I-TASSER results

I-TASSER results for job id Rv0090

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (256 residues)
MAKNQNRIRNRWELITCGLGGHVTYAPDDAALAARLRASTGLGEVWRCLRCGDFALGGPQ
GRGAPEDAPLIMRGKALRQAIIIRALGVERLVRALVLALAAWAVWEFRGARGAIQATLDR
DLPVLRAAGFKVDQMTVIHALEKALAAKPSTLALITGMLAAYAVLQAVEGVGLWLLKRWG
EYFAVVATSIFLPLEVHDLAKGITTTRVVTFSINVAAVVYLLISKRLFGVRGGRKAYDVE
RRGEQLLDLERAAMLT

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MAKNQNRIRNRWELITCGLGGHVTYAPDDAALAARLRASTGLGEVWRCLRCGDFALGGPQGRGAPEDAPLIMRGKALRQAIIIRALGVERLVRALVLALAAWAVWEFRGARGAIQATLDRDLPVLRAAGFKVDQMTVIHALEKALAAKPSTLALITGMLAAYAVLQAVEGVGLWLLKRWGEYFAVVATSIFLPLEVHDLAKGITTTRVVTFSINVAAVVYLLISKRLFGVRGGRKAYDVERRGEQLLDLERAAMLT
Prediction	CCCCCCCCCCCEHCEEEHCCCCEEHCCCCHHHHHHHCCCCCCCCCEEEEHCCCCEHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
Conf.Score	9875445555434445444565565898889988753346788646676558756578889998766898877667778999999999999999999999999999858769999999999999998789988885899999998599768999999999999999999999998666889999999999875899999985768899999999999999999998755777567889998789999999986789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MAKNQNRIRNRWELITCGLGGHVTYAPDDAALAARLRASTGLGEVWRCLRCGDFALGGPQGRGAPEDAPLIMRGKALRQAIIIRALGVERLVRALVLALAAWAVWEFRGARGAIQATLDRDLPVLRAAGFKVDQMTVIHALEKALAAKPSTLALITGMLAAYAVLQAVEGVGLWLLKRWGEYFAVVATSIFLPLEVHDLAKGITTTRVVTFSINVAAVVYLLISKRLFGVRGGRKAYDVERRGEQLLDLERAAMLT
Prediction	6555564241302121003322232316465036404153443420200203311314157524474224235344122200010000131021100111110001014444313411443141033030325432023004303514442021002102211301310010103533002000011111111110110034241310011231321121002122013143354125244456311403534537
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCEHCEEEHCCCCEEHCCCCHHHHHHHCCCCCCCCCEEEEHCCCCEHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
MAKNQNRIRNRWELITCGLGGHVTYAPDDAALAARLRASTGLGEVWRCLRCGDFALGGPQGRGAPEDAPLIMRGKALRQAIIIRALGVERLVRALVLALAAWAVWEFRGARGAIQATLDRDLPVLRAAGFKVDQMTVIHALEKALAAKPSTLALITGMLAAYAVLQAVEGVGLWLLKRWGEYFAVVATSIFLPLEVHDLAKGITTTRVVTFSINVAAVVYLLISKRLFGVRGGRKAYDVERRGEQLLDLERAAMLT

4wgvA

0.13

0.12

0.95

0.71

MUSTER

FVFEVFISSPQLTDILNGFVPHKEIVTNQGILYIALGI-IGATIMPHNLYLHSSIVQS-RKYDRHDNE---EKAQAIKYATIDSNLQLSIAFVVNCLLLTLGAALFFGTKTNDLGGFYDLYHALKTEPVLGATLGGVMSTLFAVAL---LASGQNSTITGTLAGQIVMEGFLNWLRRLITRSLAVIPVIICLIIFSEKIEQLLVFSQVFLSIALPFSLIPLQLATSNKKLMGP----FINKTWVNIISWTLIVILS

4ke2A

0.13

0.09

0.73

0.71

dPPAS

----------------------------------------------------------------------MNIDPAARAAAAAAASKAAVTAADAAAAAATIAASAASVAAATAADDAAASIATINAASAAAKSIAAAAAMAAKDTAAAAASAAAAAVASAAKALETINVKAAYAAATTANTAAAAAAATATTAAAAAAAKATIDNAAAAKAAAVATAVSDAAATAATAAAVAAATLEAAAAKAAATAVSAAAAAA

