I-TASSER results

I-TASSER results for job id Rv0314c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (220 residues)
MIVVWEHLCMNPEDDPEARIRELERPLADVARASELGGSQSGGYTYPPGPPPPPYSYGGP
FGGPSPRSSSGNRAWWILAAVVVVGVLVLVGGIAAFSAQRLSQGNFVVLSPTPSVSRAVP
TPTAQPATTLPPAGASLSVSGVNVNRTIACNDSIVSVSGMSNTVVITGHCTSLTVSGMRN
SVTVDSVDTIEAAGFNNEVTYHSGSPKISNAGGSNSVQQG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MIVVWEHLCMNPEDDPEARIRELERPLADVARASELGGSQSGGYTYPPGPPPPPYSYGGPFGGPSPRSSSGNRAWWILAAVVVVGVLVLVGGIAAFSAQRLSQGNFVVLSPTPSVSRAVPTPTAQPATTLPPAGASLSVSGVNVNRTIACNDSIVSVSGMSNTVVITGHCTSLTVSGMRNSVTVDSVDTIEAAGFNNEVTYHSGSPKISNAGGSNSVQQG
Prediction	CCCEHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHCCEEEEEHCCCCEEEEEHCCCEEEEEEEEEEEEEEHCCCEEEEEEHCEEEEEHCCCEEEHCCCCCEEEHCCCCCEEHCC
Conf.Score	9555544468988897999999986788877545778888999999999999999999999999999998887534689999999999999887645777888877778999988888888888887766788875899976858999869987999976988999978879999847858999677589999858599987998778866888678679
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MIVVWEHLCMNPEDDPEARIRELERPLADVARASELGGSQSGGYTYPPGPPPPPYSYGGPFGGPSPRSSSGNRAWWILAAVVVVGVLVLVGGIAAFSAQRLSQGNFVVLSPTPSVSRAVPTPTAQPATTLPPAGASLSVSGVNVNRTIACNDSIVSVSGMSNTVVITGHCTSLTVSGMRNSVTVDSVDTIEAAGFNNEVTYHSGSPKISNAGGSNSVQQG
Prediction	4110043041538842552146134413541444443354564543444441342435433542444465432210020123121221110000113234264343444443444534434444444444455544130444444340417544020314413020303034030324613020430440203034020304545140433146243568
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCEHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHCCEEEEEHCCCCEEEEEHCCCEEEEEEEEEEEEEEHCCCEEEEEEHCEEEEEHCCCEEEHCCCCCEEEHCCCCCEEHCC
MIVVWEHLCMNPEDDPEARIRELERPLADVARASELGGSQSGGYTYPPGPPPPPYSYGGPFGGPSPRSSSGNRAWWILAAVVVVGVLVLVGGIAAFSAQRLSQGNFVVLSPTPSVSRAVPTPTAQPATTLPPAGASLSVSGVNVNRTIACNDSIVSVSGMSNTVVITGHCTSLTVSGMRNSVTVDSVDTIEAAGFNNEVTYHSGSPKISNAGGSNSVQQG

2ndjA

0.12

0.05

0.47

1.30

dPPAS2

---------METTNGTETWYESLHAVLKALNATL----------HSNLLCRPGPGLGPDNQTEERRASLPGRDDNSYMYILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI--------------------------------------------------------------------------------------------------

4bhrA

0.07

0.03

0.36

1.15

dPPAS2

-------------------------------------------------------------------------------TAAQAYVRNVATAVEAERDPTTGALPQLPQACDQFVANPPASVTQCNVTANNDGVNTVTAQLTYGSVSFDSSTGQFSFQL-------------------------------------------------------------

1aklA

0.13

0.99

0.79

MUSTER

QSWYLINSSYSANVNPANGNYGRQTLTHEIGHTLGLSHPGNAGEGDPTYADATYAEDTRAYSVMSYWEEQNTGQDFKGAYSSAPLLDDIAAIQKLYGANLTTRTGDTVYGFNSNTERDFYSATSSSSKLVAGGNDTLDFSGFSQNQKINLNEKLSDVGGLKGNVSIAAGVTVENAIGGSDLLIGNDVA--VLKGGNDILYGGLGADQLWGGAGADTFVYG

