I-TASSER results

I-TASSER results for job id Rv0647c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (488 residues)
MRAEIGPDFRPHYTFGDAYPASERAHVNWELSAPVWHTAQMGSTTHREVAKLDRVPLPVE
AARVAATGWQVTRTAVRFIGRLPRKGPWQQKVIKELPQTFADLGPTYVKFGQIIASSPGA
FGESLSREFRGLLDRVPPAKTDEVHKLFVEELGDEPARLFASFEEEPFASASIAQVHYAT
LRSGEEVVVKIQRPGIRRRVAADLQILKRFAQTVELAKLGRRLSAQDVVADFADNLAEEL
DFRLEAQSMEAWVSHLHASPLGKNIRVPQVHWDFTTERVLTMERVHGIRIDNAAAIRKAG
FDGVELVKALLFSVFEGGLRHGLFHGDLHAGNLYVDEAGRIVFFDFGIMGRIDPRTRWLL
RELVYALLVKKDHAAAGKIVVLMGAVGTMKPETQAAKDLERFATPLTMQSLGDMSYADIG
RQLSALADAYDVKLPRELVLIGKQFLYVERYMKLLAPRWQMMSDPQLTGYFANFMVEVSR
EHQSDIEV

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MRAEIGPDFRPHYTFGDAYPASERAHVNWELSAPVWHTAQMGSTTHREVAKLDRVPLPVEAARVAATGWQVTRTAVRFIGRLPRKGPWQQKVIKELPQTFADLGPTYVKFGQIIASSPGAFGESLSREFRGLLDRVPPAKTDEVHKLFVEELGDEPARLFASFEEEPFASASIAQVHYATLRSGEEVVVKIQRPGIRRRVAADLQILKRFAQTVELAKLGRRLSAQDVVADFADNLAEELDFRLEAQSMEAWVSHLHASPLGKNIRVPQVHWDFTTERVLTMERVHGIRIDNAAAIRKAGFDGVELVKALLFSVFEGGLRHGLFHGDLHAGNLYVDEAGRIVFFDFGIMGRIDPRTRWLLRELVYALLVKKDHAAAGKIVVLMGAVGTMKPETQAAKDLERFATPLTMQSLGDMSYADIGRQLSALADAYDVKLPRELVLIGKQFLYVERYMKLLAPRWQMMSDPQLTGYFANFMVEVSREHQSDIEV
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHEEEEEHCCCCCEEEEEEHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHCEEEHHHCCCCEEEEEEHCCEHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHCCCCCEEEEHCCCCEHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHCHHHHHCCC
Conf.Score	98877888776666777788776667654445546677765666545565555555678899999999999999999877655555666568999999999989845688899986888898999999999976699999999999999998999999987758875355544445678868997899998589989999999999999999999856656999999999999999999979999999999999996798878688776754448897568776688778889999998999999999999999999999798678999976898699969999568956679999999999999999979899999999998998999999999999999999986899778899999999999999979878858989999999999999986999864444799999999985765778876689
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MRAEIGPDFRPHYTFGDAYPASERAHVNWELSAPVWHTAQMGSTTHREVAKLDRVPLPVEAARVAATGWQVTRTAVRFIGRLPRKGPWQQKVIKELPQTFADLGPTYVKFGQIIASSPGAFGESLSREFRGLLDRVPPAKTDEVHKLFVEELGDEPARLFASFEEEPFASASIAQVHYATLRSGEEVVVKIQRPGIRRRVAADLQILKRFAQTVELAKLGRRLSAQDVVADFADNLAEELDFRLEAQSMEAWVSHLHASPLGKNIRVPQVHWDFTTERVLTMERVHGIRIDNAAAIRKAGFDGVELVKALLFSVFEGGLRHGLFHGDLHAGNLYVDEAGRIVFFDFGIMGRIDPRTRWLLRELVYALLVKKDHAAAGKIVVLMGAVGTMKPETQAAKDLERFATPLTMQSLGDMSYADIGRQLSALADAYDVKLPRELVLIGKQFLYVERYMKLLAPRWQMMSDPQLTGYFANFMVEVSREHQSDIEV
Prediction	75463445144332244513345334231421230220141233232220302212100002000000110021003002324545512551042014002713100001001000032001640051045012502414273024002630544044005402630000004002010203534400010121403500410030021002003104404704043004101610251033432050043016105725435302002001111263330021034140532530473623044003100200010002100100201000000055120000000000203660031002001000134005200400230001356142630250024004403734054130340042004004617040100000201010000000120014010013120350036203630453346175
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHEEEEEHCCCCCEEEEEEHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHCEEEHHHCCCCEEEEEEHCCEHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHCCCCCEEEEHCCCCEHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHCHHHHHCCC
MRAEIGPDFRPHYTFGDAYPASERAHVNWELSAPVWHTAQMGSTTHREVAKLDRVPLPVEAARVAATGWQVTRTAVRFIGRLPRKGPWQQKVIKELPQTFADLGPTYVKFGQIIASSPGAFGESLSREFRGLLDRVPPAKTDEVHKLFVEELGDEPARLFASFEEEPFASASIAQVHYATLRSGEEVVVKIQRPGIRRRVAADLQILKRFAQTVELAKLGRRLSAQDVVADFADNLAEELDFRLEAQSMEAWVSHLHASPLGKNIRVPQVHWDFTTERVLTMERVHGIRIDNAAAIRKAGFDGVELVKALLFSVFEGGLRHGLFHGDLHAGNLYVDEAGRIVFFDFGIMGRIDPRTRWLLRELVYALLVKKDHAAAGKIVVLMGAVGTMKPETQAAKDLERFATPLTMQSLGDMSYADIGRQLSALADAYDVKLPRELVLIGKQFLYVERYMKLLAPRWQMMSDPQLTGYFANFMVEVSREHQSDIEV

