I-TASSER results

I-TASSER results for job id Rv0193c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (615 residues)
MIQISRDMSSLGQTATTQALPDNSDGIQLTKFAADDILPLEYAPPIGPELVSQDQLPAAW
AYKRFRDLDDKESYRRKLLQELTDALAAQGSEAAEIATAALRDLIDQMAEQGAVVLADIV
ESDDFLELVKRYDELMAREGSRSFIHRFLDLRRSPGMLTDPAVNGALVHPLMIALISYAV
GGPIRMIDARGKDAEPLSVLAQDNMLHIDNTPFNDEYKILITWRRGTAQGPAGQNFTFLP
GTHKLARTCFVNEDGVPWSSENASIFTTPDSIRKVFDAQRQLGGQDHPTVIEVTDSERPL
SGVFAAGSLVHHRFRTASGSARSCIILVFHRVADNPGRMVSDVEDSSDVSLSELLTRGVP
DESYQQRFIATLCAAADEIAELLLKWKKTPQRPVSLPLQTKQIDGARFEEWISAATKAPE
VREIRNRELTIPYGEVLSAEEFFDLIWRLMRFDKHGPLDLILYHDNREEPRKWARNLIRE
MSADRLYERLLGWLADIQQPRPADCLRPLQIHALISEVLKTLPLDEDQDPPADWHFDLLG
MSHAEAARSVKHLLEDVAEALLRCEDMAAYLSTSLFAFWAVDAAYSLDGRRNLVVKDCAR
RLLRHYTMLSLTCFQ

