I-TASSER results

I-TASSER results for job id Rv0999

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (252 residues)
MRPPLAPQFAADLLVKTVSTLRSSGAALGRLTTMRKAVLAVGSVCWLVGCSSGASSTTAS
TGDIAKVAEVKSGFGPEYTVTDVTPRAIDPGFFSARKLPDGLSFDPANCAQVAAGPQLPT
GLQGNMAAVSAEGNGNRFVVIAVETSQPLPAPSPGKDCSKVTFSGTQLRGGIEVVDVPHI
DGTQTLGVHRVLQAVVGGSARTGELYDYSARFGDYQVIVIANPLVIPGRPVARVDTQRAR
DLLVQAVAAVRG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MRPPLAPQFAADLLVKTVSTLRSSGAALGRLTTMRKAVLAVGSVCWLVGCSSGASSTTASTGDIAKVAEVKSGFGPEYTVTDVTPRAIDPGFFSARKLPDGLSFDPANCAQVAAGPQLPTGLQGNMAAVSAEGNGNRFVVIAVETSQPLPAPSPGKDCSKVTFSGTQLRGGIEVVDVPHIDGTQTLGVHRVLQAVVGGSARTGELYDYSARFGDYQVIVIANPLVIPGRPVARVDTQRARDLLVQAVAAVRG
Prediction	CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEHCCCCCCCCCCCCCCCCCCCCEHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHCCCEEEEEEEHCCCCCCCCCCHHHCCEEEEEHCCCEEEEEEHCCCCCCCCEEEEEEEEEEEHCCCCCEEEEEEEEEEEHCCEEEEEEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
Conf.Score	998886567788877767654468875444344456788887765534689999999988898778887777589996787678877886454467899996886886555567877888877657899998589679999998689988887655487799995885789999779988876467777788874788655566888999998889999986445788888889999999999999999769
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MRPPLAPQFAADLLVKTVSTLRSSGAALGRLTTMRKAVLAVGSVCWLVGCSSGASSTTASTGDIAKVAEVKSGFGPEYTVTDVTPRAIDPGFFSARKLPDGLSFDPANCAQVAAGPQLPTGLQGNMAAVSAEGNGNRFVVIAVETSQPLPAPSPGKDCSKVTFSGTQLRGGIEVVDVPHIDGTQTLGVHRVLQAVVGGSARTGELYDYSARFGDYQVIVIANPLVIPGRPVARVDTQRARDLLVQAVAAVRG
Prediction	853522441002001410430356443333233032000010010000011364655444513143045046623761524634554133421343412651514255036315354344445242313324373210000002155514265437504402031441414044261261641423112321433364454444312000214421010002211336443451446203400250155158
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEHCCCCCCCCCCCCCCCCCCCCEHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHCCCEEEEEEEHCCCCCCCCCCHHHCCEEEEEHCCCEEEEEEHCCCCCCCCEEEEEEEEEEEHCCCCCEEEEEEEEEEEHCCEEEEEEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
MRPPLAPQFAADLLVKTVSTLRSSGAALGRLTTMRKAVLAVGSVCWLVGCSSGASSTTASTGDIAKVAEVKSGFGPEYTVTDVTPRAIDPGFFSARKLPDGLSFDPANCAQVAAGPQLPTGLQGNMAAVSAEGNGNRFVVIAVETSQPLPAPSPGKDCSKVTFSGTQLRGGIEVVDVPHIDGTQTLGVHRVLQAVVGGSARTGELYDYSARFGDYQVIVIANPLVIPGRPVARVDTQRARDLLVQAVAAVRG

4tmdA

0.57

0.43

0.75

2.26

MUSTER

---------------------------------------------------------AYPDADIAKVAQLKSSFGPEFKVSEVAPTGIDPKLLSPQKLPEGVKFEPADCAKFAEGQQFPPGLQGNMAATAAEGEGNRFIVMAVETSEPVPLSDPGDECKRVKFLGTGARGQVDVVESPQIDDARTVGTHRIIQTMR-----TGELYNYVASFDNYMVIVTANPLVLPDKPVAKVDTERARELLSAAVAAVRA

4tmdA

0.57

0.43

0.75

3.11

dPPAS

---------------------------------------------------------AYPDADIAKVAQLKSSFGPEFKVSEVAPTGIDPKLLSPQKLPEGVKFEPADCAKFAEGQQFPPGLQGNMAATAAEGEGNRFIVMAVETSEPVPLSDPGDECKRVKFLGTGARGQVDVVESPQIDDARTVGTHRIIQTMR-----TGELYNYVASFDNYMVIVTANPLVLPDKPVAKVDTERARELLSAAVAAVRA

