I-TASSER results

I-TASSER results for job id Rv0877

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (262 residues)
MTGPTEESAVATVADWPEGLAAVLRGAADQARAAVVEFSGPEAVGDYLGVSYEDGNAATH
RFIAHLPGYQGWQWAVVVASYSGADHATISEVVLVPGPTALLAPDWVPWEQRVRPGDLSP
GDLLAPAKDDPRLVPGYTASGDAQVDETAAEIGLGRRWVMSAWGRAQSAQRWHDGDYGPG
SAMARSTKRVCRDCGFFLPLAGSLGAMFGVCGNELSADGHVVDRQYGCGAHSDTTAPAGG
STPIYEPYDDGVLDIIEKPAES

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MTGPTEESAVATVADWPEGLAAVLRGAADQARAAVVEFSGPEAVGDYLGVSYEDGNAATHRFIAHLPGYQGWQWAVVVASYSGADHATISEVVLVPGPTALLAPDWVPWEQRVRPGDLSPGDLLAPAKDDPRLVPGYTASGDAQVDETAAEIGLGRRWVMSAWGRAQSAQRWHDGDYGPGSAMARSTKRVCRDCGFFLPLAGSLGAMFGVCGNELSADGHVVDRQYGCGAHSDTTAPAGGSTPIYEPYDDGVLDIIEKPAES
Prediction	CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHCCCEEEEEEHCCCCCCCCEEEEEEEEHCCCCCCEEEEEEEHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEHCCCCHHHHCCCCCCCCCCCCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHCCCCCC
Conf.Score	9988876654445567657899999999999999999768888865777899679578998764799999858999999778998656665665789987679998998886887889988788999999887887567887578899999889987547999999999999978999999888657778888567886577567655765456689986897545888777788899999988888888754444478779
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MTGPTEESAVATVADWPEGLAAVLRGAADQARAAVVEFSGPEAVGDYLGVSYEDGNAATHRFIAHLPGYQGWQWAVVVASYSGADHATISEVVLVPGPTALLAPDWVPWEQRVRPGDLSPGDLLAPAKDDPRLVPGYTASGDAQVDETAAEIGLGRRWVMSAWGRAQSAQRWHDGDYGPGSAMARSTKRVCRDCGFFLPLAGSLGAMFGVCGNELSADGHVVDRQYGCGAHSDTTAPAGGSTPIYEPYDDGVLDIIEKPAES
Prediction	7634367423443463264024104501520340036126673004112133535300101010533415302000000104744401001000222660120172121641143440333231236663520352245564541452144141224300155016301610473743162630541543031000102032304310000002124403001242101111525356665432342124211422543488
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHCCCEEEEEEHCCCCCCCCEEEEEEEEHCCCCCCEEEEEEEHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEHCCCCHHHHCCCCCCCCCCCCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHCCCCCC
MTGPTEESAVATVADWPEGLAAVLRGAADQARAAVVEFSGPEAVGDYLGVSYEDGNAATHRFIAHLPGYQGWQWAVVVASYSGADHATISEVVLVPGPTALLAPDWVPWEQRVRPGDLSPGDLLAPAKDDPRLVPGYTASGDAQVDETAAEIGLGRRWVMSAWGRAQSAQRWHDGDYGPGSAMARSTKRVCRDCGFFLPLAGSLGAMFGVCGNELSADGHVVDRQYGCGAHSDTTAPAGGSTPIYEPYDDGVLDIIEKPAES

1yt8A

0.15

0.14

0.95

0.64

MUSTER

GQQLEHGQTRRFGAISQDTRKAAAQRARAVADRAGVERLDLAGL---AQWQDEHDR-TTYLLDVRTPGHPGSRSTPQLVQETDHVASVRGARLVLVDDDGVRANSASWLAQGWQVAVLDGLSDFSERGAWSAPLPRQPRADTIDPTTLADWLGEPGTRVL----TA--SANYAKR-PGLRSQLKQALERLGTAERYVLTCGSSLLARFAVAEVQALSGKPVFLLDGGTSA-AGLPTEDGESLLASPRIDRRPYEGTDNPREA