1t33A

0.12

0.09

0.79

0.67

wdPPAS

MNIPTTTTKGE-QLIAAALYG---LHATTRDIAALAGQNIAA-----------F------GS------------EDLYLACAQWIADFLGEKFRPHAEKAERLFSQPAPDRDAIRELILLACKNMIMLLTQEDTVNLSKFISREQLSPTSAYQLVHEQVIDPLHTHLTRLVAAYTGC----------------------ANDTRMILHTHALLGEVLAFRLGKETILLRTGWPQFDEEKAELIYQTVTCHIDLILH

4ui9O

0.14

0.13

0.98

0.72

wMUSTER

LSQKQAEFFLSQQASLLKNDETKALTPASKELNNLLKFNPDFAEA--NLRVQDFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQ--KRSDSYVLLEHSVKKAVHFGLLGIQSLVQQRAFAGANKLMDALKDSDLLHWKH-SELIDISIAQKTAIWRLYSTMALQQAQMLLSTESFAVALCHLAELHAEQGCAAASEVLKHLKERSQHAQLWMLC

3e7qB

0.15

0.11

0.77

0.69

wPPAS

MNQEVRFSRLE---------------P--EQRKALL-IEA----TLACL---------------KR------HG--FQG---VRKICAEAVSVGLINHH-KDALVAEA--LAVTGRVMRLLRGAIDTAPGGARPR-LSAFFEASFSAELLDPQLLDAWLAFWGAVGSIEAIGRVHDHSYGEYRALLVGVLRQLAEEGGW-ADFDAELAAISLSALLDGLWLESGLN----PATFTPRQGVQICEAWVDGLEAG---

4ke2A

0.12

0.09

0.73

0.97

dPPAS2

-----------------------------------------------------------MNIDPAARAAA----AAAASKAAVTAADAAAAAATIAASAASVAAATAADDAAASIATINAASAAAKSI-------AAAAAMAAKDTAAAAASAAAAAVASAAKALETINVKAAYAAATTANTAAAAAAATATTAAAAAAAKATIDNAAAAKAAAVATAVSDAAATAATAAAVAAATLEAAAAKAAATAVSAAAAAA

3e7qB

0.16

0.12

0.77

PPAS

MNQEVRFSRLE---------------P--EQRKALL--------TLACLK----------------------HG---FQGASVRKICAEAVSVGLINHH-KDALVAEA--LAVTGRVMRLLRGAIDTAPGGARPR-LSAFFEASFSAELLDPQLLDAWLAFWGAVGSIEAIGRVHDHSYGEYRALLVGVLRQLAEEGG----WADFDAELAAISLSALLDGLWLESGLN-PATFTPRQGVQICEAWVDGLEAGAHR

2hn1A

0.13

0.11

0.89

0.60

Env-PPAS

TGEKKVE-ETKITAAIYDEKSVEFKELEVGELESVVRSALALNKLWIDVVG-------------VHDESLI---AKICEFLGIHPLAAEDILNTAHLFLVLKILLYNETLEIDQLSLVLKKNLVAT---FEEREYWILDSIRSRLKSGG-RMRKLAGDYLAYTILDAVVDSYFEALLKISDEIEVLEDEVVSGGKIHSLKRE--ILAFRNAVWPLRDVLSFFTRVEHELIGEEVKVYYRDVYDHAVRLME------

5an8A

0.11

0.99

0.69

MUSTER

VQLEGIPNLEGL-LKLAAKEGKIEIFSAPCQSLSRKFTEWCYGPVRVSLYDLASVDSWEENSSRSPHRHRMVVLEPLNKLLQAKWDRLIPRFCFNFLCYLVYMLIFTAVAYHQPALAGNSMLLLGHILILLGGVYLLLGQLWYFWRRRLFMDSYFEILFLLQALLTVLSQVLCFLAIEW-YLPLLVSSLVLGWLNLLYYTRGFQHTGIYSVMIQKVILRDLLRFLLVYLLFGFAVALVSLSREPPYRSILDASLEL

