I-TASSER results

I-TASSER results for job id Rv0225

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (384 residues)
MSALRSVLLLCWRDIGHPQGGGSEAYLQRIGAQLAASGIAVTLRTARYPGAPRHELVDGV
RISRAGGRYSVYLWALLAMAAARCGLGPLRRVRPDVVVDTQNGWPFVARLLYGRRSLVLV
HHCHREQWPVAGRMMGRLGWYVESMLSPRLHRRNQYVTVSLPSARDLIALGVDSERIAVV
RNGLDEAPSPTLSGPRAPTPRVVVLSRLVPHKQIEDALAAVAELQPRIPGLHLDIVGGGW
WRQRLVDHVHRLDIADAVTFHGHVDDVTKHHVLQSSWVHLLPSRKEGWGLAVIEAAQHGV
PTIGYRSSGGLADSIVDGVTGILVDDRAELVAWLEQLLSDSVLRDQLGAKAQARSGEFSW
RQSAEALRSVLEAVQASRFVSGVV

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSALRSVLLLCWRDIGHPQGGGSEAYLQRIGAQLAASGIAVTLRTARYPGAPRHELVDGVRISRAGGRYSVYLWALLAMAAARCGLGPLRRVRPDVVVDTQNGWPFVARLLYGRRSLVLVHHCHREQWPVAGRMMGRLGWYVESMLSPRLHRRNQYVTVSLPSARDLIALGVDSERIAVVRNGLDEAPSPTLSGPRAPTPRVVVLSRLVPHKQIEDALAAVAELQPRIPGLHLDIVGGGWWRQRLVDHVHRLDIADAVTFHGHVDDVTKHHVLQSSWVHLLPSRKEGWGLAVIEAAQHGVPTIGYRSSGGLADSIVDGVTGILVDDRAELVAWLEQLLSDSVLRDQLGAKAQARSGEFSWRQSAEALRSVLEAVQASRFVSGVV
Prediction	CCCCCEEEEEEHCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEHCCCCCCCCCCCCCCEEEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHCCCCHHHHHHHHHCCCCCCEEEHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEHCCHHHHHHHHHHCCCCCCEEEHCCCCCCCCCCCCCCCCCCCCEEEEHCCCCHHHCHHHHHHHHHHHHHHCCCCEEEEHCCCHHHHHHHHHHHHHCCCCCEEHCCCCCHHHHHHHHHHCCEEEHCCCCCCCCHHHHHHHHHCCCEEEHCCCCCCCCCHCCCCCEEHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
Conf.Score	999876999998589998888479999999999999899799996899998864445997999868988877755666788999999999859999998799589999999779998599865677665555544556678899999999987969988989999999989997778996899986556888888889878999868765659899999999999989996999987985679999999998998869985899989999999989899987888888789999999799799977999864443689875777999999999999985999999999999999999699999999999999999855666889
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSALRSVLLLCWRDIGHPQGGGSEAYLQRIGAQLAASGIAVTLRTARYPGAPRHELVDGVRISRAGGRYSVYLWALLAMAAARCGLGPLRRVRPDVVVDTQNGWPFVARLLYGRRSLVLVHHCHREQWPVAGRMMGRLGWYVESMLSPRLHRRNQYVTVSLPSARDLIALGVDSERIAVVRNGLDEAPSPTLSGPRAPTPRVVVLSRLVPHKQIEDALAAVAELQPRIPGLHLDIVGGGWWRQRLVDHVHRLDIADAVTFHGHVDDVTKHHVLQSSWVHLLPSRKEGWGLAVIEAAQHGVPTIGYRSSGGLADSIVDGVTGILVDDRAELVAWLEQLLSDSVLRDQLGAKAQARSGEFSWRQSAEALRSVLEAVQASRFVSGVV
Prediction	864201000000211120300100110130052316240300300233642334222550201101131212222111011023003103624000000000000000010243240000000101213322311220131012002300220100000044004102624045530100000013631434654647210000000015314141003003301751260300101406216402510561404432222220356202401100000002223302000000000030213012111001100333401000420410040034006146104401620251046241630041015004402753326337
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCEEEEEEHCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEHCCCCCCCCCCCCCCEEEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHCCCCHHHHHHHHHCCCCCCEEEHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEHCCHHHHHHHHHHCCCCCCEEEHCCCCCCCCCCCCCCCCCCCCEEEEHCCCCHHHCHHHHHHHHHHHHHHCCCCEEEEHCCCHHHHHHHHHHHHHCCCCCEEHCCCCCHHHHHHHHHHCCEEEHCCCCCCCCHHHHHHHHHCCCEEEHCCCCCCCCCHCCCCCEEHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
MSALRSVLLLCWRDIGHPQGGGSEAYLQRIGAQLAASGIAVTLRTARYPGAPRHELVDGVRISRAGGRYSVYLWALLAMAAARCGLGPLRRVRPDVVVDTQNGWPFVARLLYGRRSLVLVHHCHREQWPVAGRMMGRLGWYVESMLSPRLHRRNQYVTVSLPSARDLIALGVDSERIAVVRNGLDEAPSPTLSGPRAPTPRVVVLSRLVPHKQIEDALAAVAELQPRIPGLHLDIVGGGWWRQRLVDHVHRLDIADAVTFHGHVDDVTKHHVLQSSWVHLLPSRKEGWGLAVIEAAQHGVPTIGYRSSGGLADSIVDGVTGILVDDRAELVAWLEQLLSDSVLRDQLGAKAQARSGEFSWRQSAEALRSVLEAVQASRFVSGVV

