I-TASSER results

I-TASSER results for job id Rv0074

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (411 residues)
MGDLSISQVSARPGRIGIRARQMFDGYRFQRGPVLVVVEDGRISAVDFAGSACPDMNLVD
LGESTLLPGLVDAHAHLCWDPDGRPEDLAGDPHAVLVGRARRHAAAALRSGITTIRDLGD
RDYAALALREEYRQKTTVGPELVVSGPPLTRSGGHCWFLGGVADSVEELVDAVQERAARG
ADWIKVMATGGFVTTASDPWQPQYGSGQLAAVVAAAEQVGLPVTAHAHATAGIAAAVAAG
VDGIEHCTFLSEGSAAASPDVVEAIVAQGVWCGMTIPRVYPEMPENLVAVVQDGWRNIRR
LIDAGARVALSTDAGVAPGRRHDVLPDDLVYLSRHGFTSTEVLTGATAAAAASCGLGHRK
GRIAPGYDADLLAVAAGVDHDPAGLCDVKAVWRSGTQVPLQASAVGYNTPS

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MGDLSISQVSARPGRIGIRARQMFDGYRFQRGPVLVVVEDGRISAVDFAGSACPDMNLVDLGESTLLPGLVDAHAHLCWDPDGRPEDLAGDPHAVLVGRARRHAAAALRSGITTIRDLGDRDYAALALREEYRQKTTVGPELVVSGPPLTRSGGHCWFLGGVADSVEELVDAVQERAARGADWIKVMATGGFVTTASDPWQPQYGSGQLAAVVAAAEQVGLPVTAHAHATAGIAAAVAAGVDGIEHCTFLSEGSAAASPDVVEAIVAQGVWCGMTIPRVYPEMPENLVAVVQDGWRNIRRLIDAGARVALSTDAGVAPGRRHDVLPDDLVYLSRHGFTSTEVLTGATAAAAASCGLGHRKGRIAPGYDADLLAVAAGVDHDPAGLCDVKAVWRSGTQVPLQASAVGYNTPS
Prediction	CCCCCCCCCCCCCCCEEEEEEEEHCCCCCCCCCEEEEEHCCEEEEEHCCCCCCCCCEEEHCCCCEEHCCCEHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEHCCCCCCHHHHHHHHHHHCCCCCCEEEHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHCCHHHHHHHHHHCCCEEEHCCCCCHHHHHHHHHHHHHHHCCCEEEEHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEHCCCEHCCEEEEHCCCCCCHHHHCCCCEEEHCCEEEHCCCCCCCCCCCC
Conf.Score	998887777788885899956998899886688699999999999858888899985998699689558633455665689987477658999999999999999999989988995799985699999999989998987998476657788888888888899999999999999989998999986887778888888889999999999999999997999857899999999999987874667898899987887666435785897556668988999999999999999999999899799967889988887589999999999989999999999999999998998986554897767599995996559788578869999989998678877788899
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MGDLSISQVSARPGRIGIRARQMFDGYRFQRGPVLVVVEDGRISAVDFAGSACPDMNLVDLGESTLLPGLVDAHAHLCWDPDGRPEDLAGDPHAVLVGRARRHAAAALRSGITTIRDLGDRDYAALALREEYRQKTTVGPELVVSGPPLTRSGGHCWFLGGVADSVEELVDAVQERAARGADWIKVMATGGFVTTASDPWQPQYGSGQLAAVVAAAEQVGLPVTAHAHATAGIAAAVAAGVDGIEHCTFLSEGSAAASPDVVEAIVAQGVWCGMTIPRVYPEMPENLVAVVQDGWRNIRRLIDAGARVALSTDAGVAPGRRHDVLPDDLVYLSRHGFTSTEVLTGATAAAAASCGLGHRKGRIAPGYDADLLAVAAGVDHDPAGLCDVKAVWRSGTQVPLQASAVGYNTPS
Prediction	613132433424333001210414216433365012003662312106546226503101034243741514010000000212223234232110122014103300410000000001332102101411653312001000002000211110222322121022004000500441020000000101012224122000224002100400372713000001125002200410000000001014300410450031003310000000130222124303411540150032016140200000000002122110001001001401000110020011100200415531010104230000001001240052053030002103102264551436268
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCEEEEEEEEHCCCCCCCCCEEEEEHCCEEEEEHCCCCCCCCCEEEHCCCCEEHCCCEHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEHCCCCCCHHHHHHHHHHHCCCCCCEEEHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHCCHHHHHHHHHHCCCEEEHCCCCCHHHHHHHHHHHHHHHCCCEEEEHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEHCCCEHCCEEEEHCCCCCCHHHHCCCCEEEHCCEEEHCCCCCCCCCCCC
MGDLSISQVSARPGRIGIRARQMFDGYRFQRGPVLVVVEDGRISAVDFAGSACPDMNLVDLGESTLLPGLVDAHAHLCWDPDGRPEDLAGDPHAVLVGRARRHAAAALRSGITTIRDLGDRDYAALALREEYRQKTTVGPELVVSGPPLTRSGGHCWFLGGVADSVEELVDAVQERAARGADWIKVMATGGFVTTASDPWQPQYGSGQLAAVVAAAEQVGLPVTAHAHATAGIAAAVAAGVDGIEHCTFLSEGSAAASPDVVEAIVAQGVWCGMTIPRVYPEMPENLVAVVQDGWRNIRRLIDAGARVALSTDAGVAPGRRHDVLPDDLVYLSRHGFTSTEVLTGATAAAAASCGLGHRKGRIAPGYDADLLAVAAGVDHDPAGLCDVKAVWRSGTQVPLQASAVGYNTPS