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MIQISRDMSSLGQTATTQALPDNSDGIQLTKFAADDILPLEYAPPIGPELVSQDQLPAAWAYKRFRDLDDKESYRRKLLQELTDALAAQGSEAAEIATAALRDLIDQMAEQGAVVLADIVESDDFLELVKRYDELMAREGSRSFIHRFLDLRRSPGMLTDPAVNGALVHPLMIALISYAVGGPIRMIDARGKDAEPLSVLAQDNMLHIDNTPFNDEYKILITWRRGTAQGPAGQNFTFLPGTHKLARTCFVNEDGVPWSSENASIFTTPDSIRKVFDAQRQLGGQDHPTVIEVTDSERPLSGVFAAGSLVHHRFRTASGSARSCIILVFHRVADNPGRMVSDVEDSSDVSLSELLTRGVPDESYQQRFIATLCAAADEIAELLLKWKKTPQRPVSLPLQTKQIDGARFEEWISAATKAPEVREIRNRELTIPYGEVLSAEEFFDLIWRLMRFDKHGPLDLILYHDNREEPRKWARNLIREMSADRLYERLLGWLADIQQPRPADCLRPLQIHALISEVLKTLPLDEDQDPPADWHFDLLGMSHAEAARSVKHLLEDVAEALLRCEDMAAYLSTSLFAFWAVDAAYSLDGRRNLVVKDCARRLLRHYTMLSLTCFQ
Prediction	CCCCCCHHHHHHHHHHCCCCCCCCCCEEEHCCCCCCCEEEHCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHCCCCCCCCCCCCCCCCCEHCCCCCHHHEEEEEEEEHCCEHCCCCCCCEEHCCCCCCCCCCEEHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEHCCCCCCEEEEHCHHHHHHHHHHCCCCCCCCEEEEEEEHCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEHCCCCCEHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
Conf.Score	976665578887766544678888754543345567666545778774447855786667775689977889999999999999997778589999999999999999987976763545758999999999999998777568999999876655457865465466799999999998787455543466789876666787476578874443688999868856589888756668777788756767898887777665557757899999986567899998589975899975798755667767654789988655788876446994565578777898489999875788875789999999867999999999985889998655688877899999999999986885444435886657888889999999999999987567666544566755667899999876575789999998877765467777578489999999988665555556776543455547779999999999999999999887558899999999999998754478656788999999999999999999869
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MIQISRDMSSLGQTATTQALPDNSDGIQLTKFAADDILPLEYAPPIGPELVSQDQLPAAWAYKRFRDLDDKESYRRKLLQELTDALAAQGSEAAEIATAALRDLIDQMAEQGAVVLADIVESDDFLELVKRYDELMAREGSRSFIHRFLDLRRSPGMLTDPAVNGALVHPLMIALISYAVGGPIRMIDARGKDAEPLSVLAQDNMLHIDNTPFNDEYKILITWRRGTAQGPAGQNFTFLPGTHKLARTCFVNEDGVPWSSENASIFTTPDSIRKVFDAQRQLGGQDHPTVIEVTDSERPLSGVFAAGSLVHHRFRTASGSARSCIILVFHRVADNPGRMVSDVEDSSDVSLSELLTRGVPDESYQQRFIATLCAAADEIAELLLKWKKTPQRPVSLPLQTKQIDGARFEEWISAATKAPEVREIRNRELTIPYGEVLSAEEFFDLIWRLMRFDKHGPLDLILYHDNREEPRKWARNLIREMSADRLYERLLGWLADIQQPRPADCLRPLQIHALISEVLKTLPLDEDQDPPADWHFDLLGMSHAEAARSVKHLLEDVAEALLRCEDMAAYLSTSLFAFWAVDAAYSLDGRRNLVVKDCARRLLRHYTMLSLTCFQ
Prediction	723134314301421232423443310201403341001030113014411447314222313422546424401420053025205365243212211303401430454101000000203202400540451155431210000002004323103235031000000000000100100020242424424224142621201023440331020000044044422524200000132323220212552431334443122336104301413452534532212325335200000020000000211365342110000000000220130013346433330042014324444221100000133243035204513444444331525534046620430251024024234035451203554313143004100300111222102000134225303400440054155530132010002203524253034154035004401530525444543332414434234332131023002200400330550420000000000000000204444342024001200100000000017
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCHHHHHHHHHHCCCCCCCCCCEEEHCCCCCCCEEEHCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHCCCCCCCCCCCCCCCCCEHCCCCCHHHEEEEEEEEHCCEHCCCCCCCEEHCCCCCCCCCCEEHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEHCCCCCCEEEEHCHHHHHHHHHHCCCCCCCCEEEEEEEHCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEHCCCCCEHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
MIQISRDMSSLGQTATTQALPDNSDGIQLTKFAADDILPLEYAPPIGPELVSQDQLPAAWAYKRFRDLDDKESYRRKLLQELTDALAAQGSEAAEIATAALRDLIDQMAEQGAVVLADIVESDDFLELVKRYDELMAREGSRSFIHRFLDLRRSPGMLTDPAVNGALVHPLMIALISYAVGGPIRMIDARGKDAEPLSVLAQDNMLHIDNTPFNDEYKILITWRRGTAQGPAGQNFTFLPGTHKLARTCFVNEDGVPWSSENASIFTTPDSIRKVFDAQRQLGGQDHPTVIEVTDSERPLSGVFAAGSLVHHRFRTASGSARSCIILVFHRVADNPGRMVSDVEDSSDVSLSELLTRGVPDESYQQRFIATLCAAADEIAELLLKWKKTPQRPVSLPLQTKQIDGARFEEWISAATKAPEVREIRNRELTIPYGEVLSAEEFFDLIWRLMRFDKHGPLDLILYHDNREEPRKWARNLIREMSADRLYERLLGWLADIQQPRPADCLRPLQIHALISEVLKTLPLDEDQDPPADWHFDLLGMSHAEAARSVKHLLEDVAEALLRCEDMAAYLSTSLFAFWAVDAAYSLDGRRNLVVKDCARRLLRHYTMLSLTCFQ

3jd8A

0.10

0.98

0.88

MUSTER

FFVYADYHTHFLYCVRAPASLNDTSLLHLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSS--VACSLGVFSYIGLPLTLIVIEVIPF----LVLAVGVDNIFILVQAYQRDERLQGETLDQ--QLGRVLGE---VAPSSETVAFFLGALSVMPAVLFAGLAVFIDEDGTSVQASESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVSMPDDSYMVDYFKSISQYLHAGPPVYFVLEEGH-DYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAIDDYFDWVKPQCRVPEGKQRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVATYFMTYHTVLQTSADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWSLNAVSLVNLVMSCGISVEFCSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKF

