I-TASSER results

I-TASSER results for job id Rv0959

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (672 residues)
MAKSDGDDPLRPASPRLRSSRRHSLRYSAYTGGPDPLAPPVDLRDALEQIGQDVMAGASP
RRALSELLRRGTRNLTGADRLAAEVNRRRRELLRRNNLDGTLQEIKKLLDEAVLAERKEL
ARALDDDARFAELQLDALPASPAKAVQELAEYRWRSGQAREKYEQIKDLLGRELLDQRFA
GMKQALAGATDDDRRRVTEMLDDLNDLLDKHARGEDTQRDFDEFMTKHGEFFPENPRNVE
ELLDSLAKRAAAAQRFRNSLSQEQRDELDALAQQAFGSPALMRALDRLDAHLQAARPGED
WTGSQQFSGDNPFGMGEGTQALADIAELEQLAEQLSQSYPGASMDDVDLDALARQLGDQA
AVDARTLAELERALVNQGFLDRGSDGQWRLSPKAMRRLGETALRDVAQQLSGRHGERDHR
RAGAAGELTGATRPWQFGDTEPWHVARTLTNAVLRQAAAVHDRIRITVEDVEVAETETRT
QAAVALLVDTSFSMVMENRWLPMKRTALALHHLVCTRFRSDALQIIAFGRYARTVTAAEL
TGLAGVYEQGTNLHHALALAGRHLRRHAGAQPVVLVVTDGEPTAHLEDFDGDGTSVFFDY
PPHPRTIAHTVRGFDDMARLGAQVTIFRLGSDPGLARFIDQVARRVQGRVVVPDLDGLGA
AVVGDYLRFRRR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MAKSDGDDPLRPASPRLRSSRRHSLRYSAYTGGPDPLAPPVDLRDALEQIGQDVMAGASPRRALSELLRRGTRNLTGADRLAAEVNRRRRELLRRNNLDGTLQEIKKLLDEAVLAERKELARALDDDARFAELQLDALPASPAKAVQELAEYRWRSGQAREKYEQIKDLLGRELLDQRFAGMKQALAGATDDDRRRVTEMLDDLNDLLDKHARGEDTQRDFDEFMTKHGEFFPENPRNVEELLDSLAKRAAAAQRFRNSLSQEQRDELDALAQQAFGSPALMRALDRLDAHLQAARPGEDWTGSQQFSGDNPFGMGEGTQALADIAELEQLAEQLSQSYPGASMDDVDLDALARQLGDQAAVDARTLAELERALVNQGFLDRGSDGQWRLSPKAMRRLGETALRDVAQQLSGRHGERDHRRAGAAGELTGATRPWQFGDTEPWHVARTLTNAVLRQAAAVHDRIRITVEDVEVAETETRTQAAVALLVDTSFSMVMENRWLPMKRTALALHHLVCTRFRSDALQIIAFGRYARTVTAAELTGLAGVYEQGTNLHHALALAGRHLRRHAGAQPVVLVVTDGEPTAHLEDFDGDGTSVFFDYPPHPRTIAHTVRGFDDMARLGAQVTIFRLGSDPGLARFIDQVARRVQGRVVVPDLDGLGAAVVGDYLRFRRR
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHCCEEEEEHCCCCCCEEEEEEHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHCCCEEEHCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEHCCCCHHHHHHHHHHHHHCCEEEHCCHHHHHHHHHHHHHHHHCC
Conf.Score	988888888777776667788876656777898888888577999999999999868889999999999766885589999999999999999985677899999999999999999999875557999999999975575999999999866767999999999999999999999999999999959999999999999999999999998576799999999998655788866699999999999999999999878999999999999998579999999999999999966666666545456667767899999999999999999999986588754578899999979788887999999999999989865578886886889999999999999999865678887777667777667766677789888887899999999986678888789974648999865766656999997876666668589999999999999998688987999986896799889887765678889877999999999999978899868999978998777888888865445678998699999999999999879959999968885799999999999898899958777889999999998669
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MAKSDGDDPLRPASPRLRSSRRHSLRYSAYTGGPDPLAPPVDLRDALEQIGQDVMAGASPRRALSELLRRGTRNLTGADRLAAEVNRRRRELLRRNNLDGTLQEIKKLLDEAVLAERKELARALDDDARFAELQLDALPASPAKAVQELAEYRWRSGQAREKYEQIKDLLGRELLDQRFAGMKQALAGATDDDRRRVTEMLDDLNDLLDKHARGEDTQRDFDEFMTKHGEFFPENPRNVEELLDSLAKRAAAAQRFRNSLSQEQRDELDALAQQAFGSPALMRALDRLDAHLQAARPGEDWTGSQQFSGDNPFGMGEGTQALADIAELEQLAEQLSQSYPGASMDDVDLDALARQLGDQAAVDARTLAELERALVNQGFLDRGSDGQWRLSPKAMRRLGETALRDVAQQLSGRHGERDHRRAGAAGELTGATRPWQFGDTEPWHVARTLTNAVLRQAAAVHDRIRITVEDVEVAETETRTQAAVALLVDTSFSMVMENRWLPMKRTALALHHLVCTRFRSDALQIIAFGRYARTVTAAELTGLAGVYEQGTNLHHALALAGRHLRRHAGAQPVVLVVTDGEPTAHLEDFDGDGTSVFFDYPPHPRTIAHTVRGFDDMARLGAQVTIFRLGSDPGLARFIDQVARRVQGRVVVPDLDGLGAAVVGDYLRFRRR
Prediction	745554542142034424543441210121442120002244044004400520264541251045014304653620440154045215403644525420540352045004501441364445434324440551465145115405614342560254154035304552245315304531563456225304500540451055345455346304501551452237415304401540453444154135403550353044015411634503510440264046324635354445144544442542250253054045015204512554434414263036314562333353045105304732114434624140043004201430044005414634142524441434444343341411224302013002200223246463303033510312325533200000000000002243014102300200230046313501000000233024032530340343252100002003202520463653200000000010002124443334212132113240032004003402626020000000313202400320063050100002063012000200154358
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660