4tmdA

0.57

0.43

0.75

4.92

wdPPAS

---------------------------------------------------------AYPDADIAKVAQLKSSFGPEFKVSEVAPTGIDPKLLSPQKLPEGVKFEPADCAKFAEGQQFPPGLQGNMAATAAEGEGNRFIVMAVETSEPVPLSDPGDECKRVKFLGTGARGQVDVVESPQIDDARTVGTHRIIQTMR-----TGELYNYVASFDNYMVIVTANPLVLPDKPVAKVDTERARELLSAAVAAVRA

4tmdA

0.58

0.43

0.75

2.73

wMUSTER

----------------------------------------------------------YPDADIAKVAQLKSSFGPEFKVSEVAPTGIDPKLLSPQKLPEGVKFEPADCAKFAEGQQFPPGLQGNMAATAAEGEGNRFIVMAVETSEPVPLSDPGDECKRVKFLGTGARGQVDVVESPQIDDARTVGTHRIIQTMR-----TGELYNYVASFDNYMVIVTANPLVLPDKPVAKVDTERARELLSAAVAAVRA

4tmdA

0.57

0.43

0.75

4.87

wPPAS

---------------------------------------------------------AYPDADIAKVAQLKSSFGPEFKVSEVAPTGIDPKLLSPQKLPEGVKFEPADCAKFAEGQQFPPGLQGNMAATAAEGEGNRFIVMAVETSEPVPLSDPGDECKRVKFLGTGARGQVDVVESPQIDDARTVGTHRIIQTMR-----TGELYNYVASFDNYMVIVTANPLVLPDKPVAKVDTERARELLSAAVAAVRA

4tmdA

0.57

0.43

0.75

6.02

dPPAS2

---------------------------------------------------------AYPDADIAKVAQLKSSFGPEFKVSEVAPTGIDPKLLSPQKLPEGVKFEPADCAKFAEGQQFPPGLQGNMAATAAEGEGNRFIVMAVETSEPVPLSDPGDECKRVKFLGTGARGQVDVVESPQIDDARTVGTHRIIQTMR-----TGELYNYVASFDNYMVIVTANPLVLPDKPVAKVDTERARELLSAAVAAVRA

4tmdA

0.57

0.43

0.75

4.03

PPAS

---------------------------------------------------------AYPDADIAKVAQLKSSFGPEFKVSEVAPTGIDPKLLSPQKLPEGVKFEPADCAKFAEGQQFPPGLQGNMAATAAEGEGNRFIVMAVETSEPVPLSDPGDECKRVKFLGTGARGQVDVVESPQIDDARTVGTHRIIQTMR-----TGELYNYVASFDNYMVIVTANPLVLPDKPVAKVDTERARELLSAAVAAVRA

4tmdA

0.57

0.43

0.75

4.33

Env-PPAS

---------------------------------------------------------AYPDADIAKVAQLKSSFGPEFKVSEVAPTGIDPKLLSPQKLPEGVKFEPADCAKFAEGQQFPPGLQGNMAATAAEGEGNRFIVMAVETSEPVPLSDPGDECKRVKFLGTGARGQVDVVESPQIDDARTVGTHRIIQTMR-----TGELYNYVASFDNYMVIVTANPLVLPDKPVAKVDTERARELLSAAVAAVRA

3sttA

0.10

0.92

0.69

MUSTER

VKKHFAF-HGAWCWYKIVALMRSSGINPKQALQIPNFSDYLSPLMEFMASLPANEKII--ALGGLAISKAMETFPEKISVAVFLSGLMPGPNIDATTVCTKAGSAVLGQLDNCVTYENGPTNPPTTLIAGPK-----FLATNVYHLSPIEDLALATALVRPLYLYLAEDIS----KEVVLSSKRYGSVKRVFIVATENDALKKEFLLMIEKNPPDEVKEI------EGSDHVTMKPQQLFTTLLSIANKYK-

4nx9A

0.09

0.08

0.93

0.69

dPPAS

----NGLNVATKNANDGISLAQTAEGALQQSTNILQRRDLSLQSAN-----GSNSDSERTALNQKELDRISNTLDGSFGVASFQVGSAANEIISVGIDESAESLNGTYFKADGGGAVTAATASGTVDIAIDITGGSAVNVKVDKGNAAKIAAAVNDANVGIGAFTDGAQISYVSKASADGTTSAVSGVAITDTGSTGAGT--------AAGTTTFTEANDTVAKIDISTAKGAQSAVLVIDEAIKQIDAQRA