3ripA

0.09

0.07

0.87

0.72

dPPAS

---MIHELLLALSYPGSIFTWNKRSGLQVSQDFP--------FLHPSETSVLNRLCRLGTDYIRFTEFIEQYTGHGGLHGIYLRAFC----TGLDSVLQPYRQALLDLEQE--FLGDPHLSISHVNYFLDQFQLLFPSV---MVVVEQIKSQKIHG------------CQILETVYKHSCGG---------------LPPVRSALEKILAVCHGVMYKQLSAWLHGLLLDQHEEFFIK----QGSLKQFSLRVEILP-------SYIPVRVAEKILFVGESVQMFENNVNLTSILKNQE-----DTFAAELHRLKQQPLFSLVDF-----------EQVVDRIRSTVAEHLWKLMVEESDLLGQLKIIKDFYLLGRGELFQAFIDTAQH-----MLKTPPTAVTEHDVNVAFQQSAHKVLLDDDNLLPLLHLTIEYHGASGWAAL---GLSYKVQWPLHILFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKHLKSNQTDARNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNLLAQSFILLKPVFHCLNEILDLCHSFCSLLLDERGAAQLSILVKGFSRQSSLLFKILS

3ripA

0.11

0.09

0.80

0.68

wdPPAS

MI---HELLLALSYPGSIFTWNKRSGLQVSQDFP--FLHPSET-----SVLNR--CRLGTDYIRTGHGGLHGIYLRAFCTGLDSVLQPLDLEQEFLGHLSISHVNYFLDQF----------QLLFPSVMVVVEQIKSQKIHGETVYKHSC-------------GLPPVRSALEKILAVCH------------------------------GVMYK---QLSAW-HGLLLDQHEEFFIK----QGSLKQFSLRVEILP-------SYIPVRVAEKILFVGESVQ-----MFENNVNLTSILKNQEDTFAAELHRLKQQPLFSLVDF-----------EQVVDRIRSTVAEHLWKLMVEESDLLGQLKIIKDFYLGRGELFQAFIDTAQ------HMLKTPPTAVTEHDVNVAFQQSAHKVLLDDDNLLPLTIEYHGASGWAAL------GLSYKVQWPLHILFTPAVLEKYNVVFKYLLSVRIKWRLRNHMAFLVDNLQYYLQVDVL-ESQFSQLLHQINSTRDFESIRL----AHDHFLSNLLAQSFILLKPVFHCLNEILDLCHSFCSLVSQN-----------LLDERGAAQLSILVKGFSRQSSLLFKILSS

5ifeC

0.12

0.10

0.89

0.85

wMUSTER

ISNLAKALATMISTMRPDIDNMDEYVRNTTALGIPSLLPF-----LKAVCKSKKSWQGIKIVQQIAILMGCLPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYG---------IESFDSVLKPLWKGIRQKGLAAFLKAIGYLIP----LMDAEYANYYTREVMLILIREFQSMKKIVLKVVKQCCGTDGVEAN----YIKTEPFFKHF-----WQH--RMALDRRNYRQLVDT----------------TVELANKVGAAEIISRIVDDLKDEAEQYRKMVMELGAADIDHKLEEQLIDGILYAFQEQTTE-DSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAK------VRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEE-YPEVLGSILGA--LKAIVN--VIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCI-----DLVGRIADRGAEYVSAREWMRICFELLELLKAHK-------KAIRRATVNTFGYIAKAIGPHDVLARVCTTVAIAIVAETCSPFTVLPALMNEYRELNVQNGVLKSLSFLFEYIEMGKDYIYAVTPLLEDALMLVHRQTASAVVQHMS