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Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHCCEEEEEHCCCCCCEEEEEEHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHCCCEEEHCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEHCCCCHHHHHHHHHHHHHCCEEEHCCHHHHHHHHHHHHHHHHCC
MAKSDGDDPLRPASPRLRSSRRHSLRYSAYTGGPDPLAPPVDLRDALEQIGQDVMAGASPRRALSELLRRGTRNLTGADRLAAEVNRRRRELLRRNNLDGTLQEIKKLLDEAVLAERKELARALDDDARFAELQLDALPASPAKAVQELAEYRWRSGQAREKYEQIKDLLGRELLDQRFAGMKQALAGATDDDRRRVTEMLDDLNDLLDKHARGEDTQRDFDEFMTKHGEFFPENPRNVEELLDSLAKRAAAAQRFRNSLSQEQRDELDALAQQAFGSPALMRALDRLDAHLQAARPGEDWTGSQQFSGDNPFGMGEGTQALADIAELEQLAEQLSQSYPGASMDDVDLDALARQLGDQAAVDARTLAELERALVNQGFLDRGSDGQWRLSPKAMRRLGETALRDVAQQLSGRHGERDHRRAGAAGELTGATRPWQFGDTEPWHVARTLTNAVLRQAAAVHDRIRITVEDVEVAETETRTQAAVALLVDTSFSMVMENRWLPMKRTALALHHLVCTRFRSDALQIIAFGRYARTVTAAELTGLAGVYEQGTNLHHALALAGRHLRRHAGAQPVVLVVTDGEPTAHLEDFDGDGTSVFFDYPPHPRTIAHTVRGFDDMARLGAQVTIFRLGSDPGLARFIDQVARRVQGRVVVPDLDGLGAAVVGDYLRFRRR

