I-TASSER results

I-TASSER results for job id Rv0167

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (265 residues)
MTTSTTLGGYVRDQLQTPLTLVGGFFRMCVLTGKALFRWPFQWREFILQCWFIMRVGFLP
TIMVSIPLTVLLIFTLNILLAQFGAADISGSGAAIGAVTQLGPLTTVLVVAGAGSTAICA
DLGARTIREEIDAMEVLGIDPIHRLVVPRVLASMLVATLLNGLVITVGLVGGFLFGVYLQ
NVSGGAYLATLTLITGLPEVVIATIKAATFGLIAGLVGCYRGLTVRGGSKGLGTAVNETV
VLCVIALFAVNVILTTIGVRFGTGR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MTTSTTLGGYVRDQLQTPLTLVGGFFRMCVLTGKALFRWPFQWREFILQCWFIMRVGFLPTIMVSIPLTVLLIFTLNILLAQFGAADISGSGAAIGAVTQLGPLTTVLVVAGAGSTAICADLGARTIREEIDAMEVLGIDPIHRLVVPRVLASMLVATLLNGLVITVGLVGGFLFGVYLQNVSGGAYLATLTLITGLPEVVIATIKAATFGLIAGLVGCYRGLTVRGGSKGLGTAVNETVVLCVIALFAVNVILTTIGVRFGTGR
Prediction	CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
Conf.Score	9858999999999999999999999999999999985698758999999999988889999999999999999999999999897678999999999999999999999998765699999997567779999999899964456789999999999999999999999999999999849999999999999746789999999999999999999976796767984667889889999999999999999999999857899
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MTTSTTLGGYVRDQLQTPLTLVGGFFRMCVLTGKALFRWPFQWREFILQCWFIMRVGFLPTIMVSIPLTVLLIFTLNILLAQFGAADISGSGAAIGAVTQLGPLTTVLVVAGAGSTAICADLGARTIREEIDAMEVLGIDPIHRLVVPRVLASMLVATLLNGLVITVGLVGGFLFGVYLQNVSGGAYLATLTLITGLPEVVIATIKAATFGLIAGLVGCYRGLTVRGGSKGLGTAVNETVVLCVIALFAVNVILTTIGVRFGTGR
Prediction	7433440042025203400420130021013002212333230230030023003311220333333312210210131034131331003000302020311330010000210020001002231441030043240411310100223012202331133033113301211111224031120131023114331133033113301300020001110414432530151012000200021333133213313313448
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MTTSTTLGGYVRDQLQTPLTLVGGFFRMCVLTGKALFRWPFQWREFILQCWFIMRVGFLPTIMVSIPLTVLLIFTLNILLAQFGAADISGSGAAIGAVTQLGPLTTVLVVAGAGSTAICADLGARTIREEIDAMEVLGIDPIHRLVVPRVLASMLVATLLNGLVITVGLVGGFLFGVYLQNVSGGAYLATLTLITGLPEVVIATIKAATFGLIAGLVGCYRGLTVRGGSKGLGTAVNETVVLCVIALFAVNVILTTIGVRFGTGR

4ke2A

0.07

0.05

0.66

1.23

dPPAS2

TAADAAAAAATIAASAASVAAATAADDAAASIATINA-----ASAAAKSIAAAAAMAAKDTAAAAASAAAAAVASAAKALETINVKAAYAAATTANTAAAAAAATATTAAAAAAAKATIDNAAAAKAAAVATAVSDAA---ATAATAAAVAAATLEAAAAKAAATAVSAAAAAAAAAIAFAAAP---------------------------------------------------------------------------------

4bwzA2

0.13

0.10

0.77

1.04

PPAS

-----------------------------------------RLKDILAV-----GKEAFLVAVLGVALPFLGGYLYGLEI----FETLPALFLGTALVTSVGITARVLQELGVLSRPYSRIILGAAVIDDVLGLIVL-----ACVNGVAETGQELSALASPVVLVAGTVVTVIAILGKVL---GGFLGALTQGVRSALTVGCGMAPRGEVGLIVAALGLKAGA---VNEEEYAIVLFMVVFTTLFAPFALKPLIAWTERERAAKE

