I-TASSER results

I-TASSER results for job id Rv0587

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (265 residues)
MTTHAVIITYLRDQTQPAVDAIGGFYRTCVLTGKALVRRPFHWREAIEQGWFITSVSLLP
TLAVSIPLTVLIIFTLNILLAEFGAADISGAGAALGAVTQLGPLTTVLVIAGAGATAICA
DLGARTIREEIDAMEVLGIDPIHRLVVPRVVAATIVAALLNGAVITIGLVGGFVFSVFIQ
HVSAGAYVGTLTLVTGLPEVIISVVKSATFGLIAGLVGCYRGLTTKGGPKGVGTAVNETL
VLCVIALFATNVVLTTIGVRFGTGH

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MTTHAVIITYLRDQTQPAVDAIGGFYRTCVLTGKALVRRPFHWREAIEQGWFITSVSLLPTLAVSIPLTVLIIFTLNILLAEFGAADISGAGAALGAVTQLGPLTTVLVIAGAGATAICADLGARTIREEIDAMEVLGIDPIHRLVVPRVVAATIVAALLNGAVITIGLVGGFVFSVFIQHVSAGAYVGTLTLVTGLPEVIISVVKSATFGLIAGLVGCYRGLTTKGGPKGVGTAVNETLVLCVIALFATNVVLTTIGVRFGTGH
Prediction	CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
Conf.Score	9875589999999999999999999999999999985698758999999999988899999999999999999999999999896788999999999999999999999998765699999997556668999999899964456889999999999999999999999999999999849999999999999746789999999999999999999976796767984667889889999999999999999999999977899
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MTTHAVIITYLRDQTQPAVDAIGGFYRTCVLTGKALVRRPFHWREAIEQGWFITSVSLLPTLAVSIPLTVLIIFTLNILLAEFGAADISGAGAALGAVTQLGPLTTVLVIAGAGATAICADLGARTIREEIDAMEVLGIDPIHRLVVPRVVAATIVAALLNGAVITIGLVGGFVFSVFIQHVSAGAYVGTLTLVTGLPEVIISVVKSATFGLIAGLVGCYRGLTTKGGPKGVGTAVNETLVLCVIALFATNVVLTTIGVRFGTGH
Prediction	7533430152025202400420131121123112111333330220030013003300210233323312110310132034231231003000301010311330010000210020001002232453130043141330320000213012202331133033113301212111224031120131024114331133033113301200020001110414432530151022000310021333133213203324348
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MTTHAVIITYLRDQTQPAVDAIGGFYRTCVLTGKALVRRPFHWREAIEQGWFITSVSLLPTLAVSIPLTVLIIFTLNILLAEFGAADISGAGAALGAVTQLGPLTTVLVIAGAGATAICADLGARTIREEIDAMEVLGIDPIHRLVVPRVVAATIVAALLNGAVITIGLVGGFVFSVFIQHVSAGAYVGTLTLVTGLPEVIISVVKSATFGLIAGLVGCYRGLTTKGGPKGVGTAVNETLVLCVIALFATNVVLTTIGVRFGTGH

4bwzA2

0.13

0.10

0.74

0.95

wPPAS

-----------------------------------------RLKDILAV-----GKEAFLVAVLGVALPFLGGYLYGLEI----FETLPALFLGTALVTSVGITARVLQELGVLSRPYSRIILGAAVIDDVLGLIVLAC--VNGVAETGASPVVLVAGTVVTVIAILGKVLGGFLGALTQG------------VRSALTVGCGMAPRGEVGLIVAALGLKAGA----NEEEYAIVLFMVVFTTLFAPFALKPLIAWTERERAAKE

4ke2A

0.13

0.09

0.68

1.27

dPPAS2

SKAAVTAADAAAAAATIAASAASVAAATAADDAAASIATINAASAAAKSIAAAAAMAAKDTAAAAASAAAAAVASAAKALETINVKAAYAAATTANTAAAAAAATATTAAAAAAAKATIDNAAAAKAAAVATAVSDAAA---TAATAAAVAAATLEAAAAKAAATAVSAAAAAAAAAIAFAAAP---------------------------------------------------------------------------------