3be7A

0.26

0.24

0.93

2.72

MUSTER

------------SEDFLIKSKGYLDIQTGEIIKADLLIRNGKIAEIGKIN--TKDATVISIPDLILIPGLMDSHVHIVGNDSKGEES-IADSSHMGTVWGVVNAEKTLMAGFTTVRNVGAANYADVSVRDAIERGVINGPTMLVSGPALGITGGHCDHNEGVVDSPWEARKMVRKNRKYGADLIKFCATGGVMSRNTDVNAKQFTLEEMKAIVDEAHNHGMKVAAHAHGLIGIKAAIKAGVDSVEHASFIDDETIDMAIKNNTVLSMDIFVSDYILAGIREESLNKERLVGKKQRENFMNAHRRGAIITFGTDAGIFD---HGDNAKQFAYMVEWGMTPLEAIQASTIKTATLFGIEN-IGQIKEGFDADIVGVIENPLANIRTLEEVAFVMKEGKVYKREG---------

3be7A

0.25

0.24

0.93

3.20

dPPAS

------------SEDFLIKSKGYLDIQTGEIIKADLLIRNGKIAEIGKIN--TKDATVISIPDLILIPGLMDSHVHIVGNDSKGEES-IADSSHMGTVWGVVNAEKTLMAGFTTVRNVGAANYADVSVRDAIERGVINGPTMLVSGPALGITGGHCDHNLLPVDSPWEARKMVRKNRKYGADLIKFCATGGVMSRNTDVNAKQFTLEEMKAIVDEAHNHGMKVAAHAHGLIGIKAAIKAGVDSVEHASFIDDETIDMAIKNNTVLSMDIFVSDYAKAGIREESLNKERLVGKKQRENFMNAHRRGAIITFGTDAGIFDHGD---NAKQFAYMVEWGMTPLEAIQASTIKTATLFGIEN-IGQIKEGFDADIVGVIENPLANIRTLEEVAFVMKEGKVYKREG---------

3be7A

0.26

0.24

0.93

3.96

wdPPAS

------------SEDFLIKSKGYLDIQTGEIIKADLLIRNGKIAEIGKIN--TKDATVISIPDLILIPGLMDSHVHIVGNDSKGEES-IADSSHMGTVWGVVNAEKTLMAGFTTVRNVGAANYADVSVRDAIERGVINGPTMLVSGPALGITGGHCDHNEGVVDSPWEARKMVRKNRKYGADLIKFCATGGVMSRNTDVNAKQFTLEEMKAIVDEAHNHGMKVAAHAHGLIGIKAAIKAGVDSVEHASFIDDETIDMAIKNNTVLSMDIFVSDYAKAGIREESLNKERLVGKKQRENFMNAHRRGAIITFGTDAGIFD---HGDNAKQFAYMVEWGMTPLEAIQASTIKTATLFGIEN-IGQIKEGFDADIVGVIENPLANIRTLEEVAFVMKEGKVYKREG---------