5cwbA

0.12

0.08

0.69

1.10

dPPAS2

VAEKVERLKRSGTSEDEIAEEVAREISEVIRTLKESGSSYEVICECVARIVAEIVEALKRSGTSEDEIAEIVARVISEVIRTLKESGSSYEVICECVARIVAEIVEALKRSGTS-EDEIAEIVARVISEVIRTLKESGSSYVQRIVEEIVEALKRSGTSEDEINEIVRRVKSEVERTLKESGS----------------------------------------------------------------------------------

3wdoA2

0.08

0.06

0.71

1.09

dPPAS2

GMVKGSFSKVLAEPRLLKLNFGIMCLHMLLMSTFVA------LPGQLADAG-FPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRKKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAGY-----KGTAMGVYSTSQFLGVAIGGSLGGWIDGMF-----------------DGQGVFLAGAMLAAVWLAVAS-------------------------------------------------

4bwzA2

0.16

0.11

0.72

1.04

dPPAS2

-----------------------------------------RLKDILAV--------GKEAFLVAVLGVALPFLGGYLYGLEIGFETLPALFLGTALVTSVGITARVLQELGVLSRPYSRIILGAAVIDDVLGLIVLAC--VNGVAETGASPVVLVAGTVVTVIAILGKVLGGFLGALTQGVRSALTVGCGMAP------------RGEVGLIVAALGLKAGA------------VNEEEYAIVLFMVVFTTLFAPFALKPLIAW

4yxxA

0.11

0.08

0.73

1.01

dPPAS2

--VSLEQALKILKVAAELGTTVEEAVKRALKLKTKLGVSLEQALKILEVAAELVEEAVKRALKLKTKLGLEQALKILEVAAKLGTTVEEAVKRALKLKTKLGSLEQALKILKVAAELGTT--VEEAVKRALKLKTKLGVSLLKILEVAAELVEEAVKRALKLKTKLLEQALKILEVAAKLGTTVEEAVKRALKLKTK--------------------------------------------------------------------

5aymA

0.12

0.11

0.98

0.84

MUSTER

THPRIQVVKWGVWLQFFAILAGMVFFGMLDGLVRAGGRESWLLSVLFIALALSGVMASLGSQITDISVGNDLAPSLVEKLTHFNSIDLATEVGAPILAGALFPLAGLFLIGLWNLVSFVPEYFLLRNVIQRSGLKIKVLGSFSDPIFWLILSYALLWVLLAAYLKDEETEIGLFRGLGAVFGLISTVSFPYLVR-RLGLISSSRWHLGFQGVTLGIAVTA----FAMGSTASVYVFLGCILLSRVGLYGFSNGEFELRQRLIPEG

4ke2A

0.07

0.05

0.73

0.85

dPPAS

IDPAARAAAAAAASKAAVTAADAAAAAATIAASAASVAA--ATAADDAAASIATINAASAAAKSIAAAAAMAAKDTAAAAASAAAAAVASAAKALETINVKAAYAAATTANTAAAAAAATATTAAAAAAAKATIDNAAAAKAAAVATAVSDAAATAATAAAVAAATLEAAAAKAAATAVSAAAAAAAAAIAFAAAP---------------------------------------------------------------------

4bwzA2

0.16

0.11

0.71

wdPPAS

-----------------------------------------RLKDILA------GKEAFLVAVLGVALPFLGGYLYGLEI----FETLPALFLGTALVTSVGITARVLQELGVLSRPYSRIILGAAVIDDVLGLIVLAC--VNGVAET-ASPVVLVAGTVVTVIAILGKVLGGFLGALTQGVRSALTVGCGMAP------------RGEVGLIVAALGLKAGA------------VNEEEYAIVLFMVVFTTLFAPFALKPLIAW

4n7wA

0.13

0.97

0.93

wMUSTER

FPVWALLLSVAAYFRPTTFTGIGPYVGPLLMLIMFAMGVTLRLDDFKRVLSR--PAPVAAATFLHYLIMPLTAWILAMLFR--MPPDLSAGMVLVGSVA-ASNVMIYLAVALSVTISAVSTLVGVFATPLLTRLYVISVDVLQIVVIPITAGLVIHHTFTKTVKRIEPYLPAMSMVCILAII-SAVVAGSQSHIASGFVVIIAVILHNGIGLLSGYWGGK-GFDES--KIYFSPLAALPGALFSVWHNLSGSLLAGYWSGKPVKK

