I-TASSER results

I-TASSER results for job id Rv0518

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (231 residues)
MSRPGTYVIGLTLLVGLVVGNPGCPRSYRPLTLDYRLNPVAVIGDSYTTGTDEGGLGSKS
WTARTWQMLAARGVRIAADVAAEGRAGYGVPGDHGNVFEDLTARAVQPDDALVVFFGSRN
DQGMDPEDPEMLAEKVRDTFDLARHRAPSASLLVIAPPWPTADVPGPMLRIRDVLGAQAR
AAGAVFVDPIADHWFVDRPELIGADGVHPNDAGHEYLADKIAPLISMELVG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MSRPGTYVIGLTLLVGLVVGNPGCPRSYRPLTLDYRLNPVAVIGDSYTTGTDEGGLGSKSWTARTWQMLAARGVRIAADVAAEGRAGYGVPGDHGNVFEDLTARAVQPDDALVVFFGSRNDQGMDPEDPEMLAEKVRDTFDLARHRAPSASLLVIAPPWPTADVPGPMLRIRDVLGAQARAAGAVFVDPIADHWFVDRPELIGADGVHPNDAGHEYLADKIAPLISMELVG
Prediction	CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEHCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
Conf.Score	976999999999999998667877665544456788878999868855787899998788799999999987888679998756677778898765789999998755998999988866788999999999999999999999989999599987999998567999999999999998879958767555766788566578999999999999999999999998589
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MSRPGTYVIGLTLLVGLVVGNPGCPRSYRPLTLDYRLNPVAVIGDSYTTGTDEGGLGSKSWTARTWQMLAARGVRIAADVAAEGRAGYGVPGDHGNVFEDLTARAVQPDDALVVFFGSRNDQGMDPEDPEMLAEKVRDTFDLARHRAPSASLLVIAPPWPTADVPGPMLRIRDVLGAQARAAGAVFVDPIADHWFVDRPELIGADGVHPNDAGHEYLADKIAPLISMELVG
Prediction	532100010131111000112234345344243645242100213021202214346441003101520475426130200031221222443314213500440253434032120304313244231530263244004202730570200000001024523520430151026107624020000003332433430102220011453042405331513464168
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEHCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
MSRPGTYVIGLTLLVGLVVGNPGCPRSYRPLTLDYRLNPVAVIGDSYTTGTDEGGLGSKSWTARTWQMLAARGVRIAADVAAEGRAGYGVPGDHGNVFEDLTARAVQPDDALVVFFGSRNDQGMDPEDPEMLAEKVRDTFDLARHRAPSASLLVIAPPWPTADVPGPMLRIRDVLGAQARAAGAVFVDPIADHWFVDRPELIGADGVHPNDAGHEYLADKIAPLISMELVG

4jggA

0.23

0.18

0.78

2.19

MUSTER

-------------------------------------QTLLVVGDSISAALGL--DTSQGWVALLQKRLADEGYD--YRVVNASISGDTSA----GGLARLPALLAEEKPALVVIELGGNDG-LRGMAPAQLQQNLASMAQKARAE--GAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVP-FFLEGVGGVQGMMQADGIHPALAAQPRLLENVWPTLKPLL--

4jggA

0.23

0.18

0.78

2.41

dPPAS

-------------------------------------QTLLVVGDSISAALGL--DTSQGWVALLQKRLADEGYDY--RVVNASISGDTSA----GGLARLPALLAEEKPALVVIELGGNDG-LRGMAPAQLQQNLASMAQKARAE--GAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVP-FFLEGVGGVQGMMQADGIHPALAAQPRLLENVWPTLKPLL--

4jggA

0.23

0.18

0.78

2.97

wdPPAS

-------------------------------------QTLLVVGDSISAALGL--DTSQGWVALLQKRLADEGYD--YRVVNASISGDTS----AGGLARLPALLAEEKPALVVIELGGNDG-LRGMAPAQLQQNLASMAQKARAEG--AKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLEG-VGGVQGMMQADGIHPALAAQPRLLENVWPTLKPLL--

