I-TASSER results

I-TASSER results for job id Rv0485

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (438 residues)
MYSTNRTSQSLSRKPGRKHQLRSHRYVMPPSLHLSDSAAASVFRAVRLRGPVGRDVIAGS
TSLSIATVNRQVIALLEAGLLRERADLAVSGAIGRPRVPVEVNHEPFVTLGIHIGARTTS
IVATDLFGRTLDTVETPTPRNAAGAALTSLADSADRYLQRWRRRRALWVGVTLGGAVDSA
TGHVDHPRLGWRQAPVGPVLADALGLPVSVASHVDAMAGAELMLGMRRFAPSSSTSLYVY
ARETVGYALMIGGRVHCPASGPGTIAPLPVHSEMLGGTGQLESTVSDEAVLAAARRLRII
PGIASRTRTGGSATAITDLLRVARAGNQQAKELLAERARVLGGAVALLRDLLNPDEVVVG
GQAFTEYPEAMEQVEAAFTAGSVLAPRDIRVTVFGNRVQEAGAGIVSLSGLYADPLGALR
RSGALDARLQDTAPEALA

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MYSTNRTSQSLSRKPGRKHQLRSHRYVMPPSLHLSDSAAASVFRAVRLRGPVGRDVIAGSTSLSIATVNRQVIALLEAGLLRERADLAVSGAIGRPRVPVEVNHEPFVTLGIHIGARTTSIVATDLFGRTLDTVETPTPRNAAGAALTSLADSADRYLQRWRRRRALWVGVTLGGAVDSATGHVDHPRLGWRQAPVGPVLADALGLPVSVASHVDAMAGAELMLGMRRFAPSSSTSLYVYARETVGYALMIGGRVHCPASGPGTIAPLPVHSEMLGGTGQLESTVSDEAVLAAARRLRIIPGIASRTRTGGSATAITDLLRVARAGNQQAKELLAERARVLGGAVALLRDLLNPDEVVVGGQAFTEYPEAMEQVEAAFTAGSVLAPRDIRVTVFGNRVQEAGAGIVSLSGLYADPLGALRRSGALDARLQDTAPEALA
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEHCCCCCCCCCCCCCEEEEEHCCCCEEEEEEHCCCEEEEEEHCCCCCEEEEEEEHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHCCCCCHCCCCCEEHCCCCCCCCCCHHHHHHHHHCCCEEEHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEHCCCCEEEEEHCCEEHCCCCCCCCEEEEHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHCCCCCEEEHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCC
Conf.Score	998888887788888877778888877888999999999999999999699999999999999999999999999998997885677788999999768999689868999998698899999889998899999977999989999999999999999859887799998589855589988986889998888999999986998999654789999999988766788877599999688865899999978858999865555775799999988446665499999999999666776556655887789999999999899999999999999999999999998698879986667778899999999999996778888589856689544556899999999855555554567777667778886679
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MYSTNRTSQSLSRKPGRKHQLRSHRYVMPPSLHLSDSAAASVFRAVRLRGPVGRDVIAGSTSLSIATVNRQVIALLEAGLLRERADLAVSGAIGRPRVPVEVNHEPFVTLGIHIGARTTSIVATDLFGRTLDTVETPTPRNAAGAALTSLADSADRYLQRWRRRRALWVGVTLGGAVDSATGHVDHPRLGWRQAPVGPVLADALGLPVSVASHVDAMAGAELMLGMRRFAPSSSTSLYVYARETVGYALMIGGRVHCPASGPGTIAPLPVHSEMLGGTGQLESTVSDEAVLAAARRLRIIPGIASRTRTGGSATAITDLLRVARAGNQQAKELLAERARVLGGAVALLRDLLNPDEVVVGGQAFTEYPEAMEQVEAAFTAGSVLAPRDIRVTVFGNRVQEAGAGIVSLSGLYADPLGALRRSGALDARLQDTAPEALA
Prediction	651544445534553455442544243323353024202320031027532100020063274333101410453044300312353536444322010020133000000124422100000000414212424241457404400420050044006615753000000000010137402020010126414015203720500000011000000001000104426301000000001100000002000010310000002030320400120000000004000410352264453333244424221042004006621410130022003100101001000331411420220040043004302510452012451602105025200000000002330143003103412334451463526648
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEHCCCCCCCCCCCCCEEEEEHCCCCEEEEEEHCCCEEEEEEHCCCCCEEEEEEEHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHCCCCCHCCCCCEEHCCCCCCCCCCHHHHHHHHHCCCEEEHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEHCCCCEEEEEHCCEEHCCCCCCCCEEEEHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHCCCCCEEEHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCC
MYSTNRTSQSLSRKPGRKHQLRSHRYVMPPSLHLSDSAAASVFRAVRLRGPVGRDVIAGSTSLSIATVNRQVIALLEAGLLRERADLAVSGAIGRPRVPVEVNHEPFVTLGIHIGARTTSIVATDLFGRTLDTVETPTPRNAAGAALTSLADSADRYLQRWRRRRALWVGVTLGGAVDSATGHVDHPRLGWRQAPVGPVLADALGLPVSVASHVDAMAGAELMLGMRRFAPSSSTSLYVYARETVGYALMIGGRVHCPASGPGTIAPLPVHSEMLGGTGQLESTVSDEAVLAAARRLRIIPGIASRTRTGGSATAITDLLRVARAGNQQAKELLAERARVLGGAVALLRDLLNPDEVVVGGQAFTEYPEAMEQVEAAFTAGSVLAPRDIRVTVFGNRVQEAGAGIVSLSGLYADPLGALRRSGALDARLQDTAPEALA