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-4.03

Estimated TM-score = 0.28±0.09

Estimated RMSD = 15.9±3.2Å

Download Model 2

C-score=-4.38

Download Model 3

C-score=-4.69

Download Model 4

C-score=-4.73

Download Model 5

C-score=-3.76

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4czbA	0.555	4.37	0.058	0.766
2	4bwzA	0.553	4.27	0.107	0.755
3	4cz8A	0.547	4.33	0.074	0.740
4	1zcdA	0.540	4.15	0.077	0.721
5	4n7wA	0.536	4.67	0.097	0.743
6	3zuxA	0.534	4.64	0.102	0.736
7	3dhiA	0.463	5.07	0.066	0.698
8	1mhzD	0.458	5.44	0.085	0.717
9	2j2fA	0.457	5.12	0.048	0.668
10	1t0rA	0.448	5.54	0.060	0.713

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.06	3	2jk5C	L2C	Rep, Mult	142,149
2	0.06	3	3perA	FE	Rep, Mult	99,129,132
3	0.04	2	1xvgA	BRJ	Rep, Mult	133,134,137,210,213,214
4	0.04	2	3zuxA	TCH	Rep, Mult	97,99,212,244
5	0.04	2	5ezmA	MPG	Rep, Mult	212,215
6	0.04	2	2h6bA	3C4	Rep, Mult	247,250
7	0.04	2	5l8gV	CA	Rep, Mult	106,122,125
8	0.04	2	3m0fB	GSH	Rep, Mult	102,125,129
9	0.04	2	1mhy1	III	Rep, Mult	101,102,105,106,108,109,112,113,126,129
10	0.02	1	3mmhB	MG	Rep, Mult	103,104
11	0.02	1	4xk8L	CLA	Rep, Mult	72,101
12	0.02	1	3zuyA	PTY	Rep, Mult	166,170,173,257,260,261,264
13	0.02	1	4il9A	UNL	Rep, Mult	213,217,220
14	0.02	1	3ddlB	PX4	Rep, Mult	75,84
15	0.02	1	1uuxA	IMD	Rep, Mult	174,175
16	0.02	1	1nquA	RDL	Rep, Mult	209,210