4jggA

0.23

0.18

0.77

2.65

wMUSTER

--------------------------------------TLLVVGDSISAALGL--DTSQGWVALLQKRLADEGYD--YRVVNASISGDTSA----GGLARLPALLAEEKPALVVIELGGNDG-LRGMAPAQLQQNLASMAQKARAE--GAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVP-FFLEGVGGVQGMMQADGIHPALAAQPRLLENVWPTLKPLL--

4jggA

0.23

0.18

0.78

3.24

wPPAS

-------------------------------------QTLLVVGDSISAALGL--DTSQGWVALLQKRLADEGYD--YRVVNASISGDTS----AGGLARLPALLAEEKPALVVIELGGNDG-LRGMAPAQLQQNLASMAQKARAEG--AKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLEG-VGGVQGMMQADGIHPALAAQPRLLENVWPTLKPLL--

4jggA

0.23

0.18

0.78

4.90

dPPAS2

-------------------------------------QTLLVVGDSISAALGL--DTSQGWVALLQKRLADEGYDY--RVVNASISGDTSA----GGLARLPALLAEEKPALVVIELGGNDG-LRGMAPAQLQQNLASMAQKARAE--GAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVP-FFLEGVGGVQGMMQADGIHPALAAQPRLLENVWPTLKPLL--

4jggA

0.23

0.18

0.78

2.83

PPAS

-------------------------------------QTLLVVGDSISAALGL--DTSQGWVALLQKRLADEGYD--YRVVNASISGDTSA----GGLARLPALLAEEKPALVVIELGGNDG-LRGMAPAQLQQNLASMAQKARAEG--AKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLE-GVGGVQGMMQADGIHPALAAQPRLLENVWPTLKPLL--

4jggA

0.23

0.18

0.78

3.58

Env-PPAS

-------------------------------------QTLLVVGDSISAALGL--DTSQGWVALLQKRLADEGYDY--RVVNASISGDTSA----GGLARLPALLAEEKPALVVIELGGNDG-LRGMAPAQLQQNLASMAQKARAEG--AKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLE-GVGGVQGMMQADGIHPALAAQPRLLENVWPTLKPLL--

3hp4A

0.14

0.11

0.79

2.02

MUSTER

------------------------------------DNTILILGD-LSAAYGL--QQEEGWVKLLQDKYDAEQSD--IVLINASISGETS----GGALRRLDALLEQYEPTHVLIELGANDG-LRGFPVKKMQTNLTALVKKSQAA--NAMTALMEIYIPPNYGPRYSKMFTSSFTQISEDTNAHLMNFFMLD-IAGKSDLMQNDSLHPNKKAQPLIRDEMYDSIKKWLNN

1ivnA

0.17

0.13

0.77

2.17

dPPAS

------------------------------------ADTLLILGDSLSAGYR--MSASAAWPALLNDKWS------KTSVVNASISGDTSQ----QGLARLPALLKQHQPRWVLVELGGNDG-LRGFQPQQTEQTLRQILQDVKAA--NAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVPLLPFFMEE-VYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVN-

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-0.91

Estimated TM-score = 0.60±0.14

Estimated RMSD = 7.6±4.3Å

Download Model 2

C-score=-1.69

Download Model 3

C-score=-2.41

Download Model 4

C-score=-3.17

Download Model 5

C-score=-1.79

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4jggA	0.740	1.35	0.233	0.779
2	4k3uA	0.719	3.42	0.192	0.892
3	3hp4A	0.711	2.05	0.137	0.788
4	1escA	0.697	2.69	0.222	0.818
5	1ivnA	0.695	2.00	0.169	0.771
6	5jd3A	0.692	2.90	0.176	0.814
7	4q9aA	0.690	2.97	0.152	0.818
8	1yzfA	0.687	2.64	0.195	0.796
9	3x0hA	0.687	2.39	0.182	0.783
10	3w7vA	0.685	2.79	0.181	0.805