5flzB

0.12

0.10

0.80

0.67

wPPAS

IL---KYVSYTLL---SALFPFDHEQIQI-----PSKIPNFES-----GLLHL--FEAGLLYQS-FRMLNISPMKKALIIEISEELQNNNLVSSGTVV--LKSLYREIYEN----------IIRLRIYCRFTEHLEELSGDTIELNIFKS-------------GDLTIRKIATNLFNSMI------------------------------SLYYE---YLMNWTKGLLRATYGE-F-FI--AENYHIPIEFNQERVP-A------FIPKELAYKIFMIGKSY------IFLEKYCKEVQWTNEFSKKYHVLYQSNSYRGISTEIINDQYSEIVNHTNQILNQKFH-----YRDVVF-------ALKNILLM--GKSDFMDALIEKAN------DILATPSDSLPNYKLTRVLQEAVQLSSLRHLMNSPLDARVLDLGHGSVGWDVFT--LDYILYPPLSLVLNVNRPQKEMLKSNDIINKLSRSILRTQFQQFNSKMESYYLNCII-EENFKEMTRKLQRTLNIDELESV--NTFLTN----SHKLFAPYPTSLVLLLNSVYEFVKVYCNLNSNGLLGKFNTNLKEI-VSQYKNFKDRLYIFRADLKNEELFLLS

3ripA

0.09

0.07

0.84

0.78

dPPAS2

MI---HELLLALSYPGSIFTWNKRSGLQVSQDFP-------FLHPSETSVLN-RLCRLGTDYIRGLHGIYLRAFCTGLDSVLQPYRQALLDEQEFLGDPHISHVNYFLDQF----------QLLFPSVMVVVEQIKSQKIHGCQILETVYKHSCGG--------LPPVRSALEKILAVCH------------------------------GVMYK---QLSAWLHGLLLDQHEEFFIK----QGSLKQFSLRVEILP-------SYIPVRVAEKILFVGESVQMFENNVNLTSILKNQE-----DTFAAELHRLKQQPLFSLVDF-----------EQVVDRIRSTVAEHLWKLMVEESDLLGQLKIIKDFYLGRGELFQAFIDTAQ------HMLKTPPTAVTEHDVNVAFQQSAHKVLLDDDNLLPLTIEYHGASGWAAL------GLSYKVQWPLHILFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKHLKSNQTDARNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNLLAQSFILLKPVFHCLNEILDLCHSFCSLLLDERGAAQLSILVKGFSRQSSLLFKILS

5flzB

0.11

0.09

0.83

0.84

PPAS

IL---KYVSYTLL---SALFPFDHEQIQI-----PSKIPNFES-----GLLHL--FEAGLLYQS-FRMLNISPMKKALIIEISEELQNYTAFVNNLVSSGLKSLYREIYEN----------IIRLRIYCRFTEHLEELSGDTIELNIFKS-------------GDLTIRKIATNLFNSMI------------------------------SLYYE---YLMNWTKGLLRATYGEFFIA----ENYHIPIEFNQERVP-------AFIPKELAYKIFMIGKSY------IFLEKYCKEVQWTNEFSKKYHVLYQSNSYRGISTNFFEIINDQYSEIVNHTNQILNQKFH--YRDVVFA-MGKSDFMDALIEKANDILATPSDSLPNYKLTRVQLSSLRHLMNSPRNSSVINGLDARVLDLGHGSVGWDVFTLDNVNRPFGRKEYLRIFNFLWRFKKNNYFY---QKEMLKSNDIIRNKLSRI--SILRTQFQQFNSKMESYYLNCII-EENFKEMTRKLQRTLNIDELESV--NTFLTNILSHKLFATQPYPTSLVLLLNSVYEFVKVYCNLNSNGLLGKFNTNLKEI-VSQYKNFKDRLYIFRADLKNEELFLLS

5ebzA

0.10

0.91

1.02

Env-PPAS

SCRLELSTKNRERWCHE---------IQMKKLNHANVVKA-CDVPEELNILIHDVPLLAMEYCSGGD----------LRKLLNKPENCCGKSQILSLLSDIGSGIRYLHENKIIIVLQDVGGKIIHKIVDQGELCTEFVGTLQYLA--------PELFENKPY-TATVDYWSFGTMVFECGQPFTWHEIKKKDPKCIFACEEMSRFSSHCSLIVEPMELMLNWQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTGSQELLSETGISL----DPRKPASQCVLDGVRGCDSYMVYLFDKSKTVY----EGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRL-------FQGQRAAMLSLLRYNANLTK--MKNTLISASQQLKAK----LEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGYLEDQIMSLHAEIMELQKSPYGRGDLMESLEQRAIDLYKQLKHRPDHSYSDSTEM---VKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLIACTQ

