I-TASSER results

I-TASSER results for job id Rv0311

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (409 residues)
MSQSRYAGLSRSELAVLLPELLLIGQLIDRSGMAWCIQAFGRQEMLQIAIEEWAGASPIY
TKRMQKALNFEGDDVPTIFKGLQLDIGAPPQFMDFRFTLHDRWHGEFHLDHCGALLDVEP
MGDDYVVGMCHTIEDPTFDATAIATNPRAQVRPIHRPPRKPADRHPHCAWTVIIDESYPE
AEGIPALDAVRETKAATWELDNVDASDDGLVDYSGPLVSDLDFGAFSHSALVRMADEVCL
QMHLLNLSFAIAVRKRAKADAQLAISVNTRQLIGVAGLGAERIHRAMALPGGIEGALGVL
ELHPLLNPAGYVLAETSPDRLVVHNSPAHADGAWISLCTPASVQPLQAIATAVDPHLKVR
ISGTDTDWTAELIEADAPASELPEVLVAKVSRGSVFQFEPRRSLPLTVK

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSQSRYAGLSRSELAVLLPELLLIGQLIDRSGMAWCIQAFGRQEMLQIAIEEWAGASPIYTKRMQKALNFEGDDVPTIFKGLQLDIGAPPQFMDFRFTLHDRWHGEFHLDHCGALLDVEPMGDDYVVGMCHTIEDPTFDATAIATNPRAQVRPIHRPPRKPADRHPHCAWTVIIDESYPEAEGIPALDAVRETKAATWELDNVDASDDGLVDYSGPLVSDLDFGAFSHSALVRMADEVCLQMHLLNLSFAIAVRKRAKADAQLAISVNTRQLIGVAGLGAERIHRAMALPGGIEGALGVLELHPLLNPAGYVLAETSPDRLVVHNSPAHADGAWISLCTPASVQPLQAIATAVDPHLKVRISGTDTDWTAELIEADAPASELPEVLVAKVSRGSVFQFEPRRSLPLTVK
Prediction	CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHEHCCCCCCCCEEEEEEEHCCCCCEEEHCCCCCCHCCCCCCHHHHHHHHCCCCCCCCCCEEEHCCCCCEEEHCCCCCCCCCCCCCEEEEEEEHCCCCCCCCCCCCHHHHHHCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEHCCEEEEEHCCCCCCCCCCHCCCCCHHHHHHHHHHHHCCEEEEEEHCCCCCEEEEEEHCCCCCCCCCCEEEEEHCCCCEEEEHCCCCCCCEHC
Conf.Score	9755788755999999999999986666655589999997868899999999987586899999999899898755666554344789986456789998589977885345664446678758887764678888876434555887557657899999999998567999976899988888758888775445666455789999876677764445665777789999999999999999999999999998588689999999887766777889999999869999999999999845768765589999699899985887778874334786555589999998688699998556886899999879887778867899985886589867877675659
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSQSRYAGLSRSELAVLLPELLLIGQLIDRSGMAWCIQAFGRQEMLQIAIEEWAGASPIYTKRMQKALNFEGDDVPTIFKGLQLDIGAPPQFMDFRFTLHDRWHGEFHLDHCGALLDVEPMGDDYVVGMCHTIEDPTFDATAIATNPRAQVRPIHRPPRKPADRHPHCAWTVIIDESYPEAEGIPALDAVRETKAATWELDNVDASDDGLVDYSGPLVSDLDFGAFSHSALVRMADEVCLQMHLLNLSFAIAVRKRAKADAQLAISVNTRQLIGVAGLGAERIHRAMALPGGIEGALGVLELHPLLNPAGYVLAETSPDRLVVHNSPAHADGAWISLCTPASVQPLQAIATAVDPHLKVRISGTDTDWTAELIEADAPASELPEVLVAKVSRGSVFQFEPRRSLPLTVK
Prediction	7555414503253002000200000100122100200230126102400041022113200410350061545403200300102120113104020303152102010200000040331155103000120530314020203244041322231254267441101000203463452641331430342311514033024655132202110033041330011000200430000100000001100243364445203301332000001000420340171265041002004001002023102031443202034130342100010023721300300030020303041344454010202434543641330000111412303146434120308
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHEHCCCCCCCCEEEEEEEHCCCCCEEEHCCCCCCHCCCCCCHHHHHHHHCCCCCCCCCCEEEHCCCCCEEEHCCCCCCCCCCCCCEEEEEEEHCCCCCCCCCCCCHHHHHHCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEHCCEEEEEHCCCCCCCCCCHCCCCCHHHHHHHHHHHHCCEEEEEEHCCCCCEEEEEEHCCCCCCCCCCEEEEEHCCCCEEEEHCCCCCCCEHC
MSQSRYAGLSRSELAVLLPELLLIGQLIDRSGMAWCIQAFGRQEMLQIAIEEWAGASPIYTKRMQKALNFEGDDVPTIFKGLQLDIGAPPQFMDFRFTLHDRWHGEFHLDHCGALLDVEPMGDDYVVGMCHTIEDPTFDATAIATNPRAQVRPIHRPPRKPADRHPHCAWTVIIDESYPEAEGIPALDAVRETKAATWELDNVDASDDGLVDYSGPLVSDLDFGAFSHSALVRMADEVCLQMHLLNLSFAIAVRKRAKADAQLAISVNTRQLIGVAGLGAERIHRAMALPGGIEGALGVLELHPLLNPAGYVLAETSPDRLVVHNSPAHADGAWISLCTPASVQPLQAIATAVDPHLKVRISGTDTDWTAELIEADAPASELPEVLVAKVSRGSVFQFEPRRSLPLTVK

