I-TASSER results

I-TASSER results for job id Rv0784

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (228 residues)
MSVSGIGESTLADVDAFCAEMDARSVPVSLLVAPRMRDDYRLDRDPRTVDWLTGRRAAGD
ALVLHGYDEAATKRRRGEFAMLRAHEANLRLMAADRVLEHLGLRTRLFAAPGWLVSPGVR
TALPANGFRLLADLHGITDLVRLTTVRARVLGIGEGFLAEPWWCRMVVMSAERIARRGGV
VRIAVAARHLRKSGPLQAMLDAVDLAMLQGCTPMVYRWRADAAVLDAA

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MSVSGIGESTLADVDAFCAEMDARSVPVSLLVAPRMRDDYRLDRDPRTVDWLTGRRAAGDALVLHGYDEAATKRRRGEFAMLRAHEANLRLMAADRVLEHLGLRTRLFAAPGWLVSPGVRTALPANGFRLLADLHGITDLVRLTTVRARVLGIGEGFLAEPWWCRMVVMSAERIARRGGVVRIAVAARHLRKSGPLQAMLDAVDLAMLQGCTPMVYRWRADAAVLDAA
Prediction	CCHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCCHHHHHHHHHHCCEEEHCCCCEEHCCCCEEEHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEHCHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEHCCCCHHHHCCC
Conf.Score	955578985789999999999998998689986678998765559799999999999898899857778887777456665999999999999999999989987577588644898999999997998996677567668995887665555667655557889999999999865895899985877688668999999999999989865555556654544579
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MSVSGIGESTLADVDAFCAEMDARSVPVSLLVAPRMRDDYRLDRDPRTVDWLTGRRAAGDALVLHGYDEAATKRRRGEFAMLRAHEANLRLMAADRVLEHLGLRTRLFAAPGWLVSPGVRTALPANGFRLLADLHGITDLVRLTTVRARVLGIGEGFLAEPWWCRMVVMSAERIARRGGVVRIAVAARHLRKSGPLQAMLDAVDLAMLQGCTPMVYRWRADAAVLDAA
Prediction	430000234226404400520473703000000022366241662550152045127332100000110343654421025154510341054025005726140400000012125501510472603101223102304644324030112244232332113102311122247430000000053054652152024003302734141333534444424748
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCCHHHHHHHHHHCCEEEHCCCCEEHCCCCEEEHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEHCHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEHCCCCHHHHCCC
MSVSGIGESTLADVDAFCAEMDARSVPVSLLVAPRMRDDYRLDRDPRTVDWLTGRRAAGDALVLHGYDEAATKRRRGEFAMLRAHEANLRLMAADRVLEHLGLRTRLFAAPGWLVSPGVRTALPANGFRLLADLHGITDLVRLTTVRARVLGIGEGFLAEPWWCRMVVMSAERIARRGGVVRIAVAARHLRKSGPLQAMLDAVDLAMLQGCTPMVYRWRADAAVLDAA

3rxzA

0.15

0.13

0.88

1.19

MUSTER

TSHQA---GPLVGVPRLLGILDEFNVPGTFFVPGYTAHRH--------PEPIRSIARAGHEIAHHGYLHESLV-------GADEDTERKILTRGIEALEEV-VHPVGYRAPWE--NWHTPKLLAEFGFLYDSTLDSELAVGDGSLVELPVFSGTGLIETPAKAIELWRAELNAR-DIGGAWVLTNHPFLSGRPGRAAALREFIAEVCADD------VWVAGSQIAERA

3rxzA

0.16

0.14

0.90

1.46

dPPAS

RGTSHQAYGPLVGVPRLLGILDEFNVPGTFFVPGYTA--------HRHPEPIRSIARAGHEIAHHGYLHESLV-------GADEDTERKILTRGIEALEEVAVHPVGYRAPWENW--HTPKLLAEFGFLYDSTLDSELAVGDGSLVELPVSWAGTGLIETPAKAIELWRAELNARDIGGAWVLTNHPFLSGRPGRAAALREFIAEVCADD------VWVAGSQIAEHV

2cc0A

0.13

0.10

0.80

2.34

wdPPAS

LTFDD-GPS--GSTQSLLNALRQNGLRATMFNQGQYA--------AQNPSLVRAQVDAGMWVANHSYTHPH-------MTQLGQAQMDSEISRTQQAIAGAGGTPKLFRPPYGETNATLRSVEAKYGLTEVIWDVDSQDWNNAS-------------------TDAIVQAVSRL-GNGQ--VILMHD---WPANTLAAIPRIAQTLAGKGLCSGMISPQTGRAVAP--