5dlqB

0.11

0.10

0.97

0.86

MUSTER

RENLSAQMSSVFQRYLALSWNFLPPTESWREALLDSRV-MELFFTVHRKIREDSDMAQLASLHGFPDEGSQVDYLAHFIEGLLNTINGIEIEDS--EAVGISSIISNLITVFPRNVLTAIPFSSFVNCLTHLTCSFGRSAAMVYMEAYDKLLESWLTLVRKGFFTQHAVQVFNSYIQCHLAAPDGTRNLTANGVASREEEEISELQEDDRDQFSDQLASVIAAEHQLQRHQQDNKTGYLLADDTQGETPLIPPEIMEYSIKHSS-EVDINTTLQILGSPGE-KASSIPGYSRTDSVIRLLSAVLRVSEVESRAIRADLTHLLSKDIVWFTYLLVDESTAFGADTEGSQWKVISNLSVWSSEQDLANDTVQLLVTLVERRERANLVIQCASRSPPLNFLSFAHMDTETKQQYWTEVLQPLQQRRVINQE---NFQQMCQQEEVKQEITATLEALCQIDNVAILFNFLMDFLNNCIGLMEVYKNTPETVNLIIEVFVEVAH-KQICYLGESKAMHLYEACLTLLQVYSKNNLGRQ---RIDVTAEEEQYQDLLLIMELLTNLLSK--------SAADVVLYGVNLILPLMSQDLLKFPTLCNQYYKLITFICEIFPE

4whjA

0.09

0.07

0.84

0.72

dPPAS

IGDQSSGKSSVLEALSGVALPVTRCPLVLKLKKQPCEAWAGRISYRNTELELQ-------------DPGQVEKEIHKAQNVMAGNGRGISHEL-------ISLEITSPEVPDLTIIDLPGITPRDIGLQIKALIKKYIQRQQTIN----------LVVVPCNVDIATTEALSMAHEVDPEGDRTIGILTK-----------------PDLMDRGTEKSVM----------------NVVRNLTYPLKKGYMIVKCRGQQEITNRLSLAEATKKEITFFQTH---PYFRVLLEEGSATVPRLAERLTTELIMHIQKSL-------------------PLLEGQIRESHQKATEELRRCGADIPSQEADKMFFLIEKIKMFNQDIEKLVEGEEVVRENETRLYNKIREDFKNWVGILATNTQKV----------------KNIIHEEVEKYEKQAAAAELLGFVNYKTFEIIVHQYIQQLVEPALSMLQKAMEIIQQAFINVAKKHFGEFFNLNQTVQSTIEDIKVKHTAKLQFRMEQMVFSFTEIGIHLNAYFLETSKRLANQIPFIIQYFMLRENGDSLQKAMMQILQEKNRYSWLLQETATKRRILKERIYRLT

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 1 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-0.55

Estimated TM-score = 0.64±0.13

Estimated RMSD = 9.0±4.6Å

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3jd8A	0.925	2.72	0.100	0.985
2	4mt1A	0.611	5.75	0.051	0.792
3	2j8sA	0.604	5.68	0.065	0.782
4	3k07A	0.588	5.95	0.063	0.774
5	4k0eA	0.558	6.18	0.057	0.751
6	3aqpA	0.497	4.77	0.077	0.598
7	2cse1	0.330	7.93	0.046	0.543
8	5a22A	0.315	7.69	0.033	0.499
9	2x2hA	0.315	7.45	0.040	0.491
10	5kf6A	0.311	7.29	0.044	0.475