1z05A

0.22

0.19

0.88

3.19

MUSTER

-------------------------------DHIKQINAGRVYKLIDQKGPISRIDLSKESELAPASITKITRELIDAHLIHETTVQEA-ISRGRPAVGLQTNNLGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHEIDQDDVLARLLFEIEEFFQTYALDRVTSIAITLPGLVNSEQGIVLQPHYNVKNLALGPEIYKATGLPVFVANDTRAWALAEKLFGHSQ---DVDNSVLISIHHGLGAGIVLDGRVLQGRHGIGELGHIQIDPCHCGNYGCLETVASSQAIRDQVTARIQAGEPSCL--ATVEEISIEDICAAAADGDPLAVDVIQQLGRYLGAAIAIVINLFNPEKILIGGVINQAKSILYPSIEQCIREQSLHQDLKLVESRFYKQATMPGAALIKQALYDGLLLMKVVEG----------------

1z05A

0.21

0.19

0.88

6.01

dPPAS

-------------------------------DHIKQINAGRVYKLIDQKGPISRIDLSKESELAPASITKITRELIDAHLIHETTVQEA-ISRGRPAVGLQTNNLGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHEIDQDDVLARLLFEIEEFFQTYALDRVTSIAITLPGLVNSEQGVLQMPHYNVKNLALGPEIYKATGLPVFVANDTRAWALAEKLFGHSQ---DVDNSVLISIHHGLGAGIVLDGRVLQGRHGIGELGHIQIDPCHCGNYGCLETVASSQAIRDQVTARIQAGEPSCL--ATVEEISIEDICAAAADGDPLAVDVIQQLGRYLGAAIAIVINLFNPEKILIGGVINQAKSILYPSIEQCIREQSLHQDLKLVESRFYKQATMPGAALIKQALYDGLLLMKVVEG----------------

1z05A

0.21

0.19

0.88

5.62

wdPPAS

-------------------------------DHIKQINAGRVYKLIDQKGPISRIDLSKESELAPASITKITRELIDAHLIHETTVQEA-ISRGRPAVGLQTNNLGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHEIDQDDVLARLLFEIEEFFQTYALDRVTSIAITLPGLVNSEQGVLQMPHYNVKNLALGPEIYKATGLPVFVANDTRAWALAEKLFGHSQ---DVDNSVLISIHHGLGAGIVLDGRVLQGRHGIGELGHIQIDPCHCGNYGCLETVASSQAIRDQVTARIQAGEPSCL--ATVEEISIEDICAAAADGDPLAVDVIQQLGRYLGAAIAIVINLFNPEKILIGGVINQAKSILYPSIEQCIREQSLPQDLKLVESRFYKQATMPGAALIKQALYDGLLLMKVVEG----------------