4bwzA2

0.11

0.09

0.77

1.06

PPAS

-----------------------------------------RLKDILAV-----GKEAFLVAVLGVALPFLGGYLYGLEI----FETLPALFLGTALVTSVGITARVLQELGVLSRPYSRIILGAAVIDDVLGLIVL-----ACVNGVAETGQELSALASPVVLVAGTVVTVIAILGKVL---GGFLGALTQGVRSALTVGCGMAPRGEVGLIVAALGLKAGA---VNEEEYAIVLFMVVFTTLFAPFALKPLIAWTERERAAKE

5cwbA

0.13

0.09

0.69

1.11

dPPAS2

ARRVAEKVERLKRSGTSEDEIAEEVAREISEVIRTLKESGSSYEVICECVARIVAEIVEALKRSEDEIAEIVARVISEVIRTLKESGSSYEVICECVARIVAEIVEALKRSGTS-EDEIAEIVARVISEVIRTLKESGSSYVQRIVEEIVEALKRSGTSEDEINEIVRRVKSEVERTLKESGS----------------------------------------------------------------------------------

3wdoA2

0.11

0.08

0.68

1.10

dPPAS2

SGMVKGSFSKVLAEPRLLKLNFGIMCLHMLLMSTFVA-----LPGQLADAG-FPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKES----------PAGY----KGTAMGVYSTSQFLGVAIGGSLGGWIDGMF-----------------DGQGVFLAGAMLAAVWLAVAS-------------------------------------------------

4bwzA2

0.15

0.11

0.75

1.08

dPPAS2

-----------------------------------------RLKDILAV--------GKEAFLVAVLGVALPFLGGYLYGLEIGFETLPALFLGTALVTSVGITARVLQELGVLSRPYSRIILGAAVIDDVLGLIVLAC--VNGVAETGASPVVLVAGTVVTVIAILGKVLGGFLGALTQGVRSALTVGCGMAP------------RGEVGLIVAALGLKAGA---VNEEEYAIVLFMVVFTTLFAPFALKPLIAWTERERAAKE

4yxxA

0.13

0.09

0.72

1.04

dPPAS2

ALKILKVAAELGTTVEEAVKRALKLKTKLGVSLEQAL----KILEVAAELGTTVEEAVKRALKLKTKLGLEQALKILEVAAKLGTTVEEAVKRALKLKTKLGSLEQALKILKVAAELGTT--VEEAVKRALKLKTKLGVSLLKILEVAAELVEEAVKRALKLKTKLLEQALKILEVAAKLGTTVEEAVKRALKLKTK--------------------------------------------------------------------

4rp8A2

0.13

0.12

0.98

0.90

MUSTER

ISDTYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTIICTAILVSLY--WGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLKKEEIDCAAIYSFAPVVWGFMWGTIGQLIAVGILVACGSSILIIPG-FIPMFFSNATIGVFANHFGGW--RAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRA

4ke2A

0.10

0.08

0.74

0.89

dPPAS

MNIDPAARAAAAAAASKAAVTAADAAAAAATIAASAASVAAATAADDAAASIATINAASAAAKSIAAAAAMAAKDTAAAAASAAAAAVASAAKALETINVKAAYAAATTANTAAAAAAATATTAAAAAAAKATIDNAAAAKAAAVATAVSDAAATAATAAAVAAATLEAAAAKAAATAVSAAAAAAAAAIAFAAAP---------------------------------------------------------------------

4bwzA2

0.14

0.10

0.74

wdPPAS

-----------------------------------------RLKDILA------GKEAFLVAVLGVALPFLGGYLYGLEI----FETLPALFLGTALVTSVGITARVLQELGVLSRPYSRIILGAAVIDDVLGLIVLAC--VNGVAET-ASPVVLVAGTVVTVIAILGKVLGGFLGALTQGVRSALTVGCGMAP------------RGEVGLIVAALGLKAGA----NEEEYAIVLFMVVFTTLFAPFALKPLIAWTERERAAKE