5gaoE

0.16

0.15

0.94

0.98

dPPAS

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA---AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA--------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA------AAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

4lg3A

0.17

0.09

0.52

0.63

wdPPAS

KQGNYIARRDSSTVSKRQLAATVIAAARYYAQEKLDSQPGPAFGKTVLATATYAPD-------KGVSGSDDWTWNTLQAT---PRGLTAQELKI-----------QCLWGER-----------------GKFQVDGSTDE-RRLKAAIAKKLKIPAEKVLNPVFPEPFPQEWTR----------------------------------------------------------------------------------------

2p6pA

0.12

0.11

0.93

0.58

wMUSTER

RPEAIPSDPVAQARFTGRWFARMAASSLPRMLDFSRAWGGTMSYVAPLLALHLGVPHARQTWDAVDA--DGIHPGADAELRPGLERLPAPDLFIDICPPSLRPANAAP-ARMMRHATSRQCPLMYTRDTRQRRVAKESYDRNDFLRGLAKDLVWDVELIVA--PDTVAEALRAEVPWTPLDVVAPTCDLLVHHAG-----GVSTLTGLGV----KGSVARRVA-DYGAA----IALLPGEDSTCQELQAKDTYEISGMPLPA

4q0pA

0.23

0.18

0.79

0.65

wPPAS

-------SARTSITR--REYDEWVREAAALGKALRYPIT-EKMVNDSAGIVFGADQ-----YDAFKNGM--W------SGEP--EAMIIFESLNEPAVDGL--PT-APYAE---SGDVHPGKFCP---------PHHHGRKTESYEVVLGEMEVSGVELLNFSGMPVGS--WPEGVALPKGRESSYEKLT-----SYVRLAGDLH-AF--CPPD--SDVPLVVREVSTYSHEPTEAAAGNHAPIPSMHDN---DFVSDAANT

2mj6A

0.18

0.06

0.31

0.79

dPPAS2

--------GSETRTISSTAQERVDLEAVRLASIVDSRLIGTGSVDDFLREQIRDARYAVIRIPGQPVGTKG---DVLQGRAEEGETVLVEEPR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------

5gaoE

0.15

0.14

0.95

0.66

PPAS

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA---AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA---------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTK

3h41A

0.16

0.15

0.94

0.61

Env-PPAS

WTAPDSLRPIDVPSATPVDLWKWTKSTLDEKLWLTNANKLETQAGQEVTVVDKKGLVHGQPTPRNEEGYPGWPEKQLTYNQEFADK-TNEPFVLVTKPTAILYIN--P-SEKHKSLEVSYNTRLPLLSEDTISYRVLLPNGQKRKNDGTFYRSQNDIPTPAADDLINTGKIW--AGTSGFSGFTHTI---YKSHGITIPRDSGPQSRNGVDKEHLQK-GDLIFFAHDQGKHGNIHSPRAERSVEIIPLNTPARRYLP-----

2fcuA

0.09

0.94

0.60

MUSTER

LRLLDRSAAAYQDLDAISGGTNIANYDRHLDDDFLASHICRPEICDRVESIL-GPNVLCWRTEFKYPGDEGTDWHQADTFANASGKPQIIWPENEEFGGTITV--WTA--TDANI-ANGCLQFIPGTQNSMNYDETKRMTYEPDANNSVVKDGVRRGFF-----GYDYRQLQIDENWKPDEASAVPMQMKAGQFIIFWSTL-----MHASYPHSGESQEMGFASRY-VPSFVHVYPDSDHIEEYGGRISEKYGAVQVIGDET

5c2vC

0.09

0.08

0.90

0.76

dPPAS

STIQTGATWEPLGREEPLTVPEVHFELVRYGQFQFNDAASLQGSYSCASCHYERGQTTGLIWDLGDEGWGSWKNTKYIRGGRYLPPFR---HEGFTGHPDEIVGATSSLDRVCG-----------RDPGFVFRSENFSPMRLEALICYIRALEFTGSPFRNA-----------DGSLTEAQKRGQKPKVGCLECDPRALFSDAQTHDVGTGRVGVNGFRSTPGKVFNISALEAGEDPYGVESNTPIIGLDLVKEFDTPTLRD