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.27

Estimated TM-score = 0.56±0.15

Estimated RMSD = 8.6±4.5Å

Download Model 2

C-score=-2.43

Download Model 3

C-score=-2.90

Download Model 4

C-score=-2.74

Download Model 5

C-score=-2.06

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4tmdA	0.748	0.58	0.574	0.754
2	4esqA	0.505	3.94	0.114	0.659
3	2futA	0.470	5.18	0.071	0.702
4	3a0oA	0.465	4.95	0.101	0.675
5	1j0nA	0.456	5.27	0.056	0.682
6	5fjsA1	0.453	4.97	0.058	0.667
7	2cqtA	0.453	4.87	0.053	0.647
8	3dwkC2	0.452	5.61	0.030	0.702
9	5jmdA	0.452	5.07	0.070	0.655
10	1rw9A	0.450	5.26	0.066	0.691

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.17	8	4eneA	DMU	Rep, Mult	243,247
2	0.04	2	5hhjA	GLY	Rep, Mult	242,245
3	0.04	2	2xnvF	VU3	Rep, Mult	138,217,219
4	0.04	2	2c5wB	III	Rep, Mult	11,14,120,121,122,123,124,125,126,127,128,129
5	0.02	1	2jkgA	MG	Rep, Mult	178,181
6	0.02	1	2y6pC	MG	Rep, Mult	100,235
7	0.02	1	4fmsA	C8E	Rep, Mult	208,210,219
8	0.02	1	2cqtA	PO4	Rep, Mult	208,236,237
9	0.02	1	1izcA	MG	Rep, Mult	144,207
10	0.02	1	2cixA	MAN	Rep, Mult	185,186
11	0.02	1	5k1nB	RE	Rep, Mult	140,215
12	0.02	1	2hf8A	ZN	Rep, Mult	50,109
13	0.02	1	1agmA	MAN	Rep, Mult	103,106
14	0.02	1	1dq8D	COA	Rep, Mult	76,78