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.08	5	2ckjD	FES	Rep, Mult	181,182,184,185,186,187,189,210
2	0.07	4	2ckjA	FES	Rep, Mult	544,545,546,570,571,572,573
3	0.03	2	2rdqA	FE	Rep, Mult	207,209,311
4	0.03	2	3kxyJ	III	Rep, Mult	604,605
5	0.02	1	1xp3A	ZN	Rep, Mult	146,203,207
6	0.02	1	2j62A	GSZ	Rep, Mult	458,460
7	0.02	1	2ohjA	FE	Rep, Mult	206,207
8	0.02	1	2ckjD	PO4	Rep, Mult	190,196,198,201,257
9	0.02	1	4dk4A	CA	Rep, Mult	469,472
10	0.02	1	2zt9G	III	Rep, Mult	506,514
11	0.02	1	2ckjC	FES	Rep, Mult	582,583,584,596,597,598,599
12	0.02	1	4bntD	36E	Rep, Mult	511,515
13	0.02	1	1izlA	CLA	Rep, Mult	567,568
14	0.02	1	1fx9B	SO4	Rep, Mult	471,475
15	0.02	1	3slrA	MG	Rep, Mult	203,209,334
16	0.02	1	2j62B	GSZ	Rep, Mult	453,460
17	0.02	1	3gjoB	III	Rep, Mult	575,578,579
18	0.02	1	3m4oA	C7P	Rep, Mult	113,114,117
19	0.02	1	5sy1A	CLR	Rep, Mult	518,521
20	0.02	1	3fg4C	ACD	Rep, Mult	575,605,606,609