4pedA

0.20

0.15

0.75

2.67

MUSTER

------------------------------------------------------------------------------------------ANAERIVRTLCKVRGAALKLGQLSIQDDAFINPHLAKIFERVRQ-SADFP--LKQKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLAR--KGGREVAKIQYPGVAQSINSDVNNLAVLNSNL-----PEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHP---FFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEI--RNEICYNILVLCLRELFEF-HFQTDPNWSNFFYDPQHKVALLDFGATREYDRSFTDLYIQIIRAAADR-DRETVRAKSIEKFLTGYEVKEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPRHRLVPPPEETYSLHRKGGSFLICSKLKARFPC--KAFEEAYSNYCKRQ-----------

4pedA

0.20

0.15

0.75

4.87

dPPAS

------------------------------------------------------------------------------------------ANAERIVRTLCKVRGAALKLGQLSIQDDAFINPHLAKIFERVRQSAD---FPLKQKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARK--GGREVAKIQYPGVAQSINSDVNNLAVLNSNL-----PEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHP---FFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEI--RNEICYNILVLCLRELFEF-HFQTDPNWSNFFYDPQHKVALLDFGATREYDRSFTDLYIQIIRAAADR-DRETVRAKSIEKFLTGYEVKEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPRHRLVPPPEETYSLHRKGGSFLICSKLKARFPC--KAFEEAYSNYCKRQ-----------

4pedA

0.20

0.15

0.75

7.07

wdPPAS

------------------------------------------------------------------------------------------ANAERIVRTLCKVRGAALKLGQLSIQDDAFINPHLAKIFERVRQSADF---PLKQKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARK--GGREVAKIQYPGVAQSINSDVNNLAVLNSNL-----PEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHP---FFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEI--RNEICYNILVLCLRELFE--HFQTDPNWSNFFYDPQHKVALLDFGATREYDRSFTDLYIQIIRAAADR-DRETVRAKSIEKFLTGYEVKEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLILRHRLVPPPEETYSLHRKGGSFLICSKLKARFPC--KAFEEAYSNYCKRQ-----------

4pedA

0.20

0.15

0.75

3.30

wMUSTER

------------------------------------------------------------------------------------------ANAERIVRTLCKVRGAALKLGQLSIQDDAFINPHLAKIFERVRQS-ADFP--LKQKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLAR--KGGREVAKIQYPGVAQSINSDVNNLAVLNSNL-----PEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHP---FFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEI--RNEICYNILVLCLRELFEF-HFQTDPNWSNFFYDPQHKVALLDFGATREYDRSFTDLYIQIIRAAADR-DRETVRAKSIEKFLTGYEVKEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPRHRLVPPPEETYSLHRKGGSFLICSKLKARFPC--KAFEEAYSNYCKRQ-----------