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-4.73

Estimated TM-score = 0.23±0.06

Estimated RMSD = 17.9±2.5Å

Download Model 2

C-score=-5.00

Download Model 3

C-score=-5.00

Download Model 4

C-score=-5.00

Download Model 5

C-score=-4.61

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1yt8A	0.724	3.91	0.114	0.889
2	4xc6A	0.453	6.09	0.063	0.767
3	4iibA	0.448	5.52	0.056	0.710
4	4d0jA	0.448	5.59	0.060	0.710
5	5f0eA	0.447	5.61	0.051	0.706
6	2x2hA1	0.439	5.72	0.039	0.718
7	3tonA	0.438	5.55	0.048	0.695
8	3l4uA	0.435	5.66	0.043	0.702
9	1jqkA	0.434	5.72	0.048	0.706
10	3s1sA	0.431	5.11	0.057	0.653

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.07	5	4m8lC	ARF	Rep, Mult	181,184,185
2	0.06	4	3i04B	SF4	Rep, Mult	196,199,211
3	0.03	2	1vk6A	ZN	Rep, Mult	191,194,211,228
4	0.03	2	1focA	HEM	Rep, Mult	191,194
5	0.03	2	1jqkA	SF4	Rep, Mult	160,161,163,164,180,210
6	0.03	2	3oojC	GLU	Rep, Mult	65,66,68,124,125,129
7	0.03	2	1shkA	MG	Rep, Mult	214,217
8	0.01	1	2a68O	MG	Rep, Mult	91,96
9	0.01	1	1su6A	SF4	Rep, Mult	166,168,170,183
10	0.01	1	2ypaB	NUC	Rep, Mult	29,32
11	0.01	1	1o5qA	MG	Rep, Mult	159,224
12	0.01	1	1mjgA	SF4	Rep, Mult	79,80,83,84,85,114
13	0.01	1	2z8yC	SF4	Rep, Mult	83,88,91
14	0.01	1	1oaoA	SF4	Rep, Mult	66,78,85,256
15	0.01	1	5ioeB	ZN	Rep, Mult	25,29
16	0.01	1	1dxeA	MG	Rep, Mult	91,122
17	0.01	1	3tdvA	MG	Rep, Mult	231,249
18	0.01	1	3fyuC	XYP	Rep, Mult	232,233
19	0.01	1	3uilB	DAO	Rep, Mult	34,35
20	0.01	1	4toqA	MG	Rep, Mult	187,216

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	3dy5A	0.391	6.39	0.048	0.695	1.13.11.40,4.2.1.92	NA
2	0.060	1pj6A	0.417	6.02	0.068	0.702	1.5.3.10	NA
3	0.060	2nlkA	0.390	6.32	0.028	0.683	3.1.3.48	NA
4	0.060	2et6A	0.401	6.06	0.042	0.676	1.1.1.-	NA
5	0.060	1vlbA	0.369	6.36	0.037	0.660	1.2.99.7	164
6	0.060	1larA	0.404	6.30	0.068	0.702	3.1.3.48	NA
7	0.060	2q28A	0.396	5.26	0.065	0.588	4.1.1.8	71
8	0.060	1jqkF	0.435	5.69	0.052	0.706	1.2.99.2	203
9	0.060	2ow6A	0.395	6.37	0.039	0.695	3.2.1.114	46
10	0.060	1gz3A	0.409	5.39	0.056	0.649	1.1.1.38	NA
11	0.060	3cskA	0.394	6.22	0.050	0.683	3.4.14.4	NA
12	0.060	3fg3A	0.330	6.50	0.028	0.599	4.2.1.92,1.13.11.40	NA
13	0.060	3l4uA	0.435	5.66	0.043	0.702	3.2.1.20,3.2.1.3	184,188,197
14	0.060	2x0sA	0.388	5.73	0.041	0.622	2.7.9.1	NA
15	0.060	1o0sA	0.394	5.73	0.062	0.645	1.1.1.38	NA
16	0.060	2q5lA	0.390	5.45	0.078	0.588	4.1.1.74,4.1.1.43	NA
17	0.060	2j5wA	0.396	6.04	0.034	0.676	1.16.3.1	NA
18	0.060	2zuwC	0.413	5.80	0.058	0.676	2.4.1.211	182
19	0.060	2fh7A	0.309	6.09	0.040	0.534	3.1.3.48	176