3be7A

0.26

0.24

0.93

3.58

wMUSTER

-------------EDFLIKSKGYLDIQTGEIIKADLLIRNGKIAEIGKIN--TKDATVISIPDLILIPGLMDSHVHIVGNDSKGEES-IADSSHMGTVWGVVNAEKTLMAGFTTVRNVGAANYADVSVRDAIERGVINGPTMLVSGPALGITGGHCDHNEGVVDSPWEARKMVRKNRKYGADLIKFCATGGVMSRNTDVNAKQFTLEEMKAIVDEAHNHGMKVAAHAHGLIGIKAAIKAGVDSVEHASFIDDETIDMAIKNNTVLSMDIFVSDYILAGIREESLNKERLVGKKQRENFMNAHRRGAIITFGTDAGIFD---HGDNAKQFAYMVEWGMTPLEAIQASTIKTATLFGIEN-IGQIKEGFDADIVGVIENPLANIRTLEEVAFVMKEGKVYKREG---------

3be7A

0.26

0.24

0.93

4.12

wPPAS

------------SEDFLIKSKGYLDIQTGEIIKADLLIRNGKIAEIGKIN--TKDATVISIPDLILIPGLMDSHVHIVGNDSKGEES-IADSSHMGTVWGVVNAEKTLMAGFTTVRNVGAANYADVSVRDAIERGVINGPTMLVSGPALGITGGHCDHNEGVVDSPWEARKMVRKNRKYGADLIKFCATGGVMSRNTDVNAKQFTLEEMKAIVDEAHNHGMKVAAHAHGLIGIKAAIKAGVDSVEHASFIDDETIDMAIKNNTVLSMDIFVSDYAKAGIREESLNKERLVGKKQRENFMNAHRRGAIITFGTDAGIFD---HGDNAKQFAYMVEWGMTPLEAIQASTIKTATLFGIEN-IGQIKEGFDADIVGVIENPLANIRTLEEVAFVMKEGKVYKREG---------

3be7A

0.26

0.24

0.93

5.22

dPPAS2

------------SEDFLIKSKGYLDIQTGEIIKADLLIRNGKIAEIGKIN--TKDATVISIPDLILIPGLMDSHVHIVGNDSKGEES-IADSSHMGTVWGVVNAEKTLMAGFTTVRNVGAANYADVSVRDAIERGVINGPTMLVSGPALGITGGHCDHNEGVVDSPWEARKMVRKNRKYGADLIKFCATGGVMSRNTDVNAKQFTLEEMKAIVDEAHNHGMKVAAHAHGLIGIKAAIKAGVDSVEHASFIDDETIDMAIKNNTVLSMDIFVSDYAKAGIREESLNKERLVGKKQRENFMNAHRRGAIITFGTDAGIFD---HGDNAKQFAYMVEWGMTPLEAIQASTIKTATLFGIEN-IGQIKEGFDADIVGVIENPLANIRTLEEVAFVMKEGKVYKREG---------

3be7A

0.26

0.24

0.93

3.97

PPAS

------------SEDFLIKSKGYLDIQTGEIIKADLLIRNGKIAEIGKIN--TKDATVISIPDLILIPGLMDSHVHIVGNDSKGEES-IADSSHMGTVWGVVNAEKTLMAGFTTVRNVGAANYADVSVRDAIERGVINGPTMLVSGPALGITGGHCDHNEGVVDSPWEARKMVRKNRKYGADLIKFCATGGVMSRNTDVNAKQFTLEEMKAIVDEAHNHGMKVAAHAHGLIGIKAAIKAGVDSVEHASFIDDETIDMAIKNNTVLSMDIFVSDYAKAGIREESLNKERLVGKKQRENFMNAHRRGAIITFGTDAGIFD---HGDNAKQFAYMVEWGMTPLEAIQASTIKTATLFGIEN-IGQIKEGFDADIVGVIENPLANIRTLEEVAFVMKEGKVYKREG---------