2qa2A2

0.12

0.10

0.85

0.59

MUSTER

-----------SDASVIVPAGLMLAGELRLGGVDVMVTGESRLGFTARTMEVFDQRG------ILPAFGPVETSTQGHFGGRPVDFGVLE----------GAHYGVKAVPQS----TTESVLEEWALGRGAELLRGH-TVRALTDEGDHVVVEVEGP----GPRSLTTRYVVGCDGGR------STVRKAAGFDFPGTSAGDPARQVSAYRRGRVLLAGDLPAGGQGMNVSVQDSVNLGWKLAAVVSG-AGLLDTYHEPVGRRLLMNTQAQGMLFMQPLRDVLSELIRYDEVSRHLAGMVS-HPLLGRMPHQELVRAHGKTSTTE--LLHPARGVLLDIADD-AEVREAATGWSDRVDIVTASLHDA------PPQGPLSDAR-VAWISPGSRAGLTFPAR--------

5gaoE

0.12

0.10

0.79

0.66

dPPAS

--------------------------------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-----AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA----AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTKEAYKKLSQKFHGTKSNKK--------------------------------------------

3u07A

0.10

0.08

0.78

0.52

wdPPAS

-------------------------------------------TNVDLAEDAYIYGYSIDEAYKFFYHTAV-------------ENNYPLEFQDTLHL------GWLDVAA-EPVVSVPDDEGRYWILH---TDGHYTNAAFSTRGTKG-AAQDWQGE-VPASVRV--LVKLG----RIAVNDEDAKVALNYDQWNIRTLSE-GKNGPKPVQRTYPDPKKSTWLERVNFVLCD-NADKQWLDKYQSIGVE---PCKTDFTPEQLKLAKVGEKKGEHLVELAPKTDARTLLGPRDIFAEGTYLGQWGLPPIYRKSDFDSIYVRFKAPNV--SEFWVTIYGNDN------AKNDLNRHSRGDRTKADKDGYYTIYSANEKGRADDPNFL--VPEKPFYAIR---QPEKVVK

5kodA

0.15

0.12

0.85

0.57

wMUSTER

KNKQKLQLIEELTSNADQVQRQVLEEILTRNA-----VEYLRRHLNGRTRETFKNIMPVIT---YEDI----PEINRIANGDKSPILSSKPISEFLTSSGT---GERKL------M---PTIEEELDRRSL------LYSLLMPVMSQ----------FVPGLENGKGMYFLFIKSESKTPGGLPRPVLTSYYKSSHFKERPYDP----YTNYTSPN-ETILCSDSYQSMYSQMLCGLCQHQEVLASGFIRAIKFLEKHWIELVRDIRTGTLSSL--ITDPSVREAVALKPSPKLADFVEFECKKSSWQGIIT-RLWPNTKYVDV--------IVTGTMSQYIPTLDYYSNGL-PLVCTMYASSECYFGVNL----RPLCKPSEVSYTLIPSMAYFEFLPQELVDLVVK