1z05A

0.22

0.19

0.88

3.78

wMUSTER

-------------------------------DHIKQINAGRVYKLIDQKGPISRIDLSKESELAPASITKITRELIDAHLIHETTVQEAISR-GRPAVGLQTNNLGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHEIDQDDVLARLLFEIEEFFQTYALDRVTSIAITLPGLVNSEQGIVLQPHYNVKNLALGPEIYKATGLPVFVANDTRAWALAEKLFGHSQ---DVDNSVLISIHHGLGAGIVLDGRVLQGRHGIGELGHIQIDPCHCGNYGCLETVASSQAIRDQVTARIQAGEPSCL--ATVEEISIEDICAAAADGDPLAVDVIQQLGRYLGAAIAIVINLFNPEKILIGGVINQAKSILYPSIEQCIREQSLHQDLKLVESRFYKQATMPGAALIKQALYDGLLLMKVVEG----------------

1z05A

0.21

0.19

0.88

4.47

wPPAS

-------------------------------DHIKQINAGRVYKLIDQKGPISRIDLSKESELAPASITKITRELIDAHLIHETTVQEA-ISRGRPAVGLQTNNLGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHEIDQDDVLARLLFEIEEFFQTYALDRVTSIAITLPGLVNSEQGVLQMPHYNVKNLALGPEIYKATGLPVFVANDTRAWALAEKLFGHSQ---DVDNSVLISIHHGLGAGIVLDGRVLQGRHGIGELGHIQIDPCHCGNYGCLETVASSQAIRDQVTARIQAGEPSCL--ATVEEISIEDICAAAADGDPLAVDVIQQLGRYLGAAIAIVINLFNPEKILIGGVINQAKSILYPSIEQCIREQSLPQDLKLVESRFYKQATMPGAALIKQALYDGLLLMKVVEG----------------

1z05A

0.21

0.19

0.88

6.30

dPPAS2

-------------------------------DHIKQINAGRVYKLIDQKGPISRIDLSKESELAPASITKITRELIDAHLIHETTVQEA-ISRGRPAVGLQTNNLGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHEIDQDDVLARLLFEIEEFFQTYALDRVTSIAITLPGLVNSEQGVLQMPHYNVKNLALGPEIYKATGLPVFVANDTRAWALAEKLFGHSQ---DVDNSVLISIHHGLGAGIVLDGRVLQGRHGIGELGHIQIDPCHCGNYGCLETVASSQAIRDQVTARIQAGEPSCL--ATVEEISIEDICAAAADGDPLAVDVIQQLGRYLGAAIAIVINLFNPEKILIGGVINQAKSILYPSIEQCIREQSLHQDLKLVESRFYKQATMPGAALIKQALYDGLLLMKVVEG----------------

1z05A

0.21

0.19

0.88

4.31

PPAS

-------------------------------DHIKQINAGRVYKLIDQKGPISRIDLSKESELAPASITKITRELIDAHLIHETTVQEA-ISRGRPAVGLQTNNLGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHEIDQDDVLARLLFEIEEFFQTYALDRVTSIAITLPGLVNSEQGVLQMPHYNVKNLALGPEIYKATGLPVFVANDTRAWALAEKLFGHSQ---DVDNSVLISIHHGLGAGIVLDGRVLQGRHGIGELGHIQIDPCHCGNYGCLETVASSQAIRDQVTARIQAGEPSCL--ATVEEISIEDICAAAADGDPLAVDVIQQLGRYLGAAIAIVINLFNPEKILIGGVINQAKSILYPSIEQCIREQSLPQDLKLVESRFYKQATMPGAALIKQALYDGLLLMKVVEG----------------