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2vz8A	0.274	8.47	0.032	0.471	2.3.1.85	NA
2	0.060	3cmmC	0.310	7.58	0.042	0.488	6.3.2.19	190,254
3	0.060	3gqbA	0.298	7.19	0.062	0.447	3.6.3.14	NA
4	0.060	3b8eC	0.308	7.78	0.046	0.488	3.6.3.9	179,255
5	0.060	1eulA	0.270	8.15	0.038	0.455	3.6.3.8	NA
6	0.060	1itwB	0.279	7.21	0.046	0.419	1.1.1.42	208,214
7	0.060	2e1qA	0.299	7.43	0.033	0.463	1.17.3.2,1.17.1.4	NA
8	0.060	3ixzA	0.293	6.25	0.041	0.406	3.6.3.10	NA
9	0.060	1n1hA	0.329	7.59	0.037	0.522	2.7.7.48	NA
10	0.060	3b8eA	0.307	7.64	0.021	0.478	3.6.3.9	NA
11	0.060	2hg4C	0.289	7.81	0.037	0.463	2.3.1.94	247
12	0.060	3i4lA	0.278	7.27	0.055	0.424	3.6.3.14	NA
13	0.060	1w36C	0.276	7.83	0.039	0.452	3.1.11.5	184,252
14	0.060	1fo4A	0.302	7.44	0.029	0.468	1.17.1.4	NA
15	0.060	2b0tA	0.286	7.12	0.024	0.429	1.1.1.42	180,575
16	0.060	2np0A	0.259	7.71	0.020	0.411	3.4.24.69	NA
17	0.060	1ygpA	0.280	8.00	0.068	0.463	2.4.1.1	NA
18	0.060	3b8cB	0.283	7.81	0.037	0.454	3.6.3.6	571
19	0.060	2gv9B	0.287	7.79	0.037	0.463	2.7.7.7	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.23	0.925	2.72	0.10	0.99	3jd8A	GO:0001618 GO:0004872 GO:0004888 GO:0005319 GO:0005576 GO:0005635 GO:0005764 GO:0005765 GO:0005768 GO:0005783 GO:0005794 GO:0005886 GO:0005887 GO:0006486 GO:0006629 GO:0006897 GO:0006914 GO:0007041 GO:0007165 GO:0007628 GO:0008202 GO:0008203 GO:0008206 GO:0015248 GO:0015485 GO:0016020 GO:0016021 GO:0016032 GO:0016242 GO:0030301 GO:0031579 GO:0031902 GO:0031982 GO:0033344 GO:0042493 GO:0042632 GO:0045121 GO:0046686 GO:0046718 GO:0048471 GO:0060548 GO:0070062 GO:0071383 GO:0071404 GO:0090150 GO:2000189
1	0.07	0.610	5.67	0.07	0.79	3d9bA	GO:0005215 GO:0005886 GO:0005887 GO:0006810 GO:0006855 GO:0015238 GO:0015307 GO:0016020 GO:0016021 GO:0042493 GO:0042802 GO:0046618
2	0.07	0.593	5.56	0.06	0.76	3nogA	GO:0005215 GO:0005886 GO:0005887 GO:0006810 GO:0006855 GO:0015238 GO:0015307 GO:0016020 GO:0016021 GO:0042493 GO:0042802 GO:0046618
3	0.07	0.602	5.89	0.07	0.80	2v50D	GO:0005215 GO:0005886 GO:0006810 GO:0006855 GO:0015238 GO:0016020 GO:0016021 GO:0046677
4	0.07	0.611	5.75	0.05	0.79	4mt1A	GO:0005215 GO:0006810 GO:0016020 GO:0016021
5	0.07	0.591	5.92	0.07	0.78	3w9iF	GO:0005215 GO:0005886 GO:0006810 GO:0006855 GO:0015238 GO:0016020 GO:0016021 GO:0046677
6	0.07	0.575	6.34	0.06	0.78	4dnrA	GO:0005215 GO:0005375 GO:0005507 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0006878 GO:0008324 GO:0010272 GO:0010273 GO:0015080 GO:0015673 GO:0015679 GO:0016020 GO:0016021 GO:0046688 GO:0060003 GO:1902601
7	0.07	0.558	6.18	0.06	0.75	4k0eA	GO:0005215 GO:0006810 GO:0006812 GO:0008324 GO:0016020 GO:0016021 GO:0098655
8	0.07	0.545	6.23	0.07	0.73	4k0eB	GO:0005215 GO:0006810 GO:0006812 GO:0008324 GO:0016020 GO:0016021 GO:0098655
9	0.07	0.546	6.14	0.07	0.73	4k0eC	GO:0005215 GO:0006810 GO:0006812 GO:0008324 GO:0016020 GO:0016021 GO:0098655
10	0.06	0.210	7.51	0.04	0.32	5en5C	GO:0005215 GO:0005886 GO:0005887 GO:0006810 GO:0006855 GO:0015238 GO:0015307 GO:0016020 GO:0016021 GO:0042493 GO:0042802 GO:0046618
11	0.06	0.209	4.95	0.07	0.26	4r86B	GO:0005215 GO:0006810 GO:0016020 GO:0016021
12	0.06	0.156	6.32	0.03	0.22	3qntA	GO:0005886 GO:0006629 GO:0006695 GO:0008144 GO:0008202 GO:0008203 GO:0015918 GO:0016020 GO:0016021 GO:0016324 GO:0017137 GO:0030299 GO:0030301 GO:0030659 GO:0031410 GO:0031489 GO:0031526 GO:0042157 GO:0042493
13	0.06	0.148	6.41	0.06	0.21	3f7jA	GO:0016491 GO:0043892 GO:0055114
14	0.06	0.158	6.58	0.04	0.23	4mkmA	GO:0016020 GO:0016021 GO:0046872
15	0.06	0.126	6.12	0.06	0.17	4l0jA	GO:0000746 GO:0003677 GO:0003678 GO:0004386 GO:0005524 GO:0016818 GO:0032508
16	0.06	0.140	6.60	0.05	0.20	4ni5B	GO:0016491 GO:0055114
17	0.06	0.113	5.61	0.06	0.15	4zbgA	GO:0008080 GO:0016740
18	0.06	0.125	6.84	0.06	0.19	3bwwA	GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0099600	GO:0005319	GO:0038023	GO:0032934	GO:0043178	GO:0022857	GO:0090484
GO-Score	0.46	0.46	0.46	0.46	0.46	0.37	0.37
Biological Processes	GO:0010941	GO:0010507	GO:0008344	GO:0045184	GO:0016241	GO:0016192	GO:0072657	GO:0032846	GO:0032787	GO:0055098	GO:0030301	GO:0009101	GO:2000188	GO:0071396	GO:0048545	GO:0016125	GO:0032870	GO:0071407	GO:0044700	GO:0030260	GO:0007154	GO:0043413	GO:0010038	GO:0006464	GO:0007034	GO:0071402	GO:0019058	GO:1902652	GO:0090659	GO:0015893	GO:0055085
GO-Score	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.37	0.37
Cellular Component	GO:0098852	GO:0031967	GO:0044428	GO:0005770	GO:0010008	GO:0000323	GO:0031988	GO:1903561	GO:0098857	GO:0005887
GO-Score	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.46	0.33

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.