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1syyA	0.418	4.73	0.045	0.585	1.17.4.1	NA
2	0.060	1vncA	0.413	6.12	0.063	0.691	1.11.1.10	72,100
3	0.060	1mhyD	0.457	5.42	0.080	0.713	1.14.13.25	NA
4	0.060	1kq3A	0.420	5.25	0.090	0.645	1.1.1.6	NA
5	0.060	2vuxB	0.393	5.37	0.028	0.589	1.17.4.1	NA
6	0.060	2uw1B	0.456	5.26	0.067	0.672	1.14.99.6	NA
7	0.060	3c46B	0.394	6.19	0.050	0.694	2.7.7.6	133,136
8	0.060	1fziA	0.456	5.38	0.080	0.713	1.14.13.25	NA
9	0.060	1biqB	0.409	4.99	0.043	0.585	1.17.4.1	NA
10	0.060	2j2fA	0.457	5.12	0.048	0.668	1.14.19.2,1.14.99.6	NA
11	0.060	1rsrB	0.411	4.86	0.049	0.581	1.17.4.1	NA
12	0.060	1h0nA	0.411	5.16	0.053	0.600	1.17.4.1	NA
13	0.060	1ca1A	0.422	5.46	0.055	0.641	3.1.4.3	NA
14	0.060	1mc0A	0.253	6.10	0.034	0.423	3.1.4.17	108,255
15	0.060	1khoA	0.411	5.26	0.041	0.619	3.1.4.3	NA
16	0.060	1sojL	0.412	6.00	0.061	0.683	3.1.4.17	88,222
17	0.060	1ta9B	0.420	5.31	0.069	0.638	1.1.1.6	NA
18	0.060	2ouyA	0.440	5.68	0.043	0.698	3.1.4.17	NA
19	0.060	1olpA	0.436	5.22	0.050	0.649	3.1.4.3	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.07	0.547	4.22	0.09	0.74	4cz8A	GO:0006810 GO:0006812 GO:0015299 GO:0016020 GO:0016021 GO:0055085 GO:1902600
1	0.07	0.553	4.27	0.11	0.75	4bwzA	GO:0006810 GO:0006812 GO:0015299 GO:0016020 GO:0016021 GO:0046872 GO:0055085 GO:1902600
2	0.07	0.544	4.44	0.07	0.74	4atvA	GO:0005886 GO:0006810 GO:0006811 GO:0006814 GO:0006885 GO:0015081 GO:0015297 GO:0015491 GO:0015992 GO:0016020 GO:0016021 GO:0035725 GO:0055085 GO:0098655
3	0.07	0.536	4.67	0.10	0.74	4n7wA	GO:0016020 GO:0016021
4	0.07	0.463	5.07	0.07	0.70	3dhiA	GO:0004497 GO:0006725 GO:0016491 GO:0019439 GO:0042203 GO:0046872 GO:0055114
5	0.06	0.385	5.40	0.05	0.58	3gw3A	GO:0001889 GO:0004853 GO:0005654 GO:0005737 GO:0005829 GO:0006779 GO:0006782 GO:0006783 GO:0008198 GO:0010039 GO:0014070 GO:0014075 GO:0016829 GO:0016831 GO:0032355 GO:0042168 GO:0045471 GO:0046502 GO:0046689 GO:0051597 GO:0060992 GO:0071243
6	0.06	0.420	4.99	0.06	0.62	1mhyB	GO:0004497 GO:0006725 GO:0006730 GO:0015049 GO:0016491 GO:0016709 GO:0055114
7	0.06	0.564	4.25	0.06	0.77	4czbB	GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006814 GO:0015297 GO:0015299 GO:0016020 GO:0016021 GO:0042802 GO:0055085 GO:1902600
8	0.06	0.448	5.54	0.07	0.71	2incA	GO:0004497 GO:0006725 GO:0016491 GO:0046872 GO:0055114
9	0.06	0.456	5.38	0.08	0.71	1fziA	GO:0004497 GO:0006725 GO:0006730 GO:0015049 GO:0015050 GO:0015947 GO:0016491 GO:0046872 GO:0055114
10	0.06	0.457	5.42	0.08	0.71	1mhyD	GO:0004497 GO:0006725 GO:0006730 GO:0015049 GO:0016491 GO:0046872 GO:0055114
11	0.06	0.291	6.38	0.06	0.51	2y27B	GO:0000166 GO:0003824 GO:0005524 GO:0008152 GO:0010124 GO:0016874 GO:0046872 GO:0047475
12	0.06	0.301	6.74	0.07	0.58	3ug4C	GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0046373 GO:0046556
13	0.06	0.444	5.41	0.11	0.70	2innA	GO:0006725 GO:0016491 GO:0046872 GO:0055114
14	0.06	0.268	5.32	0.07	0.40	2fckA	GO:0008080 GO:0016740 GO:0016746
15	0.06	0.274	5.86	0.03	0.44	4rpfA	GO:0000166 GO:0004413 GO:0005524 GO:0005737 GO:0006566 GO:0008652 GO:0009088 GO:0016301 GO:0016310 GO:0016740
16	0.06	0.368	5.97	0.04	0.60	3eddA	GO:0003824 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0046872 GO:0047798
17	0.06	0.252	4.47	0.04	0.36	2lznA	GO:0000287 GO:0003677 GO:0003678 GO:0003896 GO:0003899 GO:0005524 GO:0006260 GO:0006269 GO:0008270 GO:0016740 GO:0016779 GO:0032508 GO:0046872 GO:1990077
18	0.06	0.534	4.64	0.10	0.74	3zuxA	GO:0016020 GO:0016021

Consensus prediction of GO terms

Molecular Function	GO:0015299	GO:0046872	GO:0015491	GO:0015081	GO:0004497
GO-Score	0.13	0.13	0.07	0.07	0.07
Biological Processes	GO:0044763	GO:0044765
GO-Score	0.37	0.37
Cellular Component	GO:0031224
GO-Score	0.38

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.