3be7A

0.26

0.24

0.93

5.04

Env-PPAS

------------SEDFLIKSKGYLDIQTGEIIKADLLIRNGKIAEIGKIN--TKDATVISIPDLILIPGLMDSHVHIVGNDSKGEE-SIADSSHMGTVWGVVNAEKTLMAGFTTVRNVGAANYADVSVRDAIERGVINGPTMLVSGPALGITGGHCDHNEGVVDSPWEARKMVRKNRKYGADLIKFCATGGVMSRNTDVNAKQFTLEEMKAIVDEAHNHGMKVAAHAHGLIGIKAAIKAGVDSVEHASFIDDETIDMAIKNNTVLSMDIFVSDYAKAGIREESLNKERLVGKKQRENFMNAHRRGAIITFGTDAGIFD---HGDNAKQFAYMVEWGMTPLEAIQASTIKTATLFGIEN-IGQIKEGFDADIVGVIENPLANIRTLEEVAFVMKEGKVYKREG---------

3gnhA

0.28

0.26

0.93

2.71

MUSTER

-------------EIKAVSAARLLDVASGYVDNPLVIVTDGRITSIGKKGDVPAGATAVDLPGVTLLPGLIDMHVHLDSLAEVGGYNSLEYSDRFWSVVQTANAKKTLEAGFTTVRNVGAADYDDVGLREAIDAGYVPGPRIVTAAISFGATGGHCDSTFFNSDSPDEARKAVRTLKKYGAQVIICAT-GGVFSRGNEPGQQQLTYEEMKAVVDEAHMAGIKVAAHAHGASGIREAVRAGVDTIEHASLVDDEGIKLAVQKGAYFSMDIYNTDYTQNGVLEDNLRKDRDIGELQRENFRKALKAGVKMVYGTDAGIYP---HGDNAKQFAVMVRYGATPLQAIQSATLTAAEALGRSKDVGQVAVGRYGDMIAVAGDPLADVTTLEKPVFVMKGGAVVKA-----------

3gnhA

0.28

0.26

0.93

3.17

dPPAS

-------------EIKAVSAARLLDVASGYVDNPLVIVTDGRITSIGKKGDVPAGATAVDLPGVTLLPGLIDMHVHLDSLAEVGGYNSLEYSDRFWSVVQTANAKKTLEAGFTTVRNVGAADYDDVGLREAIDAGYVPGPRIVTAAISFGATGGHCDSTPFNSDSPDEARKAVRTLKKYGAQVIICAT-GGVFSRGNEPGQQQLTYEEMKAVVDEAHMAGIKVAAHAHGASGIREAVRAGVDTIEHASLVDDEGIKLAVQKGAYFSMDIYNTDYTQAGVLEDNLRKDRDIGELQRENFRKALKAGVKMVYGTDAGIYPHGD---NAKQFAVMVRYGATPLQAIQSATLTAAEALGRSKDVGQVAVGRYGDMIAVAGDPLADVTTLEKPVFVMKGGAVVKA-----------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.14

Estimated TM-score = 0.87±0.07

Estimated RMSD = 4.5±3.0Å

Download Model 2

C-score=-3.38

Download Model 3

C-score=-3.72

Download Model 4

C-score=-3.79

Download Model 5

C-score=-4.73

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3be7A	0.914	1.08	0.261	0.932
2	3gnhA	0.896	1.62	0.285	0.932
3	2r8cA	0.878	2.08	0.281	0.927
4	3feqN	0.877	2.00	0.266	0.922
5	4c5yA	0.874	2.36	0.262	0.947
6	2qs8A	0.858	1.90	0.285	0.905
7	4whbE	0.838	3.10	0.203	0.937
8	4wgxA	0.838	3.04	0.189	0.942
9	2p9bA	0.814	2.51	0.274	0.883
10	3ls9A	0.765	3.20	0.164	0.874

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.77	191	3dc8A	ZN	Rep, Mult	74,76,185,313
2	0.09	24	3n2cD	LWY	Rep, Mult	74,76,155,185,192,193,226,228,246,272,275,313,315
3	0.08	18	4c5yA	ZN	Rep, Mult	74,185,226,246
4	0.05	11	3be7E	ARG	Rep, Mult	155,191,192,193,226,228,246,268,271,275,287,290,291,294,313,316
5	0.01	3	3mkvF	CO3	Rep, Mult	155,192,193,228,313
6	0.01	2	3feqN	ZN	Rep, Mult	155,226,246
7	0.01	2	3be7F	MG	Rep, Mult	141,394
8	0.00	1	3dugA	ZN	Rep, Mult	302,341
9	0.00	1	3o7uA	PXN	Rep, Mult	98,101,102,105,130