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.35	9	3dt9A	GD7	Rep, Mult	45,46,86,87,119,120,207,208
2	0.23	6	1u8uA	OCA	Rep, Mult	45,46,87,119,120,158,160,208
3	0.05	2	1v2gA	IMD	Rep, Mult	69,77,78,226,229
4	0.03	1	3dc7B	NA	Rep, Mult	44,45,47,48,116
5	0.02	1	3i26D	UUU	Rep, Mult	161,163,173,176,186,188
6	0.02	1	3x0hA	BOG	Rep, Mult	50,120,203,208
7	0.02	1	1v2gA	IMD	Rep, Mult	66,80,222,225

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.317	2q0sH	0.680	2.62	0.215	0.783	3.1.1.2	83,120,208
2	0.118	1ivnA	0.695	2.00	0.169	0.771	3.1.1.5,	46,87,120,205,208
3	0.114	1uqrK	0.450	3.72	0.088	0.593	4.2.1.10	NA
4	0.067	1h0rA	0.458	3.34	0.082	0.567	4.2.1.10	NA
5	0.066	1gqoB	0.452	3.39	0.075	0.567	4.2.1.10	NA
6	0.066	1d0iH	0.472	3.66	0.077	0.615	4.2.1.10	NA
7	0.066	2uygL	0.456	3.19	0.121	0.563	4.2.1.10	NA
8	0.066	2uygA	0.456	3.29	0.121	0.563	4.2.1.10	NA
9	0.066	2c4wA	0.481	3.51	0.074	0.619	4.2.1.10	120,198
10	0.066	3lwzA	0.459	3.37	0.059	0.584	4.2.1.10	NA
11	0.066	1uqrD	0.465	3.23	0.081	0.584	4.2.1.10	NA
12	0.060	2pd6D	0.551	4.13	0.103	0.740	1.1.1.62,1.1.1.63	NA
13	0.060	1h5qF	0.550	4.17	0.067	0.740	1.1.1.138	44
14	0.060	1pp4A	0.628	3.36	0.167	0.771	3.1.1.-	87
15	0.060	1flcC	0.563	4.00	0.090	0.736	3.1.1.53	183,231
16	0.060	2b4qA	0.551	4.02	0.074	0.732	1.1.1.100	NA
17	0.060	3gr6D	0.560	4.06	0.077	0.749	1.3.1.9	NA
18	0.060	1vyhN	0.642	2.46	0.137	0.740	3.1.1.47	120
19	0.060	1vyhA	0.642	2.45	0.137	0.740	3.1.1.47	46,87,120,205,208
20	0.060	1deoA	0.627	3.36	0.167	0.771	3.1.1.-	46,87,120,205,208
21	0.060	1bwpA	0.637	2.55	0.128	0.736	3.1.1.47	46,87,120,205,208
22	0.060	2vz8B	0.562	4.49	0.081	0.792	2.3.1.85	116
23	0.060	2jfdA	0.366	6.13	0.065	0.654	2.3.1.85	101
24	0.060	1uzmB	0.539	3.92	0.092	0.719	1.1.1.100	NA
25	0.060	1uznA	0.554	3.90	0.091	0.736	1.1.1.100	NA
26	0.060	1gegE	0.560	3.84	0.099	0.732	1.1.1.5	NA
27	0.060	2vz8A	0.549	4.39	0.079	0.762	2.3.1.85	116
28	0.060	3kvnX	0.653	3.19	0.174	0.801	3.1.1.1	207,209
29	0.060	1escA	0.697	2.69	0.222	0.818	3.1.1.-	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.37	0.695	2.00	0.17	0.77	1ivnA	GO:0004620 GO:0004622 GO:0006508 GO:0006629 GO:0008233 GO:0016290 GO:0016298 GO:0016787 GO:0030288 GO:0042597 GO:0042802 GO:0047617
1	0.35	0.740	1.35	0.23	0.78	4jggA	GO:0004064 GO:0004622 GO:0005576 GO:0016787 GO:0052689
2	0.33	0.719	3.42	0.19	0.89	4k3uA	GO:0016787
3	0.31	0.652	2.27	0.17	0.74	4rshA	GO:0016020 GO:0016021
4	0.29	0.687	2.64	0.20	0.80	1yzfA	GO:0016787
5	0.29	0.676	2.79	0.21	0.80	4tx1A	GO:0004064 GO:0016787
