I-TASSER results

I-TASSER results for job id Rv0577

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (261 residues)
MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNPVPGGGGVYSMATLNGEAVA
AIAPMPPGAPEGMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPT
GAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSMEIAAGQNY
RVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGR
FAVLSDPQGAIFSVLKPAPQQ

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNPVPGGGGVYSMATLNGEAVAAIAPMPPGAPEGMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSMEIAAGQNYRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKPAPQQ
Prediction	CCCCCCCCCCCEEEEEEHCCCHHHHHHHHHHHHCCEEEHCCCCCCCCCEEEEHCCCCCEEEEEHCCCCCCCCCCCEEEEEEEHCCHHHHHHHHHHHCCEEEHCCEHCCCCEEEEEEHCCCCCEEEEEHCCCCCCCCCCCCCCCCCEEEEHCCCHHHHHHHHHHHHCCEEEHCCCCCCCEEEEEHCCCCCEEEHCCCCCCCCCCEEEEEEEHCCHHHHHHHHHHHCCEEEHCCEHCCCCEEEEEEHCCCCCEEEEEHCCCCC
Conf.Score	999888999965799976798999999999997978987789888875799976997578887578888888997689999857899999999999999986875658985899999799988999985788877777788876567887699999999999998998876688998758998579975677647898889985899999699999999999999989858755689858999987999889999868999
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNPVPGGGGVYSMATLNGEAVAAIAPMPPGAPEGMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSMEIAAGQNYRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKPAPQQ
Prediction	846446344120000012153042014124520403144343642322001011644200000223443465321000010105303400520372404123222621420100000023201000001132343433334432331202032053025102501413155262442000000224432000003335743211000001042034005203725141233325223100000012234020001124578
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCEEEEEEHCCCHHHHHHHHHHHHCCEEEHCCCCCCCCCEEEEHCCCCCEEEEEHCCCCCCCCCCCEEEEEEEHCCHHHHHHHHHHHCCEEEHCCEHCCCCEEEEEEHCCCCCEEEEEHCCCCCCCCCCCCCCCCCEEEEHCCCHHHHHHHHHHHHCCEEEHCCCCCCCEEEEEHCCCCCEEEHCCCCCCCCCCEEEEEEEHCCHHHHHHHHHHHCCEEEHCCEHCCCCEEEEEEHCCCCCEEEEEHCCCCC
MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNPVPGGGGVYSMATLNGEAVAAIAPMPPGAPEGMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSMEIAAGQNYRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKPAPQQ

3oxhA

0.99

0.92

0.93

3.09

MUSTER

MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNP-----GVYSMATLNGEAVAAIAPMPP---GAMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSM-------YRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKAA---

3oxhA

0.99

0.92

0.93

4.35

dPPAS

MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNP-----GVYSMATLNGEAVAAIAPMPPG---AMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSM-------YRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKAA---

3oxhA

0.98

0.91

0.93

4.74

wdPPAS

MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNPGV-----YSMATLNGEAVAAIAPMPP---GAMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSM-------YRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKAA---

3oxhA

0.98

0.92

0.93

3.83

wMUSTER

MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNPG-----VYSMATLNGEAVAAIAPMPP---GAMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSM-------YRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKAA---

3oxhA

0.98

0.91

0.93

4.96

wPPAS

MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNPGV-----YSMATLNGEAVAAIAPMPP---GAMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSM-------YRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKAA---

3oxhA

0.99

0.92

0.93

8.78

dPPAS2

MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNP-----GVYSMATLNGEAVAAIAPMPPG---AMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSM-------YRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKAA---

3oxhA

0.98

0.91

0.93

4.53

PPAS

MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNPGV-----YSMATLNGEAVAAIAPMPP---GAMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSM-------YRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKAA---

3oxhA

0.99

0.92

0.93

6.05

Env-PPAS

MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNP-----GVYSMATLNGEAVAAIAPMPP---GAMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSM-------YRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKAA---