3rxzA

0.15

0.13

0.88

1.60

wMUSTER

TSHQA---GPLVGVPRLLGILDEFNVPGTFFVPGYTAHRH--------PEPIRSIARAGHEIAHHGYLHESLVG-------ADEDTERKILTRGIEALEEV-VHPVGYRAPWE--NWHTPKLLAEFGFLYDSTLDSELAVGDGSLVELPVFSGTGLIETPAKAIELWRAELNAR-DIGGAWVLTNHPFLSGRPGRAAALREFIAEVCADD------VWVAGSQIAERA

2cc0A

0.13

0.11

0.80

2.55

wPPAS

VGL-DDGPS--GSTQSLLNALRQNGLRATMFNQGQYA--------AQNPSLVRAQVDAGMWVANHSYTHPH-------MTQLGQAQMDSEISRTQQAIAGAGGTPKLFRPPYGETNATLRSVEAKYGLTEVIWDVDSQDWNNAS-------------------TDAIVQAVSRL-GNGQ--VILMHD---WPANTLAAIPRIAQTLAGKGLCSGMISPQTGRAV---A

3rxzA

0.15

0.13

0.86

3.94

dPPAS2

--------GPLVGVPRLLGILDEFNVPGTFFVPGYTAHRH--------PEPIRSIARAGHEIAHHGYLHESLV-------GADEDTERKILTRGIEALEEVAVHPVGYRAPWE--NWHTPKLLAEFGFLYDSTLDSYELAVGDGSLVELPVSWGTGLIETPAKAIELWRAELNARDIGGAWVLTNHPFLSGRPGRAAALREFIAEVCADD------VWVAGSQIAEHV

3rxzA

0.14

0.12

0.89

1.77

PPAS

FADRGTS-GPLVGVPRLLGILDEFNVPGTFFVPGYTAHRH--------PEPIRSIARAGHEIAHHGYLHESLVG-------ADEDTERKILTRGIEALEEVAVHPVGYRAPWE--NWHTPKLLAEFGFLYDSTLDSYELAVGDQYCFVPDFSGTGLIETPAKAIELWRAELNAR-DIGGAWVLTNHPFLSGRPGRAAALREFIAEVCADD------VWVAGSQIAEHV

4m1bA

0.11

0.09

0.81

2.56

Env-PPAS

IYKGDTSKKQDKKAIPILDTLKERDIKATFFLSAAWA--------ERHPDVVERIIKDGHEIGSMGYNYTS-------YTSLETNEIRRDLLRAQDVFTKLVKQIKLLRPPSGDFNKATLKIAESLGYTVVHSNNSNWKNPG---------------------VNKIVSTVSNNLKGG--DIVLLHASD-SALQTNKALPLLLQKLKSDGYEQISV-----SQLISNT

3s6oA

0.16

0.14

0.89

1.10

MUSTER

QFLSEIVGASRAGVWRILREFDKRGLPLTVFGVGMAIERH--------PELARAFVELGHEIACHGWRWIHYQ-------DMTPEREAEHMRLGMEAIERV-VRPLGWYTGRD--SPNTHRLVAEYGFLYDSDHYGDVEVSGGASVPQLIVPYTLGFNTADHFFHYLRDAFDVLYEEGDMMSIGMHCRLLGRPGRFRALQRFLDHIERHDR-----VWVARRVEI-AR

3qbuA

0.12

0.11

0.91

1.34

dPPAS

LGSYGGEDSGEVGIPRLLKLFKKYHLPATWFVPGHSI--------ETFPEQMKMIVDAGHEVGAHGYSHENPIA-------MSTKQEEDVLLKSVELIKDLTKAPTGYVAPWWEFSNITNELLLKHGFKYDHSLMHNDFTPYYVRVGDKKSPNSFGFVSPRDIGQMWIDQFDWVYREMDVFSMTIHPDVSARPQVLLMHEKIIEHINKHEG-----VRWVTFNEIADD