5cwmA

0.13

0.11

0.87

0.67

dPPAS

-------------------------DPEDELKRVEKLVKEAEELL---------RQAKEKGSEEDLEKALRTAEEAAREAKKVLEQAEKEGDPEVALRAVELVVRVAELLLRIAKESGSEEALERALRVAEEAARLAKRVLELAEKQGDPEVALRAVELVVRVAELLLRIAKESGSEEALERALRVAEEAARLAKRVLELAEGDPEVARRAVELVKRVAELLERIARESGSEEAKERAERVREEARELQERVKELR

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-4.30

Estimated TM-score = 0.26±0.08

Estimated RMSD = 16.5±2.9Å

Download Model 2

C-score=-4.86

Download Model 3

C-score=-4.97

Download Model 4

C-score=-3.63

Download Model 5

C-score=-4.09

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4kf7A	0.625	4.21	0.063	0.832
2	5hb4B	0.616	4.47	0.081	0.859
3	4knhA	0.612	4.40	0.052	0.828
4	4xriA2	0.595	3.43	0.053	0.738
5	3msvA	0.595	4.70	0.079	0.856
6	1qgkA	0.592	3.53	0.084	0.742
7	1wa5C	0.591	4.01	0.104	0.789
8	4ifqA	0.587	4.73	0.083	0.840
9	2x1gF	0.587	3.92	0.090	0.781
10	4pj3A	0.584	4.62	0.049	0.820

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.10	5	5e6qB	III	Rep, Mult	40,97,101,105,107,108,109,114,118,158,162,166,169,170,214,217,221,225
2	0.06	3	3kndA	III	Rep, Mult	42,108,114,118,162,168,169,170,180,214,217,220,221
3	0.06	3	2bapA	III	Rep, Mult	38,43,101,102,104,105,108,109,162,169,210,213,214,217
4	0.04	2	3m6zA	MG	Rep, Mult	195,199
5	0.02	1	1u6g0	III	Rep, Mult	91,92,96,145,156,157
6	0.02	1	1xi2A	CB1	Rep, Mult	130,191
7	0.02	1	2o012	CLA	Rep, Mult	172,173
8	0.02	1	3cu8B	MG	Rep, Mult	101,214
9	0.02	1	1iw7P	MG	Rep, Mult	137,141
10	0.02	1	1ya8B	SMB	Rep, Mult	86,148
11	0.02	1	1o6pB	III	Rep, Mult	157,161,214,217,218
12	0.02	1	4djdF	B12	Rep, Mult	193,194
13	0.02	1	1t080	III	Rep, Mult	166,169,213,214,216,220,221,224
14	0.02	1	1v87A	ZN	Rep, Mult	17,22,48,51
15	0.02	1	2i76A	NDP	Rep, Mult	210,231
16	0.02	1	2bgwA	MG	Rep, Mult	53,89,90

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1h12A	0.473	6.07	0.036	0.793	3.2.1.8	108
2	0.060	3gzkA	0.475	5.12	0.077	0.715	3.2.1.4	NA
3	0.060	1js4A	0.510	4.83	0.055	0.738	3.2.1.4	NA
4	0.060	1hcuB	0.526	4.96	0.062	0.750	3.2.1.113	NA
5	0.060	1fo2A	0.536	4.69	0.072	0.738	3.2.1.113	95,154
6	0.060	2q1fA	0.500	4.86	0.069	0.731	4.2.2.21	NA
7	0.060	1ojnA	0.527	4.77	0.026	0.754	4.2.2.1	NA
8	0.060	1f1sA	0.523	4.59	0.035	0.742	4.2.2.1	NA
9	0.060	1ug9A	0.504	5.19	0.081	0.762	3.2.1.70	NA
10	0.060	2qnoA	0.500	5.46	0.064	0.781	3.2.1.4	85
11	0.060	1gaiA	0.539	4.99	0.055	0.797	3.2.1.3	NA
12	0.060	1ut9A	0.476	5.74	0.057	0.777	3.2.1.4	NA
13	0.060	1g87B	0.503	5.44	0.053	0.781	3.2.1.4	NA
14	0.060	1dl2A	0.526	4.86	0.051	0.754	3.2.1.113	NA
15	0.060	1ft1B	0.486	4.82	0.037	0.711	2.5.1.58,2.5.1.-	NA
16	0.060	2eabA	0.526	5.09	0.065	0.766	3.2.1.63	NA
17	0.060	3c5wA	0.473	4.49	0.064	0.668	3.1.3.16	NA
18	0.060	1i8qA	0.525	4.58	0.043	0.742	4.2.2.1	NA
19	0.060	1w6jA	0.515	5.24	0.050	0.785	5.4.99.7	180