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	3imhA	0.403	5.29	0.039	0.619	5.1.3.3	NA
2	0.060	1z45A	0.437	5.28	0.051	0.659	5.1.3.2,5.1.3.3	208
3	0.060	1lf6A	0.389	5.16	0.055	0.579	3.2.1.3	77
4	0.060	1oqzB	0.392	5.96	0.059	0.647	3.5.1.93	NA
5	0.060	1l7kB	0.413	5.46	0.043	0.639	5.1.3.3	NA
6	0.060	3eqvA	0.428	5.74	0.047	0.679	3.4.16.4	187
7	0.060	2dqmA	0.419	5.83	0.038	0.691	3.4.11.2	NA
8	0.060	3fwlA	0.396	5.82	0.030	0.651	2.4.1.129	NA
9	0.060	3eifA	0.406	6.27	0.050	0.738	3.4.21.110	NA
10	0.060	1f1sA	0.435	5.27	0.051	0.647	4.2.2.1	NA
11	0.060	3mwxA	0.397	5.63	0.047	0.615	5.1.3.3	NA
12	0.060	2htaA	0.410	5.19	0.051	0.607	5.1.3.-	NA
13	0.060	1ffuB	0.395	6.00	0.034	0.675	1.2.99.2	NA
14	0.060	2wafA	0.406	6.05	0.043	0.706	3.4.-.-	NA
15	0.060	1rw9A	0.450	5.26	0.066	0.691	4.2.2.5	NA
16	0.060	2cqsA	0.451	5.02	0.058	0.655	2.4.1.20	NA
17	0.060	1i8qA	0.437	5.32	0.056	0.651	4.2.2.1	244
18	0.060	1snzA	0.411	5.49	0.080	0.631	5.1.3.3	NA
19	0.060	1sqiA	0.346	5.84	0.064	0.564	1.13.11.27	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.16	0.505	3.94	0.11	0.66	4esqA	GO:0000166 GO:0004672 GO:0004674 GO:0005524 GO:0005618 GO:0005886 GO:0005887 GO:0006468 GO:0016020 GO:0016021 GO:0016301 GO:0016310 GO:0016740 GO:0043085 GO:0043086 GO:0043388 GO:0045893 GO:0045926 GO:0046777 GO:0046890 GO:0052572
1	0.07	0.472	4.97	0.10	0.69	3aflA	GO:0016829
2	0.06	0.474	5.22	0.08	0.71	3e80A	GO:0008201 GO:0015021 GO:0016829 GO:0030211 GO:0042597 GO:0046872 GO:0047488
3	0.06	0.437	5.13	0.07	0.64	5jmfA	GO:0016829 GO:0042597
4	0.06	0.452	5.07	0.07	0.65	5jmdA	GO:0016829 GO:0042597
5	0.06	0.323	6.17	0.05	0.58	3w9aB	GO:0004553 GO:0005576 GO:0005975 GO:0030248 GO:0042802
6	0.06	0.329	5.61	0.10	0.52	4n0qB	GO:0006810 GO:0006865
7	0.06	0.321	6.47	0.04	0.60	1np7A	GO:0003677 GO:0003913 GO:0006281 GO:0006351 GO:0006355 GO:0018298
8	0.06	0.384	5.84	0.05	0.61	4fnvA	GO:0015021 GO:0016829 GO:0042597
9	0.06	0.301	6.17	0.05	0.50	2cl2A	GO:0004553 GO:0005975
10	0.06	0.273	6.51	0.07	0.50	1b0aA	GO:0000105 GO:0003824 GO:0004477 GO:0004488 GO:0005829 GO:0006164 GO:0006730 GO:0008152 GO:0008652 GO:0009086 GO:0009396 GO:0016491 GO:0016787 GO:0035999 GO:0055114
11	0.06	0.312	5.70	0.04	0.51	2ykkA	GO:0004553 GO:0005975 GO:0006080 GO:0016985 GO:0030246 GO:0030248 GO:0046872
12	0.06	0.262	5.53	0.06	0.42	2ws2A	GO:0016740
13	0.06	0.276	5.25	0.07	0.43	2hyeB	GO:0003723 GO:0016032 GO:0030430 GO:0030683 GO:0039502 GO:0039503 GO:0039554 GO:0039563 GO:0046872
14	0.06	0.254	5.64	0.04	0.41	3etpA	GO:0000166 GO:0000902 GO:0001525 GO:0001655 GO:0004672 GO:0004713 GO:0004714 GO:0004872 GO:0005003 GO:0005005 GO:0005102 GO:0005524 GO:0005886 GO:0005887 GO:0006468 GO:0007169 GO:0007275 GO:0007399 GO:0007411 GO:0007413 GO:0007612 GO:0008046 GO:0009887 GO:0016020 GO:0016021 GO:0016301 GO:0016310 GO:0016740 GO:0018108 GO:0021631 GO:0021952 GO:0022038 GO:0030424 GO:0030425 GO:0031290 GO:0042472 GO:0042802 GO:0042995 GO:0043025 GO:0045202 GO:0048013 GO:0048168 GO:0048170 GO:0048593 GO:0050770 GO:0050771 GO:0050878 GO:0051965 GO:0060021 GO:0060996 GO:0060997 GO:0071679
15	0.06	0.278	6.37	0.03	0.50	1h76A	GO:0005576 GO:0005615 GO:0005623 GO:0006810 GO:0006811 GO:0006826 GO:0006879 GO:0008199 GO:0015091 GO:0015682 GO:0046872 GO:0055072
16	0.06	0.235	6.27	0.06	0.41	1nzbA	GO:0003677 GO:0006310 GO:0015074
17	0.06	0.251	6.23	0.02	0.44	1pszA	GO:0005886 GO:0006810 GO:0007155 GO:0016020 GO:0030001 GO:0046872
18	0.06	0.263	5.58	0.06	0.42	3ivlA	GO:0003824 GO:0004222 GO:0006508 GO:0008233 GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0016773	GO:0032550	GO:0032559	GO:0035639	GO:0016301
GO-Score	0.32	0.32	0.32	0.32	0.32
Biological Processes	GO:0051101	GO:0051099	GO:0040008	GO:0048519	GO:0052200	GO:0019216	GO:1903508	GO:0006468	GO:0008610	GO:0006355	GO:0010628	GO:0052564	GO:0050790	GO:0044092
GO-Score	0.32	0.32	0.32	0.32	0.32	0.32	0.32	0.32	0.32	0.32	0.32	0.32	0.32	0.32
Cellular Component	GO:0030312	GO:0031226
GO-Score	0.32	0.32

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.