4uxvA

0.09

0.07

0.75

1.06

dPPAS

WKIEILNRSIVEEMSKIKHLKMTGQ--------------TEEFFEKWREEWDEIVTAHMPKVEELLYDAEENADKYRFKKANQVLVHIDDLLTAAESSIEKILREISDLVTSEEKSREEIEQ-VRERYSKSRKNLLAYSHLYGELYDSLEKDLDEIWYITARKVLLEQDRNLERLQSYIDDVPKLLADCKQTVPGQIAKLKDGYGEMKEKKLEHIQLDKELENLSNQLKRA--EHVLMTELDIDEASAILQLIDENIQSVYQQLEGEVEAGQSVLSKMPELIIAYDKLKEEKEHTKAETELVKESYRLTAGELG--KQQAFEKRLDEIGKLLSSVKDKLDAEHVAYSLLVEEVASIEKQIEEVKKEHAEYREALRKEELQARETLSNLKKTISETARLLKTSNIPGIPSHIQEMLENAHHHIQETVNQLNELPLNMEEAGAHLKQAEDIVNRASRESEELVEQVILIEKIIQFGNRFRSQNHILSEQLKEAERRFYAFDYDDSEIAAAAVEKAAPGAVEKD-------------------------------------------------------------------------------------------------------------------------------------------------------

5a8jA1

0.20

0.05

0.24

1.14

wdPPAS

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGIHYVVMIDNSPSMKKENKINLALSSASRLVQDII---PGNFISIYLFSNDIETLYEGE-IELKSIMGYTTNLHKAITKVLEKFKSSEIPV-KIILLSDGKPTDKRY----------------------SRDYESLQVPKNVQLITIGLGEDY--EAIMKILADKGSGVFYHIDPSQLPTTLVEQ------K

5j1iA

0.10

0.05

0.51

1.87

dPPAS2

----------------------------------------SRCQRCISELKDIRLQLEACETRTVHRLRLPL-DKEPARECAQRIAEQQKAQAEVEGLGKGVARLSAEAEKVLAL--SPAAPTLRSELELTLGKLEQVRSLSAIYLEKLKTISLVIRGTQGAEEVLRAHEEQLKEAQAVPATLPELEAT-KASLKKLRAQAEAQQPTFDALRDELRGAQEVGERLQQRHGERDVEVERWRERVAQLLERWQAVLAQTDVRQRELEQLGRQLRYYRESADPLGAWLQDARRRQEQIQAMDSQAVREQLRQEQAL-LEEIERHGEKVEECQRFAKQYINAIKDYELQLVTYKAQLEKVQSGSESVIQEYVDLRTHYSEL----------TTLTSQYIKFISET-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

2x31A

0.19

0.05

0.26

1.25

wPPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KEMSDRVLIFAVDASGSA---AVLSEAKGAVELLLGRA--YAARDHVSLITFRGTAAQVLKRQLQGLP--GGGGTPLASGMEMAMVTAKQSRGMTPTIALLTDGRGNIALDGT-----------ANRELAGEQATKVARAIRASGMPAVIIDTAMRP--NPALVDLARTMDAHYIA--LPRATAHKMADVLGAALE

1b3uA

0.13

0.11

0.86

1.09

MUSTER

-AAADGDDSLYP--AVLIDELRN----------ED----VQLRLNSIKKLSTIALAL-GVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEPSDLEAHFVPLVKRLAGWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLAS------DEQDSVRLLAVEACVNIAQLLP------QEDLEALVMPTLRQAAESWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPA-----VELAEDAKWRVRLAIIEYMPLLAGQLGVEFF----------DEKLNSLCMAWLVDHV--------YAIREAATSNLKKLVEKFGKEW----------AHATIIPKVLAMDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTV------------------LRMAGDPVANVRFNVAKSLQKIGPI--------LDNSTLQSEVKPILEKLTQ------DQDVDVKYFAQEALTVLSL