4ro6A

0.16

0.97

1.69

MUSTER

VDWHKHDSK-RMLHAVYRVGDLDRTIKYYTECFGMLLRKRDVPDEKYTNAFLGFNTNFAVELTYNYGVDKYDIGTFGHFAIANDDVYKLAENIKSKGGKITREPGPVKGSTVIAFAQDPDGYMFELIQR--------ADTPEPLCQVMLRVGDLERSIKFYEKALGMKLLRKKDVPKYTIAMLGYADEDKTTVLVTEYSKGNAYAQVAIGTNDVYKSAEAVDLATGKILRQPGPLPGNTKIASFVDPDGWKVVLVDNTDFL

4ro6A

0.17

0.16

0.97

2.11

dPPAS

-VDWHKHDSKRMLHAVYRVGDLDRTIKYYTECFGMKLLRKRDVPDEKYTNAFLGFGPEVELTYNYGVDKYDIGTGFGHFAIANDDVYKLAENIKSKGGKITREPGPVKGGSVIAFAQDPDGYMFELIQRADTPEP--------LCQVMLRVGDLERSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADTYNYGVTEYSKGNAYAQVAIGTNDAEAVDLATKELGGKILRQPGPLPGINKIASFVDPDGWKVVLVDNTDFL

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 3 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.51

Estimated TM-score = 0.92±0.06

Estimated RMSD = 2.9±2.1Å

Download Model 2

C-score=-1.37

Download Model 3

C-score=-1.37

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1eiqA	0.651	4.23	0.121	0.904
2	1kmyA	0.650	4.21	0.134	0.900
3	1uz4A	0.427	5.96	0.035	0.701
4	1uhvA	0.422	5.93	0.044	0.694
5	1gonA	0.419	5.95	0.056	0.678
6	1aldA	0.413	6.20	0.070	0.694
7	1nthA	0.413	5.93	0.057	0.674
8	1qhoA	0.413	6.19	0.044	0.709
9	1fa6A	0.406	2.50	0.180	0.460
10	1h5vA	0.396	5.71	0.066	0.640

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.20	2	3oxhA	PMB	Rep, Mult	40,49,107,110,132
2	0.09	1	3oxhA	XYL	Rep, Mult	139,145,146,190
3	0.06	1	1f9z0	III	Rep, Mult	7,15,16,17,18,61,205,206,207,208,209,210,212,250,253,255,257
4	0.06	1	2quvA	PO4	Rep, Mult	83,84,85,112
5	0.06	1	2quvB	PO4	Rep, Mult	119,120,121,157
6	0.06	1	1lkdA	BP6	Rep, Mult	207,250,251,253
7	0.06	1	1zalB	PO4	Rep, Mult	120,121,152,153,154,156