2zc0A

0.18

0.13

0.72

0.47

wPPAS

MDTKYLAGRANWIKGSALADVMKKASELQKKKL---IS-LA--------RAVLGEIAKEVLEKEPKSVMYNGI-LAAFLKKYD-HLEVSP---E---NIV---IG---LDLLGRVL-IDP-GD--V------TENPSYIN-TLLA--GAKIEGVGQKVKIPTGQNP----MVTMSMER--------LEIASKYDLLIIE----TAYNF----YEGPLKADIVAGTLSKVGFGEILKKVLMQKQPIDFC---AISQYI---EYLKRYFEKYHL--------EGALLGYK----RDIMLKALENH--L-PAEFT----------------KPIAGMFFL--PEGADGISFANELMERE--VVVVESGKN-AIRLNFSRPSKEEIPI-GIKKL--AKLY----KEKF-----

2fr0A1

0.17

0.08

0.44

0.74

dPPAS2

------------------------------------------------------------------------------------------------------------------------------------------DE---VSALRYRIRPTGAGEPARLDG----TWLVAKYAGTADETSTAAREALESAGARVRELVVDARCGRSVGEVAGLLAVDEAEPEEAPLALASLADTLSLVQAMVSAELGCPLSAVATGPFERVRNAAHGALWGVGRVIALEPAVWGGLAGSVAELARHLAAV-VSGGAGEDQLALRADGVYGR-------------------------------------------------------------------------------------

2qa2A2

0.13

0.11

0.86

0.61

PPAS

-----------SDASVIVPAGLMLAGELRLGGVDVMVTGESRLGFTARTMEVFDQRG------ILPAFGPVETSTQGHFGGRPVDFGVLE----------GAHYGVKAVPQS----TTESVLEEWALGRGAELLRGH-TVRALTDEGDHVVVEVEGPDGP---RSLTTRYVVGCDGGRS------TVRKAAGFDFPGTSAGDPARQVSAYRRGRVLLAGD--SAHVHLPAGGQGMNVSVQDSVNLGWKLAAVVSGRAP-AGLLDTYHEERHPVGRRLLMNTQAQGMLFLSGDRDVLSELIRYDEVSHPLL--HQELVRAHGKTSTTELLHPARGVLLDIADD-AEVREAATGWSDRVDIVTA-SLHDAPPQGPLSDARA---GYVAWISPGSRAGLTFPAR--------

4abnA

0.15

0.14

0.92

0.78

Env-PPAS

-DEEEEAKHVLQKLQGLVDRLYCFRDSYFETH--SVEDAGRKQQDVQEEMEKTLQQEAQALMLKGKALNVTPDYEVLLSKAVKLE----PELVEAWN-----QLGEV-YWKKGDVTSAH---TCFSGALTHCKNKVSLQNLSMVLRQLQTDSGDEH-SRHVMDSVRQAKLAVQMDVLDGR-----SWYILGNAYLSLYFNTGQNPKQQALSAYAQAEKVDRKASSN-PDLHLNRATLHKYEYGEALEGFSQAAALDPAW---PEPQQREQQLLEFLSRLTSLLESKGKT-KPKKLQSMLGSLRPHLGP--YQSAQKMTLELKPLSQPGVNSGVVVFSLTTEEKVPFTFGL--VDPCYAVMVYNVVQSWG--VLIGDSVAIPEPNLRHHQIRKGKDYSFSSRVETPLLLV

3b2sA

0.14

0.13

0.92

0.57

MUSTER

SQYPTIVSTFEQGLKRFSEAPWVAGQVKGNTG-TSFIVPFEDVPRVVVKLRD-SAPTIEGMRKAGYPMAMFDENIIAPRKTLPIGPGTGDPKPVILLQLN-FIKGTVNGQHG--AMDM--VGQDAVIRLLSKANDPFTEEEMTAMNLD---RKTIVPYLENYTIGPEVDHQIV----KADVAGGDAVLTPVSASWAFFTFSPLKDAATKTLDASTKFVSTDDLSAFIWKSASRVRLERIDGSAPTEFCRAVDARPAMGVSNNYHNSTIGEIANESLGATASRLRSELDMRQRTRGLATYLHNNPDKSNVSLTADADPSTSVMLSSWGLWDDFGL--LGKPETVRRP--IFEPVESLMYFMPKKPDGEFCAALSLRDE--------DMDRLKADKEWTAQYVG-------