4ijaA

0.17

0.14

0.82

6.35

Env-PPAS

---------------------------------PMNDNEKRVLREIYNHHNISRTQISKNLEINKATISSILNKLKYKSLVNEVG-------GGRKPILLKVNHLYGYFISLDLTYSSVEVMYNYFDGNVIKHESYDLPDEKVSSILSIIKKHIDIQEKLDTYNGLLGVSVSIHGVVDNEQH-------VTYGISIAKKIKEITNVPVVVENEANLSALYERNFNHNL---SYNNLIALSIHKGIGAGLIINNQLYRGANGAGEIGKTLVDNVEIF--HKIEDIFSQEALLHNLSNQLN------------EKMTLSKLIQFYNEKNPVVVEEMEQFINKIAVLIHNLNTQFNPNAIYINCPLFNEMPEILEAIKNQFKQYSRN-EIQIKLTSNVKFATLLGGTLAIIQKVLQINDIYLDIKA---------------

4ijaA

0.17

0.13

0.81

2.97

MUSTER

----------------------------------MNDNEKRVLREIYNHHNISRTQISKNLEINKATISSILNKLKYKSLVNEVG--------GRKPILLKVNHLYGYFISLDLTYSSVEVMYNYFDGNVIKHESYDLPDEKVSSILSIIKKHIDIQEKLDTYNGLLGVSVSIHGVVDNEQH--------TYGISIAKKIKEITNVPVVVENEANLSALYERNFNHNL---SYNNLIALSIHKGIGAGLIINNQLYRGANGAGEIGKTLVSKVEIF--HKIEDIFSQEALLHNLSNQLN------------EKMTLSKLIQFYNEKNPVVVEEMEQFINKIAVLIHNLNTQFNPNAIYINCPLFNEMPEILEAIKNQFKQYS-RNEIQIKLTSNVKFATLLGGTLAIIQKVLQ--------INDIYLDIKA-------

4ijaA

0.17

0.14

0.82

5.70

dPPAS

---------------------------------PMNDNEKRVLREIYNHHNISRTQISKNLEINKATISSILNKLKYKSLVNEVG-------GGRKPILLKVNHLYGYFISLDLTYSSVEVMYNYFDGNVIKHESYDLPDEKVSSILSIIKKHIDIQEKLDTYNGLLGVSVSIHGVVDNEQHVT-------YGISIAKKIKEITNVPVVVENEANLSALYERNFNHNL---SYNNLIALSIHKGIGAGLIINNQLYRGANGAGEIGKTLVDNVEIF--HKIEDIFSQEALLHNLSNQLN------------EKMTLSKLIQFYNEKNPVVVEEMEQFINKIAVLIHNLNTQFNPNAIYINCPLFNEMPEILEAIKNQFKQYSRN-EIQIKLTSNVKFATLLGGTLAIIQKVLQINDIYLDIKA---------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=0.13

Estimated TM-score = 0.73±0.11

Estimated RMSD = 6.7±4.0Å

Download Model 2

C-score=-1.56

Download Model 3

C-score=-0.04

Download Model 4

C-score=-2.36

Download Model 5

C-score=-1.65

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1z05A	0.863	1.14	0.213	0.879
2	1z6rA	0.781	2.02	0.212	0.829
3	2hoeA	0.683	3.03	0.176	0.772
4	4ijaA	0.675	3.44	0.157	0.788
5	2qm1B	0.629	2.75	0.216	0.696
6	3vgkA	0.619	2.39	0.210	0.673
7	3mcpA	0.599	2.66	0.163	0.660
8	3imxA	0.595	4.22	0.098	0.728
9	3o08B	0.594	4.43	0.113	0.735
10	3r8eA	0.590	2.44	0.142	0.642