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.075	1kmyA	0.650	4.21	0.134	0.900	1.13.11.39	NA
2	0.060	1didA	0.391	6.32	0.068	0.678	5.3.1.5	NA
3	0.060	1gnxA	0.418	5.76	0.071	0.670	3.2.1.21	NA
4	0.060	1e5jA	0.396	5.73	0.066	0.640	3.2.1.4	161
5	0.060	1fbaC	0.405	6.00	0.059	0.667	4.1.2.13	NA
6	0.060	1lpsA	0.376	6.29	0.054	0.667	3.1.1.3	NA
7	0.060	1px8A	0.421	5.90	0.044	0.690	3.2.1.37	NA
8	0.060	1oc5A	0.382	5.71	0.049	0.625	3.2.1.91	NA
9	0.060	1bwkA	0.392	5.99	0.073	0.647	1.6.99.1	NA
10	0.060	1htiA	0.375	5.99	0.033	0.632	5.3.1.1	NA
11	0.060	2madH	0.381	6.83	0.064	0.728	1.4.99.3	119
12	0.060	1qw6A	0.396	5.85	0.063	0.659	1.14.13.39	NA
13	0.060	1xc6A	0.413	6.55	0.051	0.755	3.2.1.23	NA
14	0.060	1f9zA	0.406	2.49	0.180	0.460	4.4.1.5	NA
15	0.060	1d0cA	0.397	5.93	0.086	0.659	1.14.13.39	NA
16	0.060	1xfbA	0.419	5.77	0.045	0.670	4.1.2.13	NA
17	0.060	1g5aA	0.377	6.26	0.054	0.659	2.4.1.4	148
18	0.060	1fdjA	0.375	6.25	0.079	0.651	4.1.2.13	NA
19	0.060	1s46A	0.365	6.37	0.051	0.659	2.4.1.4	85
20	0.060	3bllA	0.386	6.33	0.069	0.670	2.4.2.29	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.33	0.656	4.18	0.11	0.90	2ehzA	GO:0003824 GO:0006725 GO:0008198 GO:0016491 GO:0016702 GO:0019439 GO:0046872 GO:0051213 GO:0055114 GO:1901170
1	0.19	0.650	4.21	0.13	0.90	1kmyA	GO:0003824 GO:0005506 GO:0006725 GO:0008198 GO:0016491 GO:0018583 GO:0019439 GO:0042178 GO:0046872 GO:0051213 GO:0055114
2	0.17	0.676	4.22	0.12	0.92	3hpvA	GO:0003824 GO:0006725 GO:0008198 GO:0016491 GO:0018577 GO:0019439 GO:0046872 GO:0051213 GO:0055114
3	0.15	0.651	4.24	0.12	0.90	1eilA	GO:0003824 GO:0005506 GO:0006725 GO:0008198 GO:0016491 GO:0018583 GO:0019439 GO:0042178 GO:0046872 GO:0051213 GO:0055114
4	0.15	0.670	4.03	0.16	0.91	3b59A	GO:0046872 GO:0051213 GO:0055114
5	0.14	0.654	4.29	0.10	0.91	2wl9A	GO:0003824 GO:0005506 GO:0006725 GO:0008198 GO:0016491 GO:0019439 GO:0042178 GO:0046872 GO:0051213 GO:0055114
6	0.13	0.641	4.35	0.12	0.90	2zi8A	GO:0003824 GO:0005506 GO:0006629 GO:0006707 GO:0006725 GO:0008198 GO:0008202 GO:0008203 GO:0009405 GO:0016042 GO:0016491 GO:0019439 GO:0046872 GO:0047071 GO:0051213 GO:0055114 GO:0070723
7	0.12	0.676	4.07	0.10	0.91	3lm4D	GO:0016491 GO:0018577 GO:0046872 GO:0051213 GO:0055114
8	0.10	0.653	4.25	0.12	0.92	3vb0A	GO:0003824 GO:0006725 GO:0008198 GO:0016491 GO:0019439 GO:0046872 GO:0051213 GO:0055114
9	0.10	0.675	3.98	0.14	0.90	1f1uA	GO:0046872 GO:0051213 GO:0055114
10	0.10	0.676	3.90	0.12	0.90	4ghcC	GO:0046872 GO:0051213 GO:0055114
11	0.10	0.387	3.35	0.17	0.47	4hc5D	GO:0051213 GO:0055114
12	0.06	0.369	2.82	0.17	0.44	1lqkA	GO:0004364 GO:0005737 GO:0016740 GO:0046677 GO:0046872
13	0.06	0.364	3.56	0.11	0.46	3hnqA	GO:0009405
14	0.06	0.383	3.25	0.16	0.46	3rmuA	GO:0004493 GO:0005739 GO:0005759 GO:0016853 GO:0019626 GO:0046491 GO:0046872
15	0.06	0.367	3.41	0.15	0.46	1npbA	GO:0004364 GO:0005737 GO:0016740 GO:0046677 GO:0046872
16	0.06	0.353	7.18	0.03	0.71	1gjqA	GO:0009055 GO:0016491 GO:0020037 GO:0042597 GO:0046872 GO:0050418 GO:0050421 GO:0055114
17	0.06	0.350	6.54	0.07	0.64	4wghA	GO:0016491 GO:0055114
18	0.06	0.354	7.21	0.07	0.71	1aomB	GO:0009055 GO:0016491 GO:0020037 GO:0042597 GO:0046872 GO:0050418 GO:0050421 GO:0055114
19	0.06	0.293	6.45	0.09	0.52	2rb9D

Consensus prediction of GO terms

Molecular Function	GO:0008198	GO:0019114	GO:0018583
GO-Score	0.62	0.34	0.31
Biological Processes	GO:0055114	GO:1901170	GO:0042178
GO-Score	0.67	0.33	0.31
Cellular Component
GO-Score

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.