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.283	3egjA	0.670	3.56	0.180	0.793	3.5.1.25	309
2	0.245	2qt3A	0.731	3.13	0.148	0.837	3.5.99.4	68,363
3	0.211	2vhlB	0.681	3.63	0.180	0.810	3.5.1.25	NA
4	0.199	2uz9A	0.735	3.38	0.159	0.854	3.5.4.3	112,133
5	0.195	1xrtA	0.677	3.37	0.156	0.786	3.5.2.3	NA
6	0.193	2g3fA	0.754	3.37	0.162	0.866	3.5.2.7	NA
7	0.187	2puzA	0.742	3.49	0.217	0.861	3.5.2.7	NA
8	0.122	2gokA	0.741	3.50	0.214	0.861	3.5.2.7	313
9	0.117	3iv8D	0.673	3.50	0.180	0.793	3.5.1.25	217
10	0.114	1k6wA	0.735	3.43	0.169	0.854	3.5.4.1	NA
11	0.112	1ra0A	0.731	3.50	0.172	0.854	3.5.4.1	NA
12	0.107	2i9uA	0.716	3.34	0.146	0.832	3.5.4.3	NA
13	0.070	1zzmA	0.494	3.37	0.162	0.579	3.1.21.-	312
14	0.060	1ie7C	0.701	3.41	0.142	0.818	3.5.1.5	NA
15	0.060	2ftwA	0.694	4.21	0.153	0.859	3.5.2.2	NA
16	0.060	3e0lA	0.733	3.36	0.163	0.852	3.5.4.3	NA
17	0.060	1a5kC	0.706	3.34	0.159	0.820	3.5.1.5	NA
18	0.060	1e9yB	0.701	3.41	0.127	0.818	3.5.1.5	NA
19	0.060	2ftyA	0.697	4.45	0.153	0.876	3.5.2.2	NA
20	0.060	3griB	0.679	3.74	0.142	0.803	3.5.2.3	171
21	0.060	3d6nA	0.702	3.58	0.152	0.825	3.5.2.3	312,313
22	0.060	1nfgA	0.695	4.15	0.161	0.849	3.5.2.2	NA
23	0.060	1m7jA	0.723	4.19	0.158	0.886	3.5.1.81	313
24	0.060	1p1mA	0.728	3.09	0.173	0.825	3.5.4.28	NA
25	0.060	3giqA	0.721	3.92	0.160	0.874	3.5.1.82	25,348,351,355
26	0.060	2vunD	0.691	3.24	0.184	0.793	3.5.2.18	107
27	0.060	1k1dA	0.700	4.08	0.157	0.854	3.5.2.-	313
28	0.060	1onxA	0.692	3.69	0.190	0.815	3.4.19.-	313
29	0.060	1kraC	0.706	3.34	0.162	0.820	3.5.1.5	332
30	0.060	2oodA	0.729	3.29	0.170	0.842	3.5.4.3	NA
31	0.060	2p50A	0.674	3.63	0.191	0.800	3.5.1.25	NA
32	0.060	2qt3B	0.730	3.14	0.145	0.837	3.5.99.4	68,363