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.21	11	2q2rA	BGC	Rep, Mult	174,175,212,213,244,245,246,282
2	0.19	9	2yhwA	ZN	Rep, Mult	267,273,275,280
3	0.04	2	2yhwA	CA	Rep, Mult	212,215,247,262,263
4	0.03	2	2yhyA	ADP	Rep, Mult	115,116,117,118,120,242,243,287,317,365
5	0.03	2	2gupA	SUC	Rep, Mult	113,120,217,361,395,396,398
6	0.03	2	3vgmA	BGC	Rep, Mult	174,175,212,213,214,245
7	0.02	1	2gupA	SUC	Rep, Mult	198,202,205,206,207,208
8	0.02	1	3vglA	ANP	Rep, Mult	115,116,117,118,242,243,287,288,290,316,317,320,362,363,366
9	0.02	1	3eo3C	UNX	Rep, Mult	221,225,226,393,394
10	0.02	1	2gupA	SUC	Rep, Mult	197,201,207,208,209,210

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.283	1zc6B	0.469	4.28	0.124	0.585	2.7.1.59	NA
2	0.228	2aa4B	0.580	2.64	0.226	0.637	2.7.1.60	114,246,282,361
3	0.127	1xc3A	0.566	2.61	0.162	0.619	2.7.1.4	NA
4	0.113	3eo3A	0.574	2.58	0.163	0.630	5.1.3.14,2.7.1.60	NA
5	0.068	2q2rA	0.576	3.51	0.088	0.671	2.7.1.2	NA
6	0.067	2ch5A	0.508	4.08	0.085	0.623	2.7.1.59	NA
7	0.066	3f9mA	0.585	4.34	0.102	0.724	2.7.1.2	NA
8	0.066	2e2oA	0.476	4.26	0.131	0.589	2.7.1.1	NA
9	0.066	1x9jB	0.511	4.39	0.113	0.642	2.7.2.7	213
10	0.066	2ivpA	0.461	4.40	0.131	0.575	3.4.24.57	NA
11	0.060	3en9A	0.467	4.48	0.113	0.591	3.4.24.57	NA
12	0.060	2d4wA	0.488	4.09	0.111	0.591	2.7.1.30	360,392
13	0.060	2hoeA	0.683	3.03	0.176	0.772	2.7.1.59	NA
14	0.060	3enhA	0.463	4.40	0.119	0.580	3.4.24.57	62
15	0.060	1x3mA	0.510	4.49	0.083	0.646	2.7.2.15,2.7.2.-	NA
16	0.060	2iirJ	0.509	4.45	0.098	0.642	2.7.2.1	NA
17	0.060	3hz6A	0.490	4.03	0.088	0.589	2.7.1.17	244,267
18	0.060	3enoA	0.473	4.42	0.100	0.589	3.4.24.57	116,264
19	0.060	1v4tA	0.429	5.73	0.075	0.589	2.7.1.2,2.7.1.1	NA
20	0.060	2aa4A	0.578	2.68	0.222	0.637	2.7.1.60	NA
21	0.060	1bdgA	0.573	4.43	0.121	0.715	2.7.1.1	NA
22	0.060	3jvpD	0.504	4.52	0.117	0.626	2.7.1.16	263,343,346
23	0.060	2w41B	0.490	3.94	0.092	0.587	2.7.1.30	NA
24	0.060	3hm8A	0.580	4.50	0.102	0.724	2.7.1.1	NA
25	0.060	2vwbA	0.463	4.37	0.115	0.582	3.4.24.57	NA
26	0.060	1tuuA	0.472	5.18	0.118	0.639	2.7.2.1	NA
27	0.060	2ap1A	0.562	3.33	0.151	0.651	2.7.1.59	NA
28	0.060	1woqA	0.499	2.73	0.216	0.559	2.7.1.63	NA
29	0.060	1ig8A	0.590	4.47	0.114	0.733	2.7.1.1	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.29	0.798	1.98	0.21	0.84	1z6rA	GO:0003677 GO:0005737 GO:0005975 GO:0006351 GO:0006355 GO:0046872
1	0.28	0.675	3.44	0.16	0.79	4ijaA	GO:0003677 GO:0003700 GO:0006351 GO:0006355
2	0.26	0.611	2.38	0.14	0.66	3r8eA	GO:0004340 GO:0016301 GO:0016310 GO:0016740 GO:0051156