6	0.27	0.686	2.89	0.19	0.81	4jhlA	GO:0000272 GO:0005737 GO:0005975 GO:0016787 GO:0045493 GO:0046555 GO:0052689
7	0.27	0.687	2.39	0.18	0.78	3x0hA	GO:0004553 GO:0005576 GO:0005975 GO:0030248
8	0.26	0.711	2.05	0.14	0.79	3hp4A
9	0.25	0.669	2.66	0.17	0.77	3rjtA
10	0.20	0.633	2.60	0.15	0.74	4hf7A	GO:0016787
11	0.18	0.639	2.54	0.17	0.74	4ppyA	GO:0016787
12	0.17	0.620	2.64	0.14	0.72	3p94A
13	0.13	0.574	3.05	0.08	0.69	5cd9A	GO:0005938 GO:0007275 GO:0007277 GO:0007278 GO:0007279 GO:0007281 GO:0007314 GO:0007315 GO:0007318 GO:0007359 GO:0007616 GO:0007632 GO:0008103 GO:0008542 GO:0008595 GO:0019094 GO:0030719 GO:0035282 GO:0040040 GO:0043186 GO:0043488 GO:0045495 GO:0045727 GO:0046011 GO:0046594 GO:0048477
14	0.13	0.676	2.81	0.17	0.80	1z8hA
15	0.10	0.657	3.15	0.16	0.79	3milA	GO:0006083 GO:0006629 GO:0016042 GO:0016787 GO:0016788
16	0.08	0.626	2.47	0.12	0.72	2hsjD
17	0.07	0.457	5.28	0.05	0.72	1r30B	GO:0003824 GO:0004076 GO:0005506 GO:0009102 GO:0016740 GO:0046872 GO:0051536 GO:0051537 GO:0051539
18	0.07	0.434	5.00	0.06	0.67	3e38B	GO:0003677 GO:0003824 GO:0003887 GO:0006260 GO:0046872 GO:0071897
19	0.06	0.440	4.53	0.05	0.65	2g0tB
20	0.06	0.290	6.06	0.03	0.51	3uonA	GO:0003796 GO:0003824 GO:0004871 GO:0004930 GO:0005886 GO:0005887 GO:0006940 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007197 GO:0007207 GO:0007213 GO:0007271 GO:0007399 GO:0008016 GO:0008144 GO:0008152 GO:0009253 GO:0009615 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0016907 GO:0016998 GO:0019076 GO:0019835 GO:0030054 GO:0030425 GO:0030430 GO:0032279 GO:0032280 GO:0042742 GO:0043025 GO:0043679 GO:0045202 GO:0045211
21	0.06	0.391	5.20	0.07	0.62	4axsA	GO:0006525 GO:0008804 GO:0016301 GO:0016310 GO:0016740
22	0.06	0.287	5.98	0.07	0.50	3rzeA	GO:0003796 GO:0003824 GO:0004871 GO:0004930 GO:0004969 GO:0005654 GO:0005737 GO:0005886 GO:0005887 GO:0006954 GO:0007165 GO:0007186 GO:0007200 GO:0007613 GO:0008152 GO:0008542 GO:0009253 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0016998 GO:0019076 GO:0019229 GO:0019835 GO:0030430 GO:0032962 GO:0042742 GO:0043114 GO:0045907 GO:0048016 GO:0048167 GO:0048245 GO:0050804 GO:0071420

Consensus prediction of GO terms

Molecular Function	GO:0004622	GO:0042802	GO:0016290	GO:0008233	GO:0004064
GO-Score	0.59	0.36	0.36	0.36	0.35
Biological Processes	GO:0006508	GO:0006629
GO-Score	0.36	0.36
Cellular Component	GO:0030288	GO:0005576	GO:0016021
GO-Score	0.36	0.35	0.31

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.