2ndjA

0.12

0.05

0.47

0.71

dPPAS

---------METTNGTETWYESLHAVLKALNATL----------HSNLLCRPGPGLGPDNQTEERRASLPGRDDNSYMYILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI--------------------------------------------------------------------------------------------------

3jx8A2

0.15

0.08

0.53

0.80

wdPPAS

-----------------------------------------------------------------------------------------------ISKPTKVNREINAGSGAGSGNIIAKDIQLDNLSCSLASSGEIEVIGTVDRASFNVAGS--EIKAFD----CQARKAECNIASSGESVYATQILDANIVGSG-EIHYK-GDPEISKSIGSGSINKV

5dfzB

0.12

0.11

0.97

0.90

wMUSTER

WIDKFHIIGLTEKD--IWKIVALRGYVIRTARVMAANPNLKNVFVQLEPTSYHMHSPNHGLVKPERKVVVSNSYEGDVESIEKFLSTFKILPPLRDYKEFGPIQEIVRSPNMGNLRGKLIATLMENEPNSI---TSSAVSPGETPYLITGDQGVIKIWNSSLTYDCSSTVTQITMIPNFDAFAVSSKDKVNHYQQESEVKFLNCE-NLKNFGKNRAFVNE

3jx8A

0.16

0.14

0.83

wPPAS

EITFVGSADFE-QSDKAPYLS-VT--LFDYLVTEVEGG-------------------------KSIKKGFNNNSYDLRPTVYKIKSNS------ELKELNTVGSGSFIISKPTKVNREINAGSGNVELRGPVKGYKLECAGSGQLDNLSCSSGEIEVIGTSGEIKCQARKAECNIASSGESVYATQILDANIVGSG-EIHYK-GDPEISKSGSGSNKVK-

3pqiA2

0.13

0.05

0.38

1.05

dPPAS2

--------------------------------------------------------------------------------------------------------------------------TATDSSEEVDSEKVIISNNKQTYASFDPNGNISVYNTQGMKIDMTPN-SIVLTDAGGGKLTLQG----------GTMTYKGGT--VNLNGL--TITDG

1mv3A

0.16

0.13

0.80

0.90

PPAS

--------DGSPAATPEIRVNH---EPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFED------------------TFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLP--------PGFMFKVQADYTAT-----DTDELQLKAGD-VVLVIPFQNEQDEG

2rfqA1

0.13

0.12

0.91

0.90

Env-PPAS

------------HDSHEQRLDALLPTLRERAQETEDLRRIPDDSKALQETGFFRLLQPEQWGGYQADPVLFYSAVRKIASAGSTGWVSSIIGVHNWHLALFS---QQAQEDVWGNDTDVRISSSYAPGAGQVVDGGYTVNGAWAWSSGCDHASWAVLGGPGRPVDFVSFLIPYRIDGTGNTVVVEDV-FVPT---HRVLSFKASNLTAPLERNTAPVYK-

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-3.24

Estimated TM-score = 0.35±0.12

Estimated RMSD = 13.2±4.1Å

Download Model 2

C-score=-4.41

Download Model 3

C-score=-5.00

Download Model 4

C-score=-5.00

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1aklA	0.723	4.04	0.116	0.977
2	1go7P	0.723	3.83	0.120	0.968
3	1srpA	0.719	3.98	0.144	0.968
4	1omjA	0.714	3.96	0.129	0.950
5	2vdcA	0.526	5.73	0.057	0.891
6	1llwA	0.516	5.69	0.084	0.877
7	5cskA3	0.489	5.51	0.050	0.823
8	5i6fA	0.488	5.46	0.020	0.823
9	3vstA	0.484	5.58	0.083	0.800
10	3ff6A	0.483	5.48	0.051	0.809

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.12	6	1ea0A	F3S	Rep, Mult	177,178,179,180,196,199,210
2	0.10	5	3wmn1	CRT	Rep, Mult	20,24
3	0.04	2	4rkuL	CLA	Rep, Mult	23,27
4	0.02	1	3tvuB	B37	Rep, Mult	137,149,156,157
5	0.02	1	1kj4B	III	Rep, Mult	206,208
6	0.02	1	4qjsU	MG	Rep, Mult	28,32
7	0.02	1	3iylA	MYR	Rep, Mult	159,175
8	0.02	1	1lt6P	GAA	Rep, Mult	193,194
9	0.02	1	1lm1A	F3S	Rep, Mult	148,149,150,152,155,165,166
10	0.02	1	3pgqB	GY3	Rep, Mult	175,176,192,193
11	0.02	1	1vq9B	MG	Rep, Mult	210,212
12	0.02	1	5klgA	6UC	Rep, Mult	27,30
13	0.02	1	1b5fB	UUU	Rep, Mult	162,183
14	0.02	1	2vn4A	MAN	Rep, Mult	140,141
15	0.02	1	5ioeB	ZN	Rep, Mult	25,29