4pedA

0.20

0.15

0.75

4.08

wPPAS

------------------------------------------------------------------------------------------ANAERIVRTLCKVRGAALKLGQLSIQDDAFINPHLAKIFERVRQSAD-FP--LKQKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARK--GGREVAKIQYPGVAQSINSDVNNLAVLNSNL-----PEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHP---FFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEI--RNEICYNILVLCLRELFE--HFQTDPNWSNFFYDPQHKVALLDFGATREYDRSFTDLYIQIIRAAADR-DRETVRAKSIEKFLTGYEVKEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPRHRLVPPPEETYSLHRKGGSFLICSKLKARFPCKA--FEEAYSNYCKRQ-----------

4pedA

0.20

0.15

0.75

12.09

dPPAS2

------------------------------------------------------------------------------------------ANAERIVRTLCKVRGAALKLGQLSIQDDAFINPHLAKIFERVRQSAD---FPLKQKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARK--GGREVAKIQYPGVAQSINSDVNNLAVLNSNL-----PEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHP---FFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEI--RNEICYNILVLCLRELFEF-HFQTDPNWSNFFYDPQHKVALLDFGATREYDRSFTDLYIQIIRAAADR-DRETVRAKSIEKFLTGYEVKEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPRHRLVPPPEETYSLHRKGGSFLICSKLKARFPC--KAFEEAYSNYCKRQ-----------

4pedA

0.20

0.15

0.75

3.93

PPAS

------------------------------------------------------------------------------------------ANAERIVRTLCKVRGAALKLGQLSIQDDAFINPHLAKIFERVRQS-ADFP--LKQKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARK--GGREVAKIQYPGVAQSINSDVNNLAVLNSNL-----PEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHP---FFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEI--RNEICYNILVLCLRELFEF-HFQTDPNWSNFFYDPQHKVALLDFGATREYDRSFTDLYIQIIRAAADR-DRETVRAKSIEKFLTGYEVKEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPRHRLVPPPEETYSLHRKGGSFLICSKLKARFPC--KAFEEAYSNYCKRQ-----------

4pedA

0.20

0.15

0.75

8.14

Env-PPAS

------------------------------------------------------------------------------------------ANAERIVRTLCKVRGAALKLGQLSIQDDAFINPHLAKIFERVRQS-ADFP--LKQKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARK--GGREVAKIQYPGVAQSINSDVNNLAVLNSNL-----PEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHP---FFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEI--RNEICYNILVLCLRELFEF-HFQTDPNWSNFFYDPQHKVALLDFGATREYDRSFTDLYIQIIRAAADR-DRETVRAKSIEKFLTGYEVKEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPRHRLVPPPEETYSLHRKGGSFLICSKLKARFPCKA--FEEAYSNYCKRQ-----------

2ynsA

0.13

0.10

0.77

0.79

MUSTER

LPAMIG--------------YSDDNNLQLEATTQFRKLLSIERSPP--IEEVIQSGVVPRFVQFLTRFPQLQFEAAWALTNIASGT-------SENTKVVIDHG-AVPIFVKLLGS-SS---DDVREQAVWALGNVAGDSPKCRDLVLANGA---LLPLLAQLNEH-----------------------------------TKLSMLRNATWTLSNFCRGKPQPSFEQTRPALPALARLIDEEVLTDACWALSYLSDGT-------------------------------DKIQAVIEAGV-CPRLVELLLPSVLIPALRT--------VGNIVTGDDQTQCIIDHQALPCLKKSIKKEACWTISNITAGN--KDQIQAVINAGIIGQTAEFDIKKEAAWAISNATSGGSHDQI-KYLVSEGCIKPLCDL-LICP-DIRIVTVCLEGLENILKVGEGDVNVFSQMIDEAEGLEKIENLQSNEIYEKKI