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-3.68

Estimated TM-score = 0.31±0.10

Estimated RMSD = 14.9±3.6Å

Download Model 2

C-score=-4.09

Download Model 3

C-score=-3.91

Download Model 4

C-score=-3.87

Download Model 5

C-score=-4.74

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4bwzA	0.726	3.51	0.124	0.875
2	4czbA	0.637	4.42	0.086	0.868
3	4cz8A	0.636	4.03	0.090	0.815
4	1zcdA	0.612	4.27	0.086	0.796
5	4n7wA	0.612	4.47	0.068	0.845
6	3zuxA	0.612	4.42	0.077	0.838
7	5dgqA	0.457	5.96	0.048	0.766
8	5a1sA	0.450	5.09	0.106	0.664
9	3h7lA	0.450	5.77	0.048	0.740
10	4a01A	0.447	5.41	0.056	0.702

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.05	3	2nwwB	TB1	Rep, Mult	71,92,161,164,165,168
2	0.05	3	4z3bA	MN	Rep, Mult	215,232,236
3	0.03	2	5sy1A	CLR	Rep, Mult	247,250
4	0.03	2	3v2d2	MG	Rep, Mult	145,149
5	0.03	2	2axtl	UNK	Rep, Mult	244,247
6	0.02	1	2x7qA	CA	Rep, Mult	123,129
7	0.02	1	3kcuA	MA5	Rep, Mult	102,120,121,123,124,127
8	0.02	1	2o014	CLA	Rep, Mult	237,240
9	0.02	1	2o01H	CLA	Rep, Mult	112,122
10	0.02	1	1jb0K	CLA	Rep, Mult	104,109
11	0.02	1	4czaA	TL	Rep, Mult	37,99,126,130
12	0.02	1	3nmvB	MG	Rep, Mult	130,168
13	0.02	1	1fipA	III	Rep, Mult	67,68
14	0.02	1	1nquA	RDL	Rep, Mult	209,210
15	0.02	1	3wmm7	CRT	Rep, Mult	58,68
16	0.02	1	3mmhB	MG	Rep, Mult	103,104
17	0.02	1	1u7gA	NH4	Rep, Mult	108,122,203
18	0.02	1	3zuyA	PTY	Rep, Mult	67,71,74,86,94,136,139
19	0.02	1	1wuuB	MG	Rep, Mult	65,132
20	0.02	1	3zuxA	PTY	Rep, Mult	162,166,170,241,245,248,249,252

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2zr3B	0.403	6.20	0.063	0.668	6.1.1.11	199
2	0.060	2j2fA	0.427	5.67	0.059	0.683	1.14.19.2,1.14.99.6	NA
3	0.060	3fg3A	0.438	6.10	0.099	0.740	4.2.1.92,1.13.11.40	NA
4	0.060	1sesA	0.409	6.08	0.049	0.668	6.1.1.11	NA
5	0.060	3dy5A	0.399	6.16	0.047	0.683	1.13.11.40,4.2.1.92	NA
6	0.060	1i7qA	0.394	5.85	0.071	0.630	4.1.3.27	NA
7	0.060	1mhyD	0.395	5.98	0.056	0.660	1.14.13.25	NA
8	0.060	1m56A	0.395	5.96	0.049	0.645	1.9.3.1	NA
9	0.060	1serB	0.269	6.72	0.031	0.494	6.1.1.11	NA
10	0.060	1ayxA	0.398	5.85	0.055	0.653	3.2.1.3	135
11	0.060	1gaiA	0.401	5.56	0.047	0.623	3.2.1.3	135
12	0.060	3errA	0.406	6.01	0.045	0.653	6.1.1.11	NA
13	0.060	1fziA	0.394	5.91	0.052	0.664	1.14.13.25	NA
14	0.060	1z1lA	0.385	5.41	0.030	0.585	3.1.4.17	163
15	0.060	3d3lA	0.407	5.97	0.066	0.687	1.13.11.31	NA
16	0.060	1wleB	0.410	5.76	0.036	0.649	6.1.1.11	NA
17	0.060	1j0mA	0.341	6.27	0.044	0.596	4.2.2.12	200
18	0.060	1hxgA	0.395	5.62	0.083	0.626	4.2.3.9,4.1.99.7	105,106
19	0.060	2dq3A	0.399	6.15	0.055	0.657	6.1.1.11	112