5d00A

0.21

0.19

0.92

2.90

MUSTER

--RKLKIGITC-----YPSVGGSGIIATELGKQLAEKGHEIHFITSS-------TYHPNIHFHEVENQYAVFKYPPYDLTLASKIAEVAERENLDIIHAHYALPHAVCAYLAKKRNIGIVTTLHGTDITVLGYD-----PSLKDLIRFAIESSDRVTAVSSALAAETYDLIKPEKKIETIYNFIKNAAIKEKHGILPDEKVVIHVSNFRKVKRVQDVIRVFRNIAGK-TKAKLLLVGDGPEKSTACELIRKYGLEDQVLMLGNQD--RVEDLYSISDLKLLLSEKESFGLVLLEAMACGVPCIGTN-IGGIPEVIKNNVSGFLVDDVTAATARAMSILEDEQLSNRFTKAAIEMLEN-SSKKIVSQYEQIYADLAE--------

3c4qA

0.22

0.21

0.95

5.26

dPPAS

----MRVAMISMHQPGTGDSGGMNVYILSTATELAKQGIEVDIYTRATRPGEIVRVAENLRVINIAAGPYEGLSKEELPTQLAAFTGGMLSFTYDLIHSHYWLSGQVGWLLRDLWRIPLIHTAHTLAAVDT-----PESEARRICEQQLVDNADVLAVNTQEEMQDLMHHYADPDRISVVSPGADVELYSPGNGIPLHTKVVAFVGRLQPFKGPQVLIKAVAALFDRDPDRNLRVIICG--GPTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAAR-VGGLPIAVAEGETGLLVDSPHAWADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSSLYNDAIANE------

3c4qA

0.23

0.22

0.95

4.90

wdPPAS

----MRVAMISMHTSPLGDSGGMNVYILSTATELAKQGIEVDIYTRATRPGEIVRVAENLRVINIAAGPYEGLSPTQLAAFTGGMLSFTRREKYDLIHSHYWLSGQVGWLLRDLWRIPLIHTAHTLAAVDT-----PESEARRICEQQLVDNADVLAVNTQEEMQDLMHHYADPDRISVVSPGADVELYSPGNGIPLHTKVVAFVGRLQPFKGPQVLIKAVAALFDRDPDRNLRVIICGG--PTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAAR-VGGLPIAVAEGETGLLVDSPHAWADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSSLYNDAIANE------

3c4qA

0.23

0.22

0.95

3.45

wMUSTER

-----RVAMISMHTSGTGDSGGMNVYILSTATELAKQGIEVDIYTRATRPGEIVRVAENLRVINIAAGPYEGLSPTQLAAFTGGMLSFTRREKYDLIHSHYWLSGQVGWLLRDLWRIPLIHTAHTLAAVDT-----PESEARRICEQQLVDNADVLAVNTQEEMQDLMHHYADPDRISVVSPGADVELYSPGNGIPLHTKVVAFVGRLQPFKGPQVLIKAVAALFDRDPDRNLRVIICG--GPTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAAR-VGGLPIAVAEGETGLLVDSPHAWADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSSLYNDAIANE------