3	0.23	0.683	3.03	0.18	0.77	2hoeA
4	0.22	0.566	2.61	0.16	0.62	1xc3A	GO:0000166 GO:0000272 GO:0005524 GO:0005975 GO:0008865 GO:0016301 GO:0016310 GO:0016740 GO:0046835 GO:0046872
5	0.22	0.622	2.38	0.21	0.68	3vgkB	GO:0000166 GO:0004340 GO:0005737 GO:0006096 GO:0016301 GO:0016310 GO:0042802 GO:0046872 GO:0051156
6	0.20	0.863	1.14	0.21	0.88	1z05A	GO:0003700 GO:0006355 GO:0046872
7	0.19	0.579	2.90	0.21	0.65	3vovB	GO:0016301 GO:0016310 GO:0046872
8	0.19	0.610	2.78	0.16	0.67	3mcpA	GO:0016301 GO:0016310
9	0.18	0.637	2.71	0.21	0.70	2qm1B	GO:0004340 GO:0005737 GO:0006096 GO:0016301 GO:0016310 GO:0046872 GO:0051156
10	0.16	0.598	3.05	0.18	0.68	2yhwA	GO:0000166 GO:0003824 GO:0004553 GO:0005524 GO:0005737 GO:0005829 GO:0006045 GO:0006047 GO:0006054 GO:0007155 GO:0008152 GO:0008761 GO:0009384 GO:0016301 GO:0016310 GO:0016740 GO:0016787 GO:0046835 GO:0046872
11	0.15	0.584	2.77	0.13	0.65	4htlA	GO:0000166 GO:0005524 GO:0005975 GO:0016301 GO:0016310 GO:0016740 GO:0047700
12	0.15	0.551	3.66	0.15	0.66	4db3A	GO:0000166 GO:0005524 GO:0005975 GO:0006044 GO:0008270 GO:0009254 GO:0016301 GO:0016310 GO:0016740 GO:0045127 GO:0046835 GO:0046872
13	0.13	0.532	3.16	0.15	0.61	3htvA	GO:0000166 GO:0004340 GO:0005524 GO:0005975 GO:0008787 GO:0016301 GO:0016310 GO:0016740 GO:0019200 GO:0019316 GO:0046835 GO:0051156
14	0.11	0.499	2.73	0.22	0.56	1woqA	GO:0016301 GO:0016310
15	0.07	0.578	2.68	0.22	0.64	2aa4A	GO:0000166 GO:0005524 GO:0005975 GO:0006051 GO:0006974 GO:0008270 GO:0009384 GO:0016301 GO:0016310 GO:0016740 GO:0019262 GO:0046835 GO:0046872
16	0.07	0.574	2.58	0.16	0.63	3eo3A	GO:0000166 GO:0003824 GO:0004553 GO:0005524 GO:0005737 GO:0005829 GO:0006045 GO:0006047 GO:0006054 GO:0007155 GO:0008152 GO:0008761 GO:0009384 GO:0016301 GO:0016310 GO:0016740 GO:0016787 GO:0046835 GO:0046872
17	0.07	0.562	3.33	0.15	0.65	2ap1A	GO:0000166 GO:0005524 GO:0005975 GO:0006044 GO:0008270 GO:0009254 GO:0016301 GO:0016310 GO:0016740 GO:0045127 GO:0046835 GO:0046872
18	0.06	0.326	7.08	0.04	0.53	3gf7A	GO:0016874

Consensus prediction of GO terms

Molecular Function	GO:0001071	GO:0004396	GO:0003677	GO:0046872	GO:0035639	GO:0032559	GO:0032550
GO-Score	0.55	0.51	0.49	0.45	0.43	0.43	0.43
Biological Processes	GO:1901135	GO:0019637	GO:0006355	GO:0009057	GO:0044262	GO:0016052	GO:0005976	GO:0016310
GO-Score	0.51	0.51	0.49	0.43	0.43	0.43	0.43	0.42
Cellular Component	GO:0044424
GO-Score	0.59

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.