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1uyrA	0.488	5.40	0.056	0.791	6.4.1.2,6.3.4.14	NA
2	0.060	1k9yA	0.378	6.33	0.040	0.709	3.1.3.7	NA
3	0.060	2a7sA	0.478	5.38	0.061	0.791	6.4.1.3	NA
4	0.060	1xnvA	0.483	5.28	0.051	0.786	6.4.1.3	204
5	0.060	1omjA	0.714	3.96	0.129	0.950	3.4.24.40	24
6	0.060	1bheA	0.464	5.45	0.053	0.754	3.2.1.15	NA
7	0.060	2vdcA	0.526	5.73	0.057	0.891	1.4.1.13	NA
8	0.060	1ea0A	0.445	6.02	0.062	0.800	1.4.1.13	NA
9	0.060	3jurD	0.467	5.41	0.051	0.746	3.2.1.15	NA
10	0.060	1wmrA	0.463	5.68	0.062	0.777	3.2.1.57	NA
11	0.060	1on3E	0.480	5.39	0.077	0.791	2.1.3.1	194
12	0.060	1dboA	0.450	5.71	0.052	0.754	4.2.2.19	NA
13	0.060	1af0A	0.723	3.98	0.143	0.977	3.4.24.40	NA
14	0.060	1ofdA	0.512	5.74	0.090	0.868	1.4.7.1	NA
15	0.060	1xnyA	0.482	5.34	0.071	0.795	6.4.1.3	177
16	0.060	3hdaP	0.636	3.37	0.126	0.809	3.4.24.-	24
17	0.060	1go7P	0.723	3.83	0.120	0.968	3.4.24.-	NA
18	0.060	1l8aA	0.440	5.69	0.025	0.741	1.2.4.1	NA
19	0.060	1llwA	0.516	5.69	0.084	0.877	1.4.7.1	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.12	0.723	3.83	0.12	0.97	1go7P	GO:0004222 GO:0005509 GO:0005576 GO:0005615 GO:0006508 GO:0008233 GO:0008237 GO:0008270 GO:0016787 GO:0031012 GO:0046872
1	0.12	0.723	4.04	0.12	0.98	1aklA	GO:0001869 GO:0004222 GO:0005509 GO:0005576 GO:0005615 GO:0006508 GO:0008233 GO:0008237 GO:0008270 GO:0009405 GO:0010765 GO:0016787 GO:0045959 GO:0046872
2	0.12	0.723	3.98	0.14	0.98	1af0A	GO:0004222 GO:0005509 GO:0005576 GO:0005615 GO:0006508 GO:0008233 GO:0008237 GO:0008270 GO:0009405 GO:0016787 GO:0031012 GO:0046872
3	0.12	0.714	3.96	0.13	0.95	1omjA	GO:0004222 GO:0005509 GO:0005615 GO:0006508 GO:0008237 GO:0008270 GO:0016787 GO:0046872
4	0.07	0.636	3.37	0.13	0.81	3hdaP	GO:0004222 GO:0005509 GO:0005576 GO:0005615 GO:0006508 GO:0008233 GO:0008237 GO:0008270 GO:0016787 GO:0031012 GO:0046872
5	0.06	0.401	5.20	0.05	0.63	2quaA	GO:0004806 GO:0016787 GO:0046872
6	0.06	0.404	5.35	0.05	0.65	3a6zC	GO:0005509 GO:0046872
7	0.06	0.352	5.63	0.08	0.61	2mzeA	GO:0004222 GO:0004252 GO:0005576 GO:0005578 GO:0005615 GO:0006508 GO:0007568 GO:0008201 GO:0008233 GO:0008237 GO:0008270 GO:0009986 GO:0016787 GO:0022617 GO:0030574 GO:0031012 GO:0031667 GO:0044849 GO:0046872 GO:0060135 GO:0070062 GO:0071260
8	0.06	0.297	5.71	0.06	0.52	3wcxA	GO:0004767 GO:0005576 GO:0008081 GO:0016787
9	0.06	0.359	6.32	0.06	0.68	5evlA	GO:0008800 GO:0016787 GO:0017001 GO:0030288 GO:0046677