5a8jA1

0.19

0.04

0.24

2.14

Env-PPAS

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGIHYVVMIDNSPSMKKENKINLALSSASRLVQDII---PGNFISIYLFSNDIETLYEGESGKQIELMGYTTNLHKAITKVLEKFKSSE-IPVKIILLSDGKPTDKRY----------------------SRDYESLQVPKNVQLITIGLGEDY-NEAIMKILADKGSGVFYHIDPSQLPTTLVEQK------

5j1iA

0.10

0.05

0.51

1.05

dPPAS

----------------------------------------SRCQRCISELKDIRLQLEACETRTVHRLRLPLDKE-PARECAQRIAEQQKAQAEVEGLGKGVARLSAEAEKVLAL--SPAAPTLRSELELTLGKLEQVRSLSAIYLEKLKTISLVIRGTQGAEEVLRAHEEQLKEAQAVPATLPELEAT-KASLKKLRAQAEAQQPTFDALRDELRGAQEVGERLQQRHGERDVEVERWRERVAQLLERWQAVLAQTDVRQRELEQLGRQLRYYRESADPLGAWLQDARRRQEQIQAMDSQAVREQLRQEQAL-LEEIERHGEKVEECQRFAKQYINAIKDYELQLVTYKAQLEKVQSGSESVIQEYVDLRTHYSEL----------TTLTSQYIKFISET-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

4ui9O

0.10

0.09

0.90

1.08

wMUSTER

MSRTGEGAVSLVERRRLNQQLLPLLQ------GPDI--TVSKLLKLIEESCPQLAN---SVQIRIKLMAEG--ELKDMEQFFDDLSDSFSGTEPEVHLRHMILAYSKLSFSQVFKLYTALQQYFQSDENKKQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFN-PDFAEAHYLSYLNNLRVQDFSSTHSLLHYFDRLAESKSNGEEGYGAALNLAALHCRFGHY-QQAELALQEAIRIAQESHVCLQHCLSWLRSDSYVLLEHSVKKAVHFSLVQQRAFAANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEQNNTESFAVALCHLAELHAEQFAAASEVLKHL---KERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITALNS-----------------IEGVYRKAVVLQAQNQSEAHKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKEY--RLQ--------YLASETVLNLAFAQLILGIPEQALSLLHMAIEPILADGAILDK-AMFLVAKCQVASAASYDQPK--------KAEALEAAIENLNEAKNY--------VDCKERIRDVVYFQARLYHGK------TQERNRCAMLFRQLHQE

5j1iA

0.12

0.06

0.51

1.14

wdPPAS

-------------------------------------SRCQRCISELKDIRLQLEA---CETRTVHRLR--PLDKEPARECAQRIAEQQKAQAEVEGLGKGVARLSAEAEKVLALAAPTLRSEL-ELTLGKLEQVRSLSAIYLEKLKTISLVIRGTQGAEEVLRAHEEQLKEA---QAVPATLPELEATKASLKKLRAQAEAQQPTFDALRDELRGAQEVGERLQQRHGERDVE-VERWRERVAQLLERWQAVLAQTDVRQRELEQLGRQLRYYRESADPLGAWLQDARRRQEQIQAMDSQAVREQLRQEQAL-LEEIERHGEKVEECQRFAKQYINAIKDYELQLVTYKAQLEKVQSGSESVIQEYVDLRTHYSEL----------TTLTSQYIKFISET-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

5a8jA1

0.21

0.05

0.23

1.20

wPPAS

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGIHYVVMIDNSPSMKKENKINLALSSASRLVQDII---PGNFISIYLFSNDIETLYEIELKSIKM--GYTTNLHKAITKVLEKFKSSEIPV-KIILLSDGKPT--------DKRY--------------SRDYESLQVPKNVQLITIGLGEDY--EAIMKILADKGSGVFYH-DPSQLPTTLVEQ------K