3c4qA

0.23

0.22

0.95

3.85

wPPAS

----MRVAMISMHTSGTGDSGGMNVYILSTATELAKQGIEVDIYTRATRPGEIVRVAENLRVINIAAGPYEGLSPTQLAAFTGGMLSFTRRETYDLIHSHYWLSGQVGWLLRDLWRIPLIHTAHTLAAVDT-----PESEARRICEQQLVDNADVLAVNTQEEMQDLMHHYADPDRISVVSPGADVELYSPGNGIPLHTKVVAFVGRLQPFKGPQVLIKAVAALFDRDPDRNLRVIICGG--PTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAAR-VGGLPIAVAEGETGLLVDSPHAWADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSSLYNDAIANE------

3c4qA

0.23

0.22

0.95

6.32

dPPAS2

----MRVAMISMHQPGTGDSGGMNVYILSTATELAKQGIEVDIYTRATRPGEIVRVAENLRVINIAAGPYEGLSPTQLAAFTGGMLSFTRREKYDLIHSHYWLSGQVGWLLRDLWRIPLIHTAHTLAAVDT-----PESEARRICEQQLVDNADVLAVNTQEEMQDLMHHYADPDRISVVSPGADVELYSPGNGIPLHTKVVAFVGRLQPFKGPQVLIKAVAALFDRDPDRNLRVIICG--GPTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAAR-VGGLPIAVAEGETGLLVDSPHAWADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSSLYNDAIANE------

5d00A

0.20

0.18

0.92

3.70

PPAS

--RKLKIGITC-----YPSVGGSGIIATELGKQLAEKGHEIHFITSSTYH-------PNIHFHEVENQYAVFKYPPYDLTLASKIAEVAERENLDIIHAHYALPHAVCAYLAKKRNIGIVTTLHGTDITVL-----GYDPSLKDLIRFAIESSDRVTAVSSALAAETYDLIKPEKKIETIYNFIKNTAAKEKHGILPDEKVVIHVSNFRKVKRVQDVIRVFRNIAGK-TKAKLLLVGDGPEKSTACELIRKYGLEDQVLMLGNQD--RVEDLYSISDLKLLLSEKESFGLVLLEAMACGVPCIGTN-IGGIPEVIKNNVSGFLVDDVTAATARAMSILEDEQLSNRFTKAAIEMLEN-SSKKIVSQYEQIYADLAE--------

5d00A

0.20

0.18

0.92

5.26

Env-PPAS

--RKLKIGITCY-----PSVGGSGIIATELGKQLAEKGHEIHFITSSTYH-------PNIHFHEVENQYAVFKYPPYDLTLASKIAEVAERENLDIIHAHYALPHAVCAYLAKKRNIGIVTTLHGTDITVL-----GYDPSLKDLIRFAIESSDRVTAVSSALAAETYDLIKPEKKIETIYNFIKNTAAIKEKGILPDEKVVIHVSNFRKVKRVQDVIRVFRNIAGK-TKAKLLLVGDGPEKSTACELIRKYGLEDQVLMLGNQDR--VEDLYSISDLKLLLSEKESFGLVLLEAMACGVPCIGTN-IGGIPEVIKNNVSGFLVDDVTAATARAMSILEDEQLSNRFTKAAIEMLENESSKKIVSQYEQIYADLAE--------

3c4qA

0.23

0.22

0.95

2.90

MUSTER

----MRVAMISMHTSGTGDSGGMNVYILSTATELAKQGIEVDIYTRATRPGEIVRVAENLRVINIAAGPYEGLSPTQLAAFTGGMLSFTRREKYDLIHSHYWLSGQVGWLLRDLWRIPLIHTAHTLAAVDT-----PESEARRICEQQLVDNADVLAVNTQEEMQDLMHHYADPDRISVVSPGADVELYSPGNGIPLHTKVVAFVGRLQPFKGPQVLIKAVAALFDRDPDRNLRVIICG--GPTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAAR-VGGLPIAVAEGETGLLVDSPHAWADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSSLYNDAIANE------

5d00A

0.20

0.18

0.92

5.22

dPPAS

--RKLKIGITC-----YPSVGGSGIIATELGKQLAEKGHEIHFITSST-------YHPNIHFHEVENQYAVFKYPPYDLTLASKIAEVAERENLDIIHAHYALPHAVCAYLAKKRNIGIVTTLHGTDITVL-----GYDPSLKDLIRFAIESSDRVTAVSSALAAETYDLIKPEKKIETIYNFIKNTAAKEKHGILPDEKVVIHVSNFRKVKRVQDVIRVFRNIAGK-TKAKLLLVGDGPEKSTACELIRKYGLEDQVLMLGNQDR--VEDLYSISDLKLLLSEKESFGLVLLEAMACGVPCIGTN-IGGIPEVIKNNVSGFLVDDVTAATARAMSILEDEQLSNRFTKAAIEMLENESSKKIVSQYEQIYADLAE--------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 3 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.11