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-0.36

Estimated TM-score = 0.67±0.13

Estimated RMSD = 6.4±3.9Å

Download Model 2

C-score=-2.13

Download Model 3

C-score=-2.43

Download Model 4

C-score=-3.52

Download Model 5

C-score=-2.61

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3rxzA	0.806	2.26	0.145	0.908
2	3qbuA	0.801	2.46	0.119	0.921
3	3s6oA	0.792	2.50	0.129	0.917
4	3n8tA	0.775	3.08	0.112	0.943
5	2b5dX	0.770	3.30	0.095	0.965
6	1z7aF	0.768	2.53	0.142	0.895
7	1ufaA	0.762	3.08	0.127	0.934
8	3lm3A	0.750	3.18	0.128	0.925
9	4cmrA	0.741	3.40	0.079	0.939
10	1k1yA1	0.739	3.28	0.103	0.934

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.26	4	2cc0A	ACT	Rep, Mult	5,65,69,109,110,111,112,134,186
2	0.13	2	2nt0D	GOL	Rep, Mult	18,22,25,26,56,59,60
3	0.06	1	3rxzA	ZN	Rep, Mult	65,69
4	0.06	1	2v3eA	NND	Rep, Mult	89,91,122

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.203	2iw0A	0.725	2.84	0.108	0.842	3.5.1.41	67,189
2	0.158	2w3zA	0.706	2.89	0.138	0.829	3.5.1.41	112
3	0.110	1c92A	0.616	4.13	0.101	0.851	3.2.1.96	107
4	0.067	1v5xA	0.541	3.47	0.067	0.689	5.3.1.24	NA
5	0.067	2cz5A	0.544	3.86	0.068	0.737	4.1.1.23	NA
6	0.066	2czfA	0.527	3.92	0.057	0.728	4.1.1.23	31,58,65
7	0.066	2v81A	0.511	4.29	0.060	0.728	4.1.2.21	NA
8	0.066	1c3fA	0.620	4.04	0.101	0.851	3.2.1.96	NA
9	0.066	1nsjA	0.534	3.58	0.043	0.693	5.3.1.24	133
10	0.066	1xi3A	0.527	3.66	0.054	0.697	2.5.1.3	NA
11	0.066	1w0mA	0.544	4.04	0.068	0.746	5.3.1.1	NA
12	0.066	1xbzB	0.534	4.26	0.040	0.754	4.1.1.85	123
13	0.066	1u83A	0.550	3.81	0.069	0.737	4.4.1.19	NA
14	0.066	1xi3B	0.527	3.67	0.054	0.697	2.5.1.3	66
15	0.066	1vqtA	0.492	3.95	0.058	0.675	4.1.1.23	NA
16	0.060	1ll6A	0.658	4.03	0.099	0.903	3.2.1.14	19
17	0.060	2epoB	0.653	4.07	0.076	0.903	3.2.1.52	NA
18	0.060	2fhcA	0.634	4.74	0.078	0.943	3.2.1.41	33,89,133
19	0.060	1fa2A	0.657	4.17	0.067	0.908	3.2.1.2	NA
20	0.060	2gjxA	0.636	4.24	0.058	0.903	3.2.1.52	NA
21	0.060	2wyhA	0.672	3.99	0.113	0.925	3.2.1.24	38
22	0.060	1v03A	0.619	4.64	0.052	0.912	3.2.1.21	NA
23	0.060	1w9pA	0.660	4.05	0.108	0.903	3.2.1.14	NA
24	0.060	3gh4A	0.651	4.17	0.058	0.917	3.2.1.52	NA
25	0.060	1gonA	0.635	4.11	0.058	0.886	3.2.1.21	98
26	0.060	2ow6A	0.710	3.83	0.074	0.943	3.2.1.114	NA
27	0.060	1d2kA	0.655	4.05	0.094	0.903	3.2.1.14	NA
28	0.060	1b1yA	0.664	4.03	0.053	0.903	3.2.1.2	13
29	0.060	1xc6A	0.655	4.34	0.045	0.930	3.2.1.23	92
30	0.060	1wdpA	0.655	4.07	0.063	0.895	3.2.1.2	45
31	0.060	2e9bA	0.639	4.66	0.100	0.939	3.2.1.41	82,105,127,129
32	0.060	2epoA	0.662	4.05	0.062	0.908	3.2.1.52	NA
33	0.060	2fhbA	0.633	4.73	0.069	0.943	3.2.1.41	NA
34	0.060	1hp4A	0.645	4.18	0.031	0.912	3.2.1.52	60