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.56	0.914	1.08	0.26	0.93	3be7A
1	0.56	0.879	2.09	0.28	0.93	3mkvA	GO:0016787 GO:0016810 GO:0046872
2	0.55	0.887	1.88	0.28	0.93	2qs8A	GO:0016787 GO:0016810 GO:0046872
3	0.54	0.877	2.00	0.27	0.92	3n2cA
4	0.47	0.742	3.01	0.15	0.84	3hpaA
5	0.45	0.693	3.76	0.16	0.83	3hm7B	GO:0000256 GO:0004038 GO:0006144 GO:0008270 GO:0016787 GO:0016810 GO:0016812 GO:0046872 GO:0050897
6	0.44	0.674	3.98	0.16	0.82	3e74B	GO:0000256 GO:0004038 GO:0005737 GO:0006144 GO:0008270 GO:0009442 GO:0016787 GO:0016810 GO:0046872 GO:0050897
7	0.39	0.692	3.70	0.19	0.82	1ybqA	GO:0005737 GO:0005829 GO:0006508 GO:0008233 GO:0008237 GO:0008270 GO:0008798 GO:0016787 GO:0016810 GO:0046872
8	0.39	0.903	1.64	0.28	0.94	3mtwA	GO:0016787 GO:0016810 GO:0046872
9	0.38	0.865	2.70	0.26	0.95	4c60A	GO:0006508 GO:0016787 GO:0016805 GO:0016810 GO:0046872 GO:0102009 GO:1900817
10	0.38	0.837	3.15	0.21	0.94	4whbA	GO:0016787 GO:0016810
11	0.37	0.684	4.26	0.14	0.85	3dc8A	GO:0005737 GO:0016787 GO:0016810 GO:0046872
12	0.35	0.720	3.92	0.16	0.87	3gipA	GO:0016787 GO:0016810 GO:0016811 GO:0047421
13	0.35	0.833	2.52	0.27	0.91	2p9bA	GO:0016787 GO:0016810
14	0.32	0.837	3.11	0.18	0.94	4ub9A	GO:0016787 GO:0016810
15	0.31	0.691	3.24	0.18	0.79	2vunA	GO:0016787 GO:0016810 GO:0043792 GO:0046872 GO:0051187
16	0.29	0.699	4.11	0.16	0.85	3sfwA	GO:0005737 GO:0016787 GO:0016810 GO:0046872
17	0.29	0.706	3.49	0.20	0.82	3nqbA	GO:0000034 GO:0006146 GO:0016787 GO:0016810
18	0.28	0.700	4.08	0.16	0.85	1k1dA	GO:0005737 GO:0016787 GO:0016810 GO:0046872
19	0.24	0.655	3.94	0.22	0.78	3ooqF	GO:0016787 GO:0016810 GO:0019239
20	0.20	0.703	3.41	0.13	0.82	4z42C	GO:0005737 GO:0006807 GO:0009039 GO:0016151 GO:0016787 GO:0016810 GO:0019627 GO:0043419 GO:0046872
21	0.15	0.770	3.12	0.18	0.88	4l9xB	GO:0004040 GO:0016787 GO:0016810 GO:0046872
22	0.13	0.756	3.09	0.19	0.86	4dzhA	GO:0004000 GO:0016787 GO:0016810 GO:0019700 GO:0046872 GO:0050270
23	0.13	0.723	4.19	0.16	0.89	1m7jA	GO:0016787 GO:0016810 GO:0016811 GO:0046872 GO:0047420
24	0.12	0.696	4.16	0.17	0.85	1nfgA	GO:0005737 GO:0016787 GO:0016810 GO:0046872
25	0.11	0.696	4.21	0.15	0.86	2ftwA	GO:0004157 GO:0005615 GO:0005737 GO:0006208 GO:0008270 GO:0016787 GO:0016810 GO:0046872
26	0.11	0.677	3.37	0.16	0.79	1xrtA	GO:0004151 GO:0006221 GO:0008270 GO:0016787 GO:0016810 GO:0016812 GO:0044205 GO:0046872
27	0.11	0.721	3.35	0.15	0.83	3ighX	GO:0006259 GO:0016810 GO:0016888
28	0.10	0.702	3.77	0.14	0.83	4bjhA	GO:0004151 GO:0006221 GO:0008270 GO:0016787 GO:0016810 GO:0016812 GO:0044205 GO:0046872
29	0.10	0.696	3.88	0.15	0.83	3mpgA	GO:0004151 GO:0006221 GO:0008270 GO:0009220 GO:0016787 GO:0016810 GO:0016812 GO:0044205 GO:0046872
30	0.08	0.696	4.14	0.16	0.85	1gkrA	GO:0000256 GO:0004038 GO:0008270 GO:0016787 GO:0016810 GO:0046872 GO:0050897
31	0.08	0.705	3.32	0.15	0.81	3icjA	GO:0006259 GO:0016810 GO:0016888 GO:0046872
32	0.08	0.689	4.10	0.14	0.84	4tqtD	GO:0004157 GO:0005737 GO:0016787 GO:0016810

Consensus prediction of GO terms

Molecular Function	GO:0016810	GO:0046872
GO-Score	0.80	0.80
Biological Processes
GO-Score
Cellular Component
GO-Score

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.