2qa2A2

0.10

0.09

0.88

0.63

dPPAS

-----------SDASVIVPAGLMLAGELRLGGVDVMVTGESRLGFTARTMEVFDQRG------ILPAFGPVETSTQGHFGGRPVDFGVLEGAHYGVKAVPQS--------------TTESVLEEWALGRGAELLRGH-TVRALTDEGDHVVVEVEGPDGP---RSLTTRYVVGCDGGRS------TVRKAAGFDFPGTSAGDPARQVSAYRRGRVLLAGDMNVSVQDSVNLGWKLAAVVSGRAPAGLLDTYHEERHPVGRRLLMNTQAQGMLFDEMQPLRDVLSELIRYDEVSRHLAGMVSVDGGDHPLLGMRQELVRAHGKTSTTELLHPARGVLLDIADDAEVREAATGWSDRVDIVTASLHDAP--------PQGPLSDARAVLVRPDGYVAWISPGSRAGLTEAL

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-3.91

Estimated TM-score = 0.29±0.09

Estimated RMSD = 16.8±2.9Å

Download Model 2

C-score=-4.39

Download Model 3

C-score=-4.52

Download Model 4

C-score=-5.00

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2qa1A	0.754	3.40	0.097	0.878
2	2qa2A	0.754	3.49	0.083	0.880
3	3fmwC	0.741	3.45	0.085	0.863
4	4k2xA	0.739	3.66	0.117	0.878
5	5kowA2	0.716	3.45	0.082	0.836
6	1pn0C	0.716	4.15	0.065	0.880
7	2dkiA	0.707	4.32	0.076	0.885
8	4cy6A	0.702	4.47	0.077	0.885
9	3ihgA	0.682	4.65	0.092	0.873
10	2r0gA	0.679	4.51	0.101	0.863

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.06	7	3ihgC	FAD	Rep, Mult	140,174,175,187,219,220,224
2	0.05	6	2dkhA	FAD	Rep, Mult	139,140,174,175,176
3	0.05	6	1bkwA	FAD	Rep, Mult	44,45,47,48,172,173,174,224,225
4	0.05	6	1cj2A	FAD	Rep, Mult	140,175,176,177,183,184,220
5	0.03	4	1cj4A	FAD	Rep, Mult	20,44,45,46,49,224,225,226
6	0.03	4	1fohB	FAD	Rep, Mult	138,139,174,175,224,225,227
7	0.02	2	4dr5G	MG	Rep, Mult	268,270
8	0.02	2	2ardA	FDA	Rep, Mult	20,21,22,23,24,53,54,230,327,329,330,333
9	0.02	3	1pbbA	DOB	Rep, Mult	142,144,151,153,155,166
10	0.02	2	4qjsU	MG	Rep, Mult	252,256
11	0.02	3	2qa10	III	Rep, Mult	27,40,42,44,45,48,49,52
12	0.02	2	1l5yA	BF2	Rep, Mult	117,118
13	0.02	2	3h1iE	SMA	Rep, Mult	130,131
14	0.01	1	3cp8B	FAD	Rep, Mult	140,141,219
15	0.01	1	4bk1A	P3A	Rep, Mult	85,95,261,265,268,269,272,286,287,295,296
16	0.01	1	2rdqA	FE	Rep, Mult	100,101,155
17	0.01	1	4jbeB	CA	Rep, Mult	237,241
18	0.01	1	4nkbA	MG	Rep, Mult	149,150
19	0.01	1	2xr1A	ZN	Rep, Mult	104,108,155,175
20	0.01	1	3atrA	DD9	Rep, Mult	48,49,108,223