Estimated TM-score = 0.87±0.07

Estimated RMSD = 4.4±2.9Å

Download Model 2

C-score=-0.63

Download Model 3

C-score=0.90

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3c4qA	0.909	1.58	0.227	0.951
2	3s27A	0.880	2.67	0.145	0.977
3	5d00A	0.823	2.28	0.183	0.891
4	2jjmL	0.821	2.53	0.219	0.901
5	2aw3A	0.820	3.28	0.141	0.958
6	1ygpA	0.819	3.38	0.117	0.958
7	2amvA	0.818	3.46	0.137	0.961
8	4l22A	0.818	3.41	0.126	0.958
9	4bqeA	0.817	3.37	0.111	0.956
10	5dxfA	0.816	3.22	0.115	0.951

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.60	79	3s28G	UDP	Rep, Mult	21,22,25,189,205,206,207,212,237,263,264,269,286,289,290,291,294
2	0.16	23	2qzsA	GLC	Rep, Mult	22,23,124,125,159,182,211,286,287,288,289,290
3	0.08	15	2iw1A	U2F	Rep, Mult	21,22,23,25,26,124,159,205,211,212,237,238,264,265,269,286,287,289,290,291,294
4	0.06	13	3s27B	FRU	Rep, Mult	13,19,20,21,22,23,24,124,207
5	0.02	6	2g9vA	PO4	Rep, Mult	20,22,207,211,212,294
6	0.01	3	3okaB	GDD	Rep, Mult	207,212,236,237,263,264,266,269,291,294
7	0.00	1	3l01A	MTT	Rep, Mult	130,137,139,140,141,142,143,144,147,148,151
8	0.00	1	3cejB	AVF	Rep, Mult	321,322,337,340
9	0.00	1	2bisA	GLC	Rep, Mult	132,134,135
10	0.00	1	2bisC	GLC	Rep, Mult	76,132,134,135
11	0.00	1	3c4qA	MG	Rep, Mult	273,274,276,300

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.320	2gejA	0.801	3.11	0.208	0.911	2.4.1.57	21,266,299
2	0.298	2r4uA	0.796	3.27	0.212	0.930	2.4.1.21	22,99,206,212,284,289,306,312
3	0.105	1o6cA	0.665	4.33	0.099	0.849	5.1.3.14	182,323
4	0.105	3dzcA	0.690	4.20	0.124	0.870	5.1.3.14	NA
5	0.104	3dzcB	0.687	4.32	0.117	0.872	5.1.3.14	287,306
6	0.060	1e1yA	0.820	3.52	0.142	0.966	2.4.1.1	207,212
7	0.060	1bgtA	0.619	4.39	0.079	0.786	2.4.1.27	NA
8	0.060	1rzuB	0.761	3.67	0.170	0.924	2.4.1.21	22,182,289,312
9	0.060	1f0kB	0.610	4.77	0.134	0.815	2.4.1.227	16,20,27,290
10	0.060	2vg8A	0.695	4.82	0.080	0.922	2.4.1.218	27,294
11	0.060	2b3xA	0.418	6.58	0.057	0.677	4.2.1.3	NA
12	0.060	2ipyA	0.436	6.70	0.038	0.711	4.2.1.3	122
13	0.060	2c1zA	0.688	4.58	0.116	0.898	2.4.1.115	209,289,294
14	0.060	2vsfA	0.495	5.70	0.091	0.724	3.6.4.12	214,243
15	0.060	3beoA	0.682	4.47	0.132	0.872	5.1.3.14	271
16	0.060	2vkzG	0.447	6.16	0.030	0.680	2.3.1.38,3.1.2.14	NA
17	0.060	2uv8G	0.449	6.14	0.033	0.680	2.3.1.86	NA
18	0.060	2is4B	0.438	6.14	0.074	0.659	3.6.1.-	NA
19	0.060	1fa9A	0.822	3.37	0.134	0.958	2.4.1.1	207,212
20	0.060	1y8zA	0.686	4.04	0.102	0.854	2.4.1.26	NA
21	0.060	5acnA	0.414	6.35	0.051	0.635	4.2.1.3	369
22	0.060	1ixyA	0.620	4.45	0.080	0.786	2.4.1.27	NA
23	0.060	2is6A	0.443	6.01	0.083	0.664	3.6.4.12	NA
24	0.060	2r60A	0.659	5.07	0.138	0.917	2.4.1.14	160,354