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.99

Estimated TM-score = 0.48±0.15

Estimated RMSD = 12.8±4.2Å

Download Model 2

C-score=-2.74

Download Model 3

C-score=-3.17

Download Model 4

C-score=-4.60

Download Model 5

C-score=-3.10

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1b3uA	0.821	2.06	0.114	0.862
2	1vsy5	0.642	6.22	0.068	0.878
3	5dlqB	0.584	5.76	0.082	0.771
4	3ea5B	0.582	5.88	0.060	0.771
5	1vsy4	0.575	5.79	0.057	0.749
6	3icqT	0.573	5.89	0.071	0.777
7	3gjxA	0.564	6.39	0.056	0.775
8	4knhA	0.559	6.18	0.073	0.766
9	4ifqA	0.554	6.56	0.061	0.780
10	4c0oA	0.552	6.17	0.068	0.749

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.11	6	3q3gI	CA	Rep, Mult	489,491,493,551,579
2	0.08	4	1shtX	MG	Rep, Mult	491,493,551
3	0.04	2	1o6pA	III	Rep, Mult	481,484,485,543,551,552
4	0.04	2	3q0gA	COA	Rep, Mult	224,227
5	0.02	1	4jyxC	UNL	Rep, Mult	222,226
6	0.02	1	4rfuB	CA	Rep, Mult	470,471
7	0.02	1	4d2eC	78M	Rep, Mult	510,514
8	0.02	1	3n8nB	N88	Rep, Mult	402,405
9	0.02	1	2rh1A	CLR	Rep, Mult	487,510
10	0.02	1	5klaA	NUC	Rep, Mult	124,129,157,160,161,196,198,199,202,244,286,290,322,323,325,326,365,366,368,369,372,402,404,405,408,455
11	0.02	1	2q5dB	III	Rep, Mult	448,451,508
12	0.02	1	2z66A	UUU	Rep, Mult	525,527,541
13	0.02	1	1r2rB	DMS	Rep, Mult	195,198,244,248
14	0.02	1	1f59A	III	Rep, Mult	269,272,273,276,277,304,308,312
15	0.02	1	1u6g0	III	Rep, Mult	440,446,450,491,503
16	0.02	1	1jkjA	COA	Rep, Mult	218,221