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.066	1d7lA	0.545	3.76	0.100	0.658	1.14.13.2	NA
2	0.060	1e7pA	0.443	5.50	0.080	0.614	1.3.99.1	NA
3	0.060	2e5vA	0.394	4.88	0.059	0.518	1.4.3.16	NA
4	0.060	1dodA	0.545	3.76	0.100	0.658	1.14.13.2	220
5	0.060	3g5sA	0.399	5.29	0.083	0.543	2.1.1.74	25
6	0.060	1o5wC	0.346	6.48	0.047	0.540	1.4.3.4	25
7	0.060	1pn0C	0.716	4.15	0.065	0.880	1.14.13.7	NA
8	0.060	3cmmC	0.427	6.66	0.068	0.670	6.3.2.19	50,119,225
9	0.060	2a8xA	0.390	6.28	0.081	0.584	1.8.1.4	NA
10	0.060	2gmhA	0.482	4.82	0.059	0.633	1.5.5.1	39,49,230
11	0.060	1nekA	0.414	4.94	0.058	0.550	1.3.99.1,	NA
12	0.060	1vqwA	0.419	5.70	0.090	0.594	1.14.13.-	NA
13	0.060	2e5vB	0.393	4.83	0.055	0.516	1.4.3.16	329
14	0.060	1feaA	0.391	5.92	0.045	0.570	1.8.1.12	NA
15	0.060	2vdcF	0.389	7.15	0.036	0.660	1.4.1.13	93
16	0.060	1vrqB	0.404	5.12	0.060	0.545	1.5.3.1	357
17	0.060	1yq3A	0.398	6.59	0.057	0.633	1.3.5.1	NA
18	0.060	1llwA	0.392	7.03	0.038	0.660	1.4.7.1	NA
19	0.060	2wdqA	0.422	5.24	0.063	0.577	1.3.99.1	NA
20	0.060	1zovA	0.349	6.25	0.036	0.533	1.5.3.1	303

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.14	0.754	3.40	0.10	0.88	4icyA	GO:0000166 GO:0016491 GO:0055114 GO:0071949
1	0.14	0.754	3.49	0.08	0.88	2qa2A	GO:0000166 GO:0016491 GO:0055114 GO:0071949
2	0.14	0.747	3.42	0.09	0.87	4x4jA	GO:0016491 GO:0055114 GO:0071949
3	0.13	0.741	3.56	0.09	0.87	4k5rB	GO:0000166 GO:0016491 GO:0055114 GO:0071949
4	0.13	0.754	3.42	0.09	0.88	2qa1A	GO:0000166 GO:0016491 GO:0055114 GO:0071949
5	0.13	0.740	3.71	0.11	0.88	4k2xB	GO:0000166 GO:0016491 GO:0055114 GO:0071949
6	0.12	0.707	4.32	0.08	0.89	2dkiA	GO:0016491 GO:0018668 GO:0042803 GO:0055114 GO:0071949
7	0.12	0.716	4.20	0.07	0.88	1fohC	GO:0004497 GO:0005737 GO:0016491 GO:0018662 GO:0019336 GO:0019439 GO:0055114 GO:0071949
8	0.11	0.703	4.40	0.07	0.88	4z2rA	GO:0004497 GO:0016491 GO:0055114 GO:0071949
9	0.11	0.697	4.45	0.07	0.88	4cy8A	GO:0004497 GO:0016491 GO:0055114 GO:0071949
10	0.10	0.682	4.65	0.09	0.87	3ihgA	GO:0000166 GO:0016491 GO:0016709 GO:0017000 GO:0055114 GO:0071949 GO:1901771
11	0.10	0.679	4.51	0.10	0.86	2r0gA	GO:0000166 GO:0004497 GO:0008168 GO:0016491 GO:0032259 GO:0055114 GO:0071949
12	0.07	0.545	3.76	0.10	0.66	1d7lA	GO:0004497 GO:0016491 GO:0018659 GO:0019439 GO:0043639 GO:0043640 GO:0044550 GO:0050660 GO:0055114 GO:0071949
13	0.07	0.526	4.24	0.10	0.66	3nixA	GO:0000166 GO:0016491 GO:0055114 GO:0071949
14	0.07	0.525	4.49	0.06	0.67	5evyX	GO:0016491 GO:0055114 GO:0071949
15	0.07	0.533	4.07	0.08	0.66	5dbjC	GO:0016491 GO:0016787 GO:0047651 GO:0055114
16	0.07	0.519	4.17	0.08	0.65	4bjzA	GO:0000166 GO:0004497 GO:0016491 GO:0018658 GO:0055114 GO:0071949
17	0.07	0.512	4.47	0.09	0.66	3rp6A	GO:0004497 GO:0004846 GO:0006144 GO:0016491 GO:0016709 GO:0019628 GO:0055114 GO:0071949
18	0.07	0.528	4.57	0.06	0.67	5fn0A	GO:0004497 GO:0004502 GO:0006569 GO:0009435 GO:0016491 GO:0019363 GO:0019805 GO:0034354 GO:0043420 GO:0050660 GO:0055114 GO:0071949

Consensus prediction of GO terms

Molecular Function	GO:0071949	GO:0016491
GO-Score	0.53	0.53
Biological Processes	GO:0055114
GO-Score	0.53
Cellular Component
GO-Score

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.