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.12	0.625	4.21	0.06	0.83	4kf7A	GO:0005643 GO:0006406 GO:0006606 GO:0006999 GO:0017056 GO:0031990 GO:0044611
1	0.07	0.581	4.72	0.08	0.83	4ifqA	GO:0000059 GO:0005634 GO:0005643 GO:0006810 GO:0006999 GO:0015031 GO:0017056 GO:0044611 GO:0046822 GO:0051028
2	0.07	0.610	4.22	0.06	0.82	4knhB	GO:0005634 GO:0005643 GO:0006810 GO:0015031 GO:0051028
3	0.07	0.612	4.40	0.05	0.83	4knhA	GO:0005634 GO:0005643 GO:0006810 GO:0015031 GO:0051028
4	0.07	0.616	4.47	0.08	0.86	5hb4B	GO:0005634 GO:0005643 GO:0006810 GO:0015031 GO:0051028
5	0.07	0.564	4.35	0.09	0.77	5ijnD	GO:0000059 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0006406 GO:0006409 GO:0006810 GO:0006913 GO:0007077 GO:0010827 GO:0015031 GO:0016020 GO:0016032 GO:0016925 GO:0017056 GO:0019083 GO:0031047 GO:0031965 GO:0034399 GO:0044611 GO:0051028 GO:0051292 GO:0075733 GO:1900034
6	0.07	0.494	4.07	0.07	0.68	4f7rD	GO:0019904
7	0.06	0.398	5.89	0.07	0.68	1n1zA	GO:0000287 GO:0008152 GO:0009507 GO:0009536 GO:0010333 GO:0016829 GO:0016853 GO:0046211 GO:0046872 GO:0047926
8	0.06	0.329	6.36	0.03	0.59	4dozA	GO:0000166 GO:0003723 GO:0005737 GO:0046872 GO:0051607
9	0.06	0.357	5.95	0.04	0.60	1bagA	GO:0003824 GO:0004556 GO:0005576 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0043169 GO:0046872
10	0.06	0.337	5.44	0.04	0.53	1w62A	GO:0005576 GO:0005737 GO:0007275 GO:0016020 GO:0016853 GO:0018112
11	0.06	0.300	5.48	0.03	0.47	4hs7A	GO:0005363 GO:0015768
12	0.06	0.291	5.78	0.07	0.47	4dwjB	GO:0000166 GO:0004798 GO:0005524 GO:0006233 GO:0006235 GO:0009165 GO:0016301 GO:0016310 GO:0016740 GO:0046939
13	0.06	0.307	5.87	0.05	0.51	4q0mA	GO:0004067 GO:0006520
14	0.06	0.320	6.37	0.06	0.57	1k9xA	GO:0004180 GO:0004181 GO:0006508 GO:0008233 GO:0008237 GO:0016787 GO:0046872 GO:0050897
15	0.06	0.293	6.86	0.04	0.57	3wxeA	GO:0004843 GO:0005829 GO:0006511 GO:0007346 GO:0016579 GO:0021551 GO:0032088 GO:0036459 GO:0061578 GO:0070266 GO:0070536 GO:1990108 GO:2001238 GO:2001242
16	0.06	0.291	3.59	0.02	0.37	1xlyB	GO:0003723 GO:0003729 GO:0005634 GO:0005737 GO:0005789 GO:0005934 GO:0006810 GO:0007533 GO:0008289 GO:0008298 GO:0051028
17	0.06	0.260	6.53	0.07	0.47	1uamA	GO:0005737 GO:0006400 GO:0008033 GO:0008168 GO:0008685 GO:0009019 GO:0016740 GO:0030488 GO:0032259 GO:0052906
18	0.06	0.236	5.07	0.04	0.36	4g3kA	GO:0000166 GO:0003677 GO:0005524 GO:0006351 GO:0006355 GO:0008134 GO:0043565

Consensus prediction of GO terms

Molecular Function	GO:0005198
GO-Score	0.37
Biological Processes	GO:0050658	GO:0045184	GO:0006997
GO-Score	0.48	0.48	0.37
Cellular Component	GO:0043234	GO:0005643
GO-Score	0.37	0.34

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.