4widA

0.05

0.03

0.68

0.65

dPPAS

LEKMLADEEKLTEFNLGD---PLFESANDDPIKTLEEIIQEGDDVVGAHQLVVTQIKLRVQRNRRLADEIIREQLTDIRKVFSDKFEKLEQGIQNSYLLLDKLKTPFQDMRCLFEVANEQFNDTPVPPQYK-------EFMVCLKQIVQYAVNSSSLEKFVMLI--------------------------------TKKDDIKDRVTYTCMKYLLMAMQGTGGPKAINNEEHAKLFFQYDDLTDANHDGLELIKKLDKEQ-----------KEV---------------AFHVNNFTHLVTTLGMALYKEGHQKNDEAMLG--------------------------------MHTPITMLSDQVRVLILYLIDEIVHAIHTNSNQSNDELIDGLKPKVRIVINEFHATLMMGIDKMKFLNELREIVNDKI---------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.90

Estimated TM-score = 0.49±0.15

Estimated RMSD = 11.8±4.5Å

Download Model 2

C-score=-2.21

Download Model 3

C-score=-2.62

Download Model 4

C-score=-2.77

Download Model 5

C-score=-3.11

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4pedA	0.734	1.38	0.198	0.754
2	2x0oA	0.419	5.32	0.084	0.564
3	3to3A	0.414	5.29	0.058	0.551
4	2w02B	0.403	5.30	0.065	0.527
5	3c3lA	0.391	7.17	0.048	0.625
6	5jm7A	0.386	5.00	0.070	0.498
7	5a22A	0.379	7.28	0.056	0.611
8	1vw1A	0.368	6.66	0.052	0.566
9	4xhjA	0.367	6.82	0.033	0.568
10	1g5aA	0.367	7.28	0.049	0.592

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.20	12	5i35A	ANP	Rep, Mult	167,169,171,172,176,188,190,282,283,285,332,334,344,345
2	0.12	7	4j99D	MG	Rep, Mult	190,245,345,347
3	0.12	7	3g15A	MG	Rep, Mult	332,345
4	0.03	2	1q97B	ADN	Rep, Mult	170,176,188,268,283,284,285
5	0.02	1	1l8tA	MG	Rep, Mult	172,345
6	0.02	1	3c46A	2HP	Rep, Mult	250,269,270,271