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1d8dA	0.346	5.05	0.088	0.438	2.5.1.58	NA
2	0.060	2np0A	0.332	8.63	0.032	0.567	3.4.24.69	612
3	0.060	1fo4A	0.329	8.31	0.051	0.540	1.17.1.4	NA
4	0.060	1kc7A	0.335	8.17	0.047	0.548	2.7.9.1	NA
5	0.060	3btaA	0.338	8.34	0.037	0.559	3.4.24.69	NA
6	0.060	3dpyA	0.345	5.16	0.074	0.442	2.5.1.58,2.5.1.59	487
7	0.060	3c5wA	0.293	3.29	0.113	0.329	3.1.3.16	488,547
8	0.060	3b9jI	0.117	5.23	0.050	0.149	1.17.1.4,1.17.3.2	NA
9	0.060	2x0sA	0.335	8.43	0.035	0.554	2.7.9.1	NA
10	0.060	1slyA	0.324	7.14	0.068	0.485	4.2.2.-	507
11	0.060	1kblA	0.337	8.17	0.043	0.551	2.7.9.1	NA
12	0.060	1l5jA	0.344	7.79	0.031	0.539	4.2.1.3	NA
13	0.060	1z1wA	0.338	7.24	0.039	0.498	3.4.11.-	NA
14	0.060	3h09B	0.369	7.79	0.048	0.576	3.4.21.72	NA
15	0.060	2ckjA	0.328	8.25	0.047	0.534	1.17.1.4,1.17.3.2	NA
16	0.060	2pffD	0.319	8.76	0.020	0.552	2.3.1.86	486,525
17	0.060	1z0hB	0.163	7.72	0.026	0.252	3.4.24.69	552
18	0.060	3ecqB	0.376	8.60	0.038	0.635	3.2.1.97	NA
19	0.060	1ug9A	0.290	7.77	0.037	0.449	3.2.1.70	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.18	0.821	2.06	0.11	0.86	1b3uA	GO:0000086 GO:0000159 GO:0000184 GO:0000188 GO:0000775 GO:0003823 GO:0004722 GO:0005634 GO:0005694 GO:0005737 GO:0005739 GO:0005829 GO:0006275 GO:0006355 GO:0006461 GO:0006470 GO:0006672 GO:0006915 GO:0007059 GO:0007084 GO:0007143 GO:0008380 GO:0008601 GO:0010033 GO:0015630 GO:0016020 GO:0019932 GO:0030111 GO:0030155 GO:0030308 GO:0034047 GO:0040008 GO:0042518 GO:0045595 GO:0046982 GO:0051232 GO:0051306 GO:0051754 GO:0070062 GO:0070262 GO:1903538 GO:2001241
1	0.07	0.642	6.19	0.07	0.88	4v7oA6	GO:0000502 GO:0005634 GO:0005737 GO:0005839 GO:0006281 GO:0006974 GO:0010499 GO:0010952 GO:0016504 GO:0034515 GO:0043248 GO:0043486 GO:0061136 GO:0070577 GO:0070628 GO:1990236 GO:1990237
2	0.07	0.587	5.24	0.07	0.75	4a0cA	GO:0000151 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0010265 GO:0016020 GO:0016567 GO:0017025 GO:0030154 GO:0031461 GO:0043086 GO:0045893 GO:0045899 GO:0070062
3	0.07	0.540	5.99	0.06	0.72	4xriA	GO:0005622 GO:0006886 GO:0008536
4	0.07	0.547	5.39	0.06	0.70	3nd2A	GO:0000059 GO:0000060 GO:0000176 GO:0005087 GO:0005634 GO:0005643 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006612 GO:0006656 GO:0006810 GO:0006886 GO:0008139 GO:0008536 GO:0008565 GO:0015031 GO:0031965 GO:0034399 GO:0043547 GO:0051028 GO:0051292 GO:0060188
5	0.07	0.535	5.73	0.08	0.70	1qgkA	GO:0000059 GO:0000060 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006309 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0007079 GO:0007080 GO:0008139 GO:0008270 GO:0008536 GO:0008565 GO:0015031 GO:0016020 GO:0016032 GO:0019054 GO:0019899 GO:0019904 GO:0030953 GO:0031291 GO:0031965 GO:0034399 GO:0040001 GO:0043234 GO:0044822 GO:0045184 GO:0051879 GO:0070062 GO:0071782 GO:0075733 GO:0090307
6	0.07	0.529	6.29	0.06	0.72	2q5dB	GO:0000059 GO:0000060 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006309 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0007079 GO:0007080 GO:0008139 GO:0008270 GO:0008536 GO:0008565 GO:0015031 GO:0016020 GO:0016032 GO:0019054 GO:0019899 GO:0019904 GO:0030953 GO:0031291 GO:0031965 GO:0034399 GO:0040001 GO:0043234 GO:0044822 GO:0045184 GO:0051879 GO:0070062 GO:0071782 GO:0075733 GO:0090307