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.14	0.726	3.51	0.12	0.88	4bwzA	GO:0006810 GO:0006812 GO:0015299 GO:0016020 GO:0016021 GO:0046872 GO:0055085 GO:1902600
1	0.09	0.672	3.72	0.06	0.84	4czbB	GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006814 GO:0015297 GO:0015299 GO:0016020 GO:0016021 GO:0042802 GO:0055085 GO:1902600
2	0.08	0.390	5.62	0.05	0.61	4atvA	GO:0005886 GO:0006810 GO:0006811 GO:0006814 GO:0006885 GO:0015081 GO:0015297 GO:0015491 GO:0015992 GO:0016020 GO:0016021 GO:0035725 GO:0055085 GO:0098655
3	0.08	0.645	4.07	0.09	0.83	4cz8A	GO:0006810 GO:0006812 GO:0015299 GO:0016020 GO:0016021 GO:0055085 GO:1902600
4	0.07	0.612	4.47	0.07	0.85	4n7wA	GO:0016020 GO:0016021
5	0.07	0.612	4.42	0.08	0.84	3zuxA	GO:0016020 GO:0016021
6	0.06	0.457	5.96	0.05	0.77	5dgqA	GO:0003824 GO:0004553 GO:0005975
7	0.06	0.401	5.06	0.09	0.60	3x17A	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0046872
8	0.06	0.450	5.77	0.05	0.74	3h7lA	GO:0003824 GO:0004553 GO:0005975 GO:0008810
9	0.06	0.324	5.79	0.04	0.52	1g87B	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0030246 GO:0030248
10	0.06	0.292	6.52	0.03	0.51	1ia7A	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0046872
11	0.06	0.317	6.65	0.04	0.58	3gzkA	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0046872
12	0.06	0.293	6.25	0.04	0.51	4rpfA	GO:0000166 GO:0004413 GO:0005524 GO:0005737 GO:0006566 GO:0008652 GO:0009088 GO:0016301 GO:0016310 GO:0016740
13	0.06	0.405	5.00	0.06	0.61	4cj1A	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245
14	0.06	0.273	6.06	0.06	0.46	4w65A	GO:0004553 GO:0005975 GO:0016787 GO:0046872
15	0.06	0.323	6.05	0.05	0.55	1u0uA	GO:0003824 GO:0005737 GO:0008152 GO:0009058 GO:0016740 GO:0016746 GO:0016747 GO:0050198
16	0.06	0.324	5.14	0.06	0.48	4b2tb	GO:0000166 GO:0002199 GO:0005524 GO:0005737 GO:0005832 GO:0005874 GO:0006457 GO:0007339 GO:0031625 GO:0043209 GO:0044297 GO:0051082 GO:0051131 GO:0070062 GO:1901998
17	0.06	0.266	5.88	0.03	0.44	3rq0A	GO:0004553 GO:0005975 GO:0016787 GO:0046872
18	0.06	0.247	5.94	0.06	0.42	3r0rA	GO:0019069 GO:0042025

Consensus prediction of GO terms

Molecular Function	GO:0015298	GO:0015078
GO-Score	0.56	0.56
Biological Processes	GO:0098662	GO:0015992	GO:0030001
GO-Score	0.56	0.56	0.33
Cellular Component	GO:0016021	GO:0071944
GO-Score	0.39	0.33

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.