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.31	0.791	2.49	0.14	0.92	1z7aA	GO:0003824 GO:0005975 GO:0016810
1	0.29	0.802	2.45	0.12	0.92	4ly4A	GO:0000272 GO:0003824 GO:0005975 GO:0016787 GO:0016810 GO:0045493 GO:0046555 GO:0046872 GO:0071555
2	0.25	0.713	2.38	0.11	0.82	2vyoA	GO:0003824 GO:0005975 GO:0016810
3	0.25	0.725	2.84	0.11	0.84	2iw0A	GO:0003824 GO:0004099 GO:0005975 GO:0016787 GO:0016810 GO:0046872
4	0.24	0.475	5.04	0.05	0.74	5jp6A	GO:0003824 GO:0005975 GO:0016787 GO:0016810
5	0.21	0.690	2.66	0.11	0.80	2cc0A	GO:0003824 GO:0004553 GO:0005975 GO:0016810 GO:0030246 GO:0030247 GO:0046872
6	0.20	0.714	2.22	0.12	0.80	4m1bA	GO:0003824 GO:0005975 GO:0016810
7	0.17	0.823	2.25	0.14	0.93	3rxzC	GO:0003824 GO:0005975 GO:0016810 GO:0046872
8	0.16	0.712	2.25	0.09	0.79	4l1gA	GO:0003824 GO:0005975 GO:0016787 GO:0016810
9	0.16	0.788	2.54	0.15	0.92	3cl6A	GO:0003824 GO:0005975 GO:0016810
10	0.15	0.792	2.50	0.13	0.92	3s6oC	GO:0003824 GO:0005975 GO:0016810
11	0.15	0.737	2.99	0.12	0.88	3wx7A	GO:0003824 GO:0004553 GO:0005576 GO:0005975 GO:0016810 GO:0030246 GO:0046872
12	0.12	0.706	2.89	0.14	0.83	2w3zA	GO:0003824 GO:0005975 GO:0016810 GO:0046872
13	0.11	0.704	2.58	0.10	0.81	2c71A	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0016810 GO:0030246 GO:0045493
14	0.11	0.704	2.54	0.10	0.82	2y8uB	GO:0003824 GO:0004099 GO:0005975 GO:0016810
15	0.10	0.702	2.35	0.10	0.79	1ny1A	GO:0003824 GO:0005975 GO:0016787 GO:0016810 GO:0030435 GO:0046872 GO:0071555
16	0.10	0.703	2.40	0.12	0.80	2c1gA	GO:0003824 GO:0005886 GO:0005975 GO:0009405 GO:0016020 GO:0016021 GO:0016787 GO:0016810 GO:0046872
17	0.08	0.658	2.20	0.11	0.74	2j13A	GO:0003824 GO:0005975 GO:0016810 GO:0019213
18	0.07	0.617	4.29	0.07	0.88	3hqpA	GO:0000166 GO:0000287 GO:0003824 GO:0004743 GO:0005524 GO:0006096 GO:0016301 GO:0016310 GO:0016740 GO:0030955 GO:0046872
19	0.07	0.736	3.02	0.11	0.88	4nyuA	GO:0003824 GO:0004099 GO:0004553 GO:0005576 GO:0005975 GO:0016787 GO:0016810 GO:0030246
20	0.06	0.354	5.27	0.07	0.57	1n1zA	GO:0000287 GO:0008152 GO:0009507 GO:0009536 GO:0010333 GO:0016829 GO:0016853 GO:0046211 GO:0046872 GO:0047926
21	0.06	0.391	5.31	0.05	0.63	2gjtA	GO:0000902 GO:0002548 GO:0003093 GO:0003105 GO:0004721 GO:0004725 GO:0005001 GO:0005886 GO:0005887 GO:0006470 GO:0007411 GO:0010812 GO:0010977 GO:0016020 GO:0016021 GO:0016311 GO:0016324 GO:0016328 GO:0016787 GO:0016791 GO:0017147 GO:0030027 GO:0030032 GO:0030424 GO:0030426 GO:0032835 GO:0035335 GO:0036060 GO:0042803 GO:0043005 GO:0043197 GO:0070062 GO:0072112 GO:0090090 GO:0090260

Consensus prediction of GO terms

Molecular Function	GO:0052689	GO:0016811	GO:0019213	GO:0046872
GO-Score	0.58	0.49	0.49	0.46
Biological Processes	GO:0000272	GO:0045229	GO:0045491
GO-Score	0.58	0.58	0.58
Cellular Component
GO-Score

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.