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2b9iA	0.353	5.24	0.098	0.453	2.7.11.24,2.7.1.37	327,332
2	0.060	2fhbA	0.315	7.37	0.030	0.516	3.2.1.41	NA
3	0.060	3k2lA	0.352	5.67	0.076	0.469	2.7.12.1	327,332
4	0.060	2jiiB	0.356	4.71	0.095	0.451	2.7.11.1	NA
5	0.060	2vx3A	0.351	5.13	0.098	0.455	2.7.12.1	241,278
6	0.060	3kx2B	0.364	7.46	0.048	0.607	3.6.4.13	131
7	0.060	2b4kA	0.301	7.56	0.054	0.506	3.1.1.43	329
8	0.060	2fhcA	0.351	7.48	0.051	0.580	3.2.1.41	334
9	0.060	1mpxA	0.345	7.28	0.039	0.566	3.2.1.43	NA
10	0.060	2excX	0.330	5.37	0.075	0.432	2.7.11.24,2.7.1.37	327,332
11	0.060	1nx9A	0.355	7.03	0.066	0.568	3.1.1.43	194
12	0.060	3e7oA	0.337	5.15	0.105	0.441	2.7.11.24	327,332
13	0.060	1kblA	0.299	7.69	0.055	0.504	2.7.9.1	NA
14	0.060	2eabA	0.350	7.36	0.037	0.578	3.2.1.63	NA
15	0.060	3c46B	0.353	7.36	0.044	0.576	2.7.7.6	234
16	0.060	2vd5A	0.349	4.73	0.084	0.436	2.7.11.1	327,332
17	0.060	1s0kA	0.358	7.48	0.040	0.600	3.2.1.18	332
18	0.060	2wu6A	0.345	5.32	0.098	0.451	2.7.12.1	327,332
19	0.060	3ebgA	0.352	7.38	0.046	0.586	3.4.11.-	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.25	0.751	1.47	0.20	0.77	4pedA	GO:0000166 GO:0005524 GO:0005739 GO:0006744 GO:0016020 GO:0016021 GO:0016301 GO:0016310 GO:0016740 GO:0043531
1	0.06	0.424	5.26	0.05	0.57	3to3B	GO:0000166 GO:0005524 GO:0005623 GO:0015343 GO:0019290 GO:0044718 GO:0046872
2	0.06	0.420	5.42	0.08	0.57	2x0qA	GO:0000166 GO:0005524 GO:0005623 GO:0015343 GO:0019290 GO:0044718 GO:0046872
3	0.06	0.302	7.63	0.04	0.51	2va8B
4	0.06	0.287	7.82	0.03	0.49	4ml8A	GO:0000166 GO:0003824 GO:0009690 GO:0016491 GO:0016614 GO:0019139 GO:0050660 GO:0055114
5	0.06	0.289	7.60	0.03	0.49	4mlaB	GO:0000166 GO:0003824 GO:0009690 GO:0016491 GO:0016614 GO:0019139 GO:0050660 GO:0055114
6	0.06	0.406	5.29	0.06	0.53	2x3jA	GO:0000166 GO:0005524 GO:0005623 GO:0015343 GO:0019290 GO:0044718 GO:0046872
7	0.06	0.254	7.53	0.05	0.42	5jpiA	GO:0004013 GO:0006730 GO:0016787
8	0.06	0.232	7.27	0.04	0.38	3h9mA	GO:0009058 GO:0016829
9	0.06	0.272	7.10	0.04	0.44	2j37W	GO:0000166 GO:0003723 GO:0003924 GO:0005525 GO:0005634 GO:0005730 GO:0005737 GO:0005786 GO:0005829 GO:0006614 GO:0006616 GO:0006617 GO:0008144 GO:0008312 GO:0019003 GO:0030529 GO:0030942 GO:0042493 GO:0043021 GO:0044822 GO:0045047 GO:0048500
10	0.06	0.234	7.50	0.04	0.39	2zwyA	GO:0004560 GO:0005975 GO:0006004
11	0.06	0.239	6.75	0.05	0.36	3ab4A	GO:0000166 GO:0004072 GO:0005524 GO:0008152 GO:0008652 GO:0009085 GO:0009088 GO:0009089 GO:0016301 GO:0016310 GO:0016597 GO:0016740 GO:0019877
12	0.06	0.223	7.69	0.04	0.38	3d6kC	GO:0003824
13	0.06	0.221	6.25	0.04	0.33	5fsrA	GO:0004175 GO:0004180 GO:0005886 GO:0005887 GO:0006508 GO:0008233 GO:0008360 GO:0008658 GO:0009002 GO:0009252 GO:0016020 GO:0016787 GO:0071555
14	0.06	0.221	6.57	0.07	0.34	2dt8A	GO:0008289
15	0.06	0.209	7.06	0.04	0.33	1o8cA	GO:0005737 GO:0008270 GO:0016491 GO:0043957 GO:0055114
16	0.06	0.208	6.72	0.01	0.32	3j7y7	GO:0000002 GO:0000166 GO:0003723 GO:0004827 GO:0005739 GO:0005743 GO:0005761 GO:0005840 GO:0006418 GO:0006433 GO:0016876 GO:0030529 GO:0044822 GO:0070125 GO:0070126
17	0.06	0.203	6.67	0.07	0.32	1ycdB	GO:0005634 GO:0005737 GO:0016787 GO:0052689
18	0.06	0.180	5.66	0.06	0.25	4gjhC	GO:0004842 GO:0004871 GO:0005737 GO:0005813 GO:0005829 GO:0006915 GO:0007165 GO:0008270 GO:0008284 GO:0009898 GO:0016567 GO:0031625 GO:0031996 GO:0035631 GO:0042981 GO:0043123 GO:0046872 GO:0051091 GO:0051092

Consensus prediction of GO terms

Molecular Function	GO:0016772	GO:0005524
GO-Score	0.50	0.34
Biological Processes	GO:0006796	GO:0006743	GO:1901663
GO-Score	0.50	0.50	0.50
Cellular Component	GO:0043231	GO:0044444	GO:0031224
GO-Score	0.50	0.50	0.40

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.