7	0.06	0.387	6.19	0.07	0.53	4uadA	GO:0005634 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006606 GO:0006607 GO:0006810 GO:0008139 GO:0008565 GO:0015031 GO:0016020 GO:0045944 GO:0060135
8	0.06	0.374	6.13	0.07	0.52	4rxhB	GO:0005634 GO:0005737 GO:0006606 GO:0006810 GO:0008565 GO:0015031
9	0.06	0.369	6.30	0.07	0.52	4b8jA	GO:0005634 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006606 GO:0006607 GO:0006810 GO:0008139 GO:0008565 GO:0015031 GO:0016032 GO:0048471
10	0.06	0.361	6.02	0.07	0.49	1wa5B	GO:0000176 GO:0005634 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006606 GO:0006607 GO:0006612 GO:0006810 GO:0008139 GO:0008565 GO:0015031 GO:0031144 GO:0048471
11	0.06	0.329	3.94	0.07	0.38	1oyzA	GO:0006974 GO:0046677
12	0.06	0.294	3.35	0.12	0.33	4lacA	GO:0000086 GO:0000159 GO:0000184 GO:0000188 GO:0000775 GO:0003823 GO:0004722 GO:0005634 GO:0005694 GO:0005737 GO:0005739 GO:0005829 GO:0006275 GO:0006355 GO:0006461 GO:0006470 GO:0006672 GO:0006915 GO:0007059 GO:0007084 GO:0007143 GO:0008380 GO:0008601 GO:0010033 GO:0015630 GO:0016020 GO:0019932 GO:0030111 GO:0030155 GO:0030308 GO:0034047 GO:0040008 GO:0042518 GO:0045595 GO:0046982 GO:0051232 GO:0051306 GO:0051754 GO:0070062 GO:0070262 GO:1903538 GO:2001241
13	0.06	0.293	3.43	0.09	0.33	1lrvA
14	0.06	0.273	4.72	0.06	0.33	3ebbA	GO:0006644 GO:0006954 GO:0007165 GO:0016005 GO:0043085 GO:0070062
15	0.06	0.277	4.26	0.08	0.33	4g3aB	GO:0000022 GO:0000070 GO:0000775 GO:0000776 GO:0000922 GO:0005525 GO:0005634 GO:0005737 GO:0005813 GO:0005815 GO:0005819 GO:0005827 GO:0005856 GO:0005874 GO:0005875 GO:0005876 GO:0007049 GO:0007051 GO:0007052 GO:0007067 GO:0007275 GO:0007282 GO:0007411 GO:0008017 GO:0016325 GO:0019827 GO:0030154 GO:0030426 GO:0030723 GO:0031116 GO:0032154 GO:0035099 GO:0035371 GO:0040001 GO:0042995 GO:0045169 GO:0045172 GO:0046580 GO:0046602 GO:0048477 GO:0051225 GO:0051301 GO:0051315 GO:0090307
16	0.06	0.255	4.73	0.10	0.32	4qmhA	GO:0000022 GO:0000132 GO:0000922 GO:0005813 GO:0005875 GO:0007017 GO:0007029 GO:0007067 GO:0007143 GO:0007344 GO:0007411 GO:0008017 GO:0015631 GO:0016325 GO:0031122 GO:0035371 GO:0045196 GO:0045450 GO:0048477 GO:0090063
17	0.06	0.246	3.72	0.10	0.28	3ltjA
18	0.06	0.207	7.87	0.03	0.33	2ymwB	GO:0005576 GO:0016491 GO:0031640 GO:0033736 GO:0042742 GO:0048038 GO:0055114 GO:1900191

Consensus prediction of GO terms

Molecular Function	GO:0004721	GO:0019888	GO:0046983
GO-Score	0.36	0.36	0.36
Biological Processes	GO:0001558	GO:0051304	GO:0007155	GO:0042221	GO:0051231	GO:0016055	GO:0043666	GO:1903506	GO:0042516	GO:0000070	GO:0006665	GO:0045926	GO:0051445	GO:0051052	GO:0031468	GO:0000956	GO:0043407	GO:0071822	GO:0070601	GO:1903537	GO:0065003	GO:2001239	GO:2001238	GO:0010468	GO:0000212	GO:2000112	GO:0070271	GO:0043603	GO:0051177	GO:0042532	GO:0044772	GO:0006351	GO:0044839	GO:0006260	GO:1900193	GO:0006396	GO:0010564
GO-Score	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36
Cellular Component	GO:0031988	GO:1903561	GO:0098687	GO:0008287	GO:0044444	GO:0005856	GO:0005634
GO-Score	0.47	0.47	0.36	0.36	0.36	0.36	0.33

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.