	0.453	5.43	0.064	0.698	1.1.1.38	NA
13	0.060	2esdA	0.447	5.51	0.075	0.695	1.2.1.9	20
14	0.060	2a9fA	0.441	5.69	0.052	0.681	1.1.1.39	NA
15	0.060	1gq2A	0.457	5.39	0.057	0.695	1.1.1.40	NA
16	0.060	3bfjT	0.440	5.19	0.071	0.646	1.1.1.202	NA
17	0.060	1q16A	0.356	6.58	0.038	0.628	1.7.99.4	NA
18	0.060	3c39A	0.444	5.94	0.072	0.716	2.7.2.3	NA
19	0.060	2cunA	0.462	5.85	0.060	0.726	2.7.2.3	NA
20	0.060	2z6rB	0.617	3.30	0.118	0.751	2.1.1.98	13,18,88
21	0.060	2p9tA	0.443	5.78	0.073	0.709	2.7.2.3	NA
22	0.060	2dvmA	0.464	5.26	0.047	0.698	1.1.1.38	NA
23	0.060	1wmaA	0.443	5.31	0.106	0.674	1.1.1.184	NA
24	0.060	13pkA	0.462	5.90	0.080	0.740	2.7.2.3	NA
25	0.060	1n5dA	0.442	5.48	0.115	0.681	1.1.1.184,1.1.1.189,1.1.1.197	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.51	0.765	1.35	0.43	0.80	3kwpA	GO:0000453 GO:0005737 GO:0006364 GO:0008152 GO:0008168 GO:0016740 GO:0032259 GO:0070677
1	0.34	0.372	2.05	0.50	0.40	3fq6B	GO:0000453 GO:0005737 GO:0006364 GO:0008152 GO:0008168 GO:0016740 GO:0032259 GO:0070677
2	0.31	0.664	2.64	0.20	0.76	1wyzB	GO:0008152 GO:0008168 GO:0016740 GO:0032259
3	0.29	0.351	2.34	0.37	0.39	3hh1A	GO:0000453 GO:0005737 GO:0006364 GO:0008152 GO:0008168 GO:0016740 GO:0032259 GO:0070677
4	0.27	0.361	1.86	0.48	0.39	3ffyA	GO:0000453 GO:0005737 GO:0006364 GO:0008152 GO:0008168 GO:0016740 GO:0032259 GO:0070677
5	0.19	0.601	3.64	0.19	0.76	3nutC	GO:0008152 GO:0008168 GO:0009236 GO:0016740 GO:0032259
6	0.17	0.599	3.86	0.18	0.78	1cbfA	GO:0008152 GO:0008168 GO:0009236 GO:0016740 GO:0032259 GO:0046026 GO:0055114
7	0.14	0.582	3.25	0.15	0.72	2qbuA	GO:0008152 GO:0008168 GO:0008757 GO:0009236 GO:0016740 GO:0030788 GO:0032259
8	0.12	0.577	3.97	0.17	0.76	2yboA	GO:0004851 GO:0006779 GO:0008152 GO:0008168 GO:0016740 GO:0032259 GO:0055114
9	0.11	0.567	3.38	0.18	0.71	1ve2A	GO:0006779 GO:0008152 GO:0008168 GO:0016740 GO:0032259 GO:0055114
10	0.10	0.659	3.66	0.18	0.82	1wdeA	GO:0004164 GO:0008152 GO:0008168 GO:0016740 GO:0017183 GO:0032259
11	0.07	0.622	3.23	0.12	0.75	2dekA	GO:0004164 GO:0008152 GO:0008168 GO:0016740 GO:0017183 GO:0032259 GO:0050843
12	0.07	0.626	3.11	0.13	0.75	1vhvB	GO:0004164 GO:0008152 GO:0008168 GO:0016740 GO:0017183 GO:0032259
13	0.07	0.601	3.33	0.09	0.73	3i4tA	GO:0004164 GO:0008152 GO:0008168 GO:0017183 GO:0032259
14	0.07	0.575	3.81	0.12	0.75	4e16A	GO:0008152 GO:0008168 GO:0009236 GO:0016740 GO:0032259 GO:0046026 GO:0055114
15	0.06	0.372	6.40	0.06	0.65	4fb5A	GO:0008152 GO:0016491 GO:0055114
16	0.06	0.312	6.21	0.05	0.53	1o68B	GO:0003824 GO:0003864 GO:0005737 GO:0015940 GO:0016740 GO:0046872
17	0.06	0.270	6.47	0.03	0.45	3q0qA	GO:0003723 GO:0003730 GO:0005737 GO:0006417 GO:0010494 GO:0010608 GO:0031965 GO:0034063 GO:0043488 GO:0044822 GO:0048471 GO:0051983 GO:0060964 GO:1900246 GO:2000637
18	0.06	0.288	5.80	0.05	0.46	1o66E	GO:0003824 GO:0003864 GO:0005737 GO:0015940 GO:0016740 GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0070677
GO-Score	0.83
Biological Processes	GO:0000453
GO-Score	0.83
Cellular Component	GO:0005737
GO-Score	0.83

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.