10	0.06	0.310	6.08	0.05	0.57	2pz8A	GO:0000166 GO:0003952 GO:0005524 GO:0006734 GO:0008795 GO:0009435 GO:0016874 GO:0030436
11	0.06	0.337	6.29	0.08	0.61	1fblA	GO:0004175 GO:0004222 GO:0005509 GO:0005576 GO:0005578 GO:0006508 GO:0008233 GO:0008237 GO:0008270 GO:0016787 GO:0030574 GO:0031012 GO:0032461 GO:0046872
12	0.06	0.334	5.89	0.05	0.60	1slmA	GO:0004175 GO:0004222 GO:0004252 GO:0005509 GO:0005576 GO:0005578 GO:0005615 GO:0006508 GO:0008233 GO:0008237 GO:0008270 GO:0010727 GO:0016787 GO:0022617 GO:0030574 GO:0031012 GO:0032461 GO:0046872 GO:0071732 GO:1903209
13	0.06	0.339	6.18	0.07	0.62	1su3B	GO:0004175 GO:0004222 GO:0004252 GO:0005509 GO:0005576 GO:0005578 GO:0006508 GO:0008233 GO:0008237 GO:0008270 GO:0016032 GO:0016787 GO:0022617 GO:0030574 GO:0031012 GO:0032461 GO:0044267 GO:0046872 GO:0050900
14	0.06	0.325	6.30	0.04	0.60	4fu4A	GO:0001503 GO:0001554 GO:0001649 GO:0001666 GO:0001958 GO:0001968 GO:0003417 GO:0004222 GO:0004252 GO:0005509 GO:0005518 GO:0005576 GO:0005578 GO:0005615 GO:0005764 GO:0005794 GO:0006508 GO:0007507 GO:0007567 GO:0008233 GO:0008237 GO:0008270 GO:0009612 GO:0016787 GO:0022617 GO:0030282 GO:0030574 GO:0031012 GO:0035116 GO:0042493 GO:0043171 GO:0043627 GO:0044267 GO:0044849 GO:0046581 GO:0046872 GO:0048306 GO:0050750 GO:0051216 GO:0060349 GO:0071498 GO:1904244
15	0.06	0.275	6.34	0.03	0.54	5g04R	GO:0000922 GO:0005634 GO:0005654 GO:0005680 GO:0005737 GO:0005813 GO:0005815 GO:0005819 GO:0005829 GO:0005856 GO:0007049 GO:0007062 GO:0007067 GO:0007399 GO:0008022 GO:0008284 GO:0010997 GO:0016567 GO:0019899 GO:0030154 GO:0031145 GO:0031915 GO:0040020 GO:0042787 GO:0042826 GO:0043161 GO:0043234 GO:0048471 GO:0050773 GO:0051301 GO:0051436 GO:0051437 GO:0051439 GO:0090129 GO:0097027 GO:1904668
16	0.06	0.315	5.79	0.04	0.55	2rjqA	GO:0004222 GO:0005178 GO:0005576 GO:0005578 GO:0005615 GO:0005788 GO:0006508 GO:0008201 GO:0008233 GO:0008237 GO:0008270 GO:0016787 GO:0022617 GO:0031012 GO:0036066 GO:0042742 GO:0046872 GO:0050840
17	0.06	0.278	6.21	0.06	0.50	2aigP	GO:0004222 GO:0005576 GO:0006508 GO:0008233 GO:0008237 GO:0016787 GO:0046872
18	0.06	0.279	6.23	0.06	0.50	4j4mB	GO:0004222 GO:0006508 GO:0008237 GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0008270	GO:0004222	GO:0005509
GO-Score	0.45	0.45	0.45
Biological Processes	GO:0051704	GO:0006508
GO-Score	0.46	0.45
Cellular Component	GO:0005615
GO-Score	0.45

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.