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.50	0.810	3.12	0.20	0.94	2bisA	GO:0004373
1	0.47	0.839	2.73	0.21	0.93	3mboE	GO:0000166 GO:0016740 GO:0016757 GO:0071793
2	0.39	0.785	3.17	0.21	0.92	3okaA	GO:0006629 GO:0008654 GO:0009247 GO:0016740 GO:0016757 GO:0033164 GO:0043750 GO:0046488 GO:0097502
3	0.38	0.794	3.64	0.16	0.93	4pqgA	GO:0005737 GO:0005886 GO:0016020 GO:0016740 GO:0016757
4	0.37	0.795	2.82	0.15	0.90	2iv7A	GO:0008919 GO:0009103 GO:0009244 GO:0016740 GO:0016757
5	0.36	0.739	3.31	0.21	0.88	2xa2A	GO:0005975 GO:0006006 GO:0016740 GO:0016757
6	0.35	0.742	2.93	0.15	0.84	4xywA	GO:0009103 GO:0009243 GO:0016740 GO:0016757
7	0.34	0.750	3.92	0.17	0.92	5e9tA	GO:0005737 GO:0005886 GO:0016020 GO:0016740 GO:0016757
8	0.33	0.668	3.94	0.17	0.82	4nc9C	GO:0004377 GO:0005886 GO:0006629 GO:0008654 GO:0009247 GO:0009405 GO:0016020 GO:0016021 GO:0016740 GO:0016757 GO:0043750 GO:0046488 GO:0097502
9	0.32	0.796	3.27	0.21	0.93	3guhA	GO:0004373 GO:0005829 GO:0005978 GO:0006974 GO:0009011 GO:0016740 GO:0016757 GO:0033201
10	0.30	0.881	2.68	0.16	0.98	3s27H	GO:0001666 GO:0005829 GO:0005985 GO:0006970 GO:0009409 GO:0009413 GO:0009414 GO:0009506 GO:0009744 GO:0009749 GO:0010555 GO:0016157 GO:0016740 GO:0016757 GO:0046686 GO:0072708
11	0.26	0.818	3.28	0.15	0.94	4wacA	GO:0016740 GO:0016757 GO:0047265
12	0.26	0.704	4.75	0.14	0.94	4hlnA	GO:0004373 GO:0009501 GO:0009507 GO:0016757 GO:0019252
13	0.23	0.563	4.36	0.14	0.68	3c48A	GO:0000287 GO:0008375 GO:0010125 GO:0016740 GO:0016757 GO:0046872
14	0.17	0.815	3.65	0.15	0.99	4rbnA	GO:0005985 GO:0016157 GO:0016740 GO:0016757
15	0.16	0.790	3.49	0.18	0.95	3vueA	GO:0004373 GO:0009501 GO:0009507 GO:0009536 GO:0016740 GO:0016757 GO:0019252 GO:0033840
16	0.14	0.777	3.28	0.13	0.90	3oy2A
17	0.12	0.719	3.96	0.18	0.88	4nc9A	GO:0004377 GO:0005886 GO:0006629 GO:0008654 GO:0009247 GO:0009405 GO:0016020 GO:0016021 GO:0016740 GO:0016757 GO:0043750 GO:0046488 GO:0097502
18	0.10	0.659	5.07	0.14	0.92	2r60A	GO:0005985 GO:0016157
19	0.09	0.652	4.46	0.14	0.84	4nesA	GO:0005737 GO:0008761 GO:0016853
20	0.07	0.475	3.62	0.13	0.56	4zj8A	GO:0004373 GO:0004871 GO:0004930 GO:0005886 GO:0005887 GO:0007165 GO:0007186 GO:0007218 GO:0007268 GO:0007631 GO:0008188 GO:0016020 GO:0016021 GO:0016499 GO:0017046 GO:0032870 GO:0042277 GO:0045187 GO:0051480 GO:1901652

Consensus prediction of GO terms

Molecular Function	GO:0004373	GO:0000166	GO:0043750	GO:0033164	GO:0008919
GO-Score	0.50	0.47	0.39	0.39	0.37
Biological Processes	GO:0071793	GO:0009247	GO:0097502	GO:0008654	GO:0046488	GO:0009244
GO-Score	0.47	0.39	0.39	0.39	0.39	0.37
Cellular Component	GO:0005886	GO:0005737
GO-Score	0.38	0.38

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.