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.13	0.728	3.93	0.11	0.89	1yt8A	GO:0004792 GO:0016740
1	0.06	0.380	5.82	0.05	0.61	3ippB	GO:0004792 GO:0016740 GO:0016783 GO:0042597
2	0.06	0.327	4.72	0.06	0.45	1h4kX	GO:0004792 GO:0005737 GO:0016740
3	0.06	0.328	5.15	0.06	0.49	1uarA	GO:0004792 GO:0016740
4	0.06	0.327	4.56	0.04	0.46	3hzuA	GO:0004792 GO:0005618 GO:0005829 GO:0005886 GO:0016740
5	0.06	0.337	5.74	0.05	0.53	4jgtA	GO:0001822 GO:0001889 GO:0004792 GO:0005737 GO:0005739 GO:0005829 GO:0009440 GO:0009636 GO:0016740 GO:0016784 GO:0019346 GO:0021510 GO:0030054 GO:0042802 GO:0043005 GO:0045202 GO:0070062 GO:0070814
6	0.06	0.323	5.08	0.08	0.48	3p3aA	GO:0004792 GO:0016740
7	0.06	0.301	4.18	0.02	0.39	2eg4A	GO:0004792 GO:0016740 GO:0046872
8	0.06	0.301	6.04	0.05	0.51	1fg3A	GO:0000105 GO:0003824 GO:0004400 GO:0005829 GO:0008152 GO:0008483 GO:0008652 GO:0009058 GO:0016740 GO:0030170 GO:0042802 GO:0080130
9	0.06	0.295	3.06	0.08	0.35	1gn0A	GO:0004792 GO:0005737 GO:0006071 GO:0016740
10	0.06	0.392	5.56	0.05	0.61	4qq8C	GO:0000287 GO:0003824 GO:0016829 GO:0030976 GO:0046872
11	0.06	0.284	3.12	0.05	0.34	3d1pA	GO:0004792 GO:0005739 GO:0016740
12	0.06	0.340	5.22	0.03	0.51	3tp9A	GO:0046872
13	0.06	0.322	5.49	0.03	0.50	1urhA	GO:0004792 GO:0005737 GO:0005829 GO:0016740 GO:0016784 GO:0046677
14	0.06	0.279	3.20	0.09	0.34	2moiA	GO:0004792 GO:0005886 GO:0005887 GO:0016020 GO:0016021
15	0.06	0.198	6.81	0.03	0.37	3zpyA	GO:0016829 GO:0045135
16	0.06	0.279	6.89	0.04	0.53	1k3vA	GO:0005198 GO:0016032 GO:0019012 GO:0019028 GO:0019062 GO:0039615 GO:0039665 GO:0042025 GO:0046718 GO:0046872 GO:0075509 GO:0075512 GO:0075521 GO:0075606 GO:0075732
17	0.06	0.256	4.02	0.05	0.35	1t3kA	GO:0004721 GO:0004725 GO:0005634 GO:0005739 GO:0006468 GO:0006470 GO:0007049 GO:0007067 GO:0009507 GO:0016491 GO:0016787 GO:0035335 GO:0046685 GO:0046872 GO:0051301 GO:0055114
18	0.06	0.234	2.07	0.07	0.26	2jtqA	GO:0004792 GO:0016740 GO:0030288 GO:0042597

Consensus prediction of GO terms

Molecular Function	GO:0004792
GO-Score	0.33
Biological Processes
GO-Score
Cellular Component	GO:0005886	GO:0042597	GO:0005829	GO:0005618
GO-Score	0.06	0.06	0.06	0.06

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.