I-TASSER results

I-TASSER results for job id Rv0059

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (230 residues)
MITRYKPESGFVARSGGPDRKRPHDWIVWHFTHADNLPGIITAGRLLADSAVTPTTEVAY
NPVKELRRHKVVAPDSRYPASMASDHVPFYIAARSPMLYVVCKGHSGYSGGAGPLVHLGV
ALGDIIDADLTWCASDGNAAASYTKFSRQVDTLGTFVDFDLLCQRQWHNTDDDPNRQSRR
AAEILVYGHVPFELVSYVCCYNTETMTRVRTLLDPVGGVRKYVIKPGMYY

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MITRYKPESGFVARSGGPDRKRPHDWIVWHFTHADNLPGIITAGRLLADSAVTPTTEVAYNPVKELRRHKVVAPDSRYPASMASDHVPFYIAARSPMLYVVCKGHSGYSGGAGPLVHLGVALGDIIDADLTWCASDGNAAASYTKFSRQVDTLGTFVDFDLLCQRQWHNTDDDPNRQSRRAAEILVYGHVPFELVSYVCCYNTETMTRVRTLLDPVGGVRKYVIKPGMYY
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCHCCCCCCCCCCEEEEHCCCCCCCCCCCCEEEEEHCHHHHHHHCCCEEEHCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHCEEEEEHCCCCCHHHCEEEEHCCHHHHHHHHHHHHHHCCCCEEEHCCCCCC
Conf.Score	98776777775456888888888887699998636799999989975444567887667756899999866666677899854544456557898435676537777777888789999858998987998899789887776455479889886466777764467788877554544456887589889777357666899999999999987599667998898889
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MITRYKPESGFVARSGGPDRKRPHDWIVWHFTHADNLPGIITAGRLLADSAVTPTTEVAYNPVKELRRHKVVAPDSRYPASMASDHVPFYIAARSPMLYVVCKGHSGYSGGAGPLVHLGVALGDIIDADLTWCASDGNAAASYTKFSRQVDTLGTFVDFDLLCQRQWHNTDDDPNRQSRRAAEILVYGHVPFELVSYVCCYNTETMTRVRTLLDPVGGVRKYVIKPGMYY
Prediction	75343334431143343575534551200000115203200630101132433653403355145324454345243344130100000101141000010244344364552300001030430245733000000111342242144165045303151144530543663463253100000036402140021000216611530351057471513030347127
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCHCCCCCCCCCCEEEEHCCCCCCCCCCCCEEEEEHCHHHHHHHCCCEEEHCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHCEEEEEHCCCCCHHHCEEEEHCCHHHHHHHHHHHHHHCCCCEEEHCCCCCC
MITRYKPESGFVARSGGPDRKRPHDWIVWHFTHADNLPGIITAGRLLADSAVTPTTEVAYNPVKELRRHKVVAPDSRYPASMASDHVPFYIAARSPMLYVVCKGHSGYSGGAGPLVHLGVALGDIIDADLTWCASDGNAAASYTKFSRQVDTLGTFVDFDLLCQRQWHNTDDDPNRQSRRAAEILVYGHVPFELVSYVCCYNTETMTRVRTLLDPVGGVRKYVIKPGMYY

5iitA2

0.07

0.06

0.82

0.62

MUSTER

YDIARTSGAGSDGFTVLSTKSLFLGQKLQVVQ---DIASIDSDAVVHPTNTDFYIGGEVGNTLEKKGGKEFVENGPLEVAGAA--VSAGHGLPAKFVIHCNSPVWGADKCEELLEKTVKNCLALADDKKLKSIAFP---SIGSGRNGFPKQTAAQLI-LKAISS-YFVSTMSS---------------SIKT---VYFVLFDSESIGIYVQEMAKL--------------

5flzC2

0.10

0.96

0.78

dPPAS

------TYSVFPARSSEVVRRLIEDSDATVVFDNASLLNISGKVF---RNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKCHSSYDVMLDLLDPSNSLVSTAMNNPTYNVEPRQISRAMTKLQQRIKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYN

5dazA

0.17

0.11

0.63

0.73

wdPPAS

VHAAADPRV-DVERITPDPVWRTTCGTLYRSDS-RGPAVVFEQGFLPKDVID------GQYDIESYVLVNQPSPVSTTYDHDLYK------WYKSGYNYYIDAPGG-------------VDVNKTIGDRHKW----------------------------------------------ADQVEVAFPGGIRTEFVIGVCPVDKKTRTEKMSEC---------VGNPH--Y

5iitA2

0.09

0.07

0.80

0.58

wMUSTER

YDIARTSGAGSDGFTVLSTKSLFLGQQVVQIASID-SDAVV----HPTNTDFYIGGEVGGKEFVEAVLELRKKNGPLEVAGAA--VSAGHGLPAKFVIHCNSPVWGADKCEELLEKTVKNCLALADDKKLKSIAFPS-IGSGRNGF--PKQTAAQLI-LKAISS-YFVSTMSSS--------------------IKTVYF-DSESIGIYVQEMAKL--------------

5dazA

0.19

0.11

0.60

0.68

wPPAS

-AKAAAPACPFVHAAADPRVDRTTCGTLYRSDS-RGPAVVFEQG-FLPKDVIDGQYDI-ESYVLVN-----------QPSP----YV--TW-YKSGYNYYIDAPGG-------------VDVNKTIGDRHKW----------------------------------------------ADQVEVAFPGGIRTEFVIGVCPVDKKTRTEKMSEC---------VGNPH-YW

2k3cA

0.24

0.03

0.11

0.80

dPPAS2

SYMAYLSASGIMALIASGVVMRPKK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

5flzC2

0.11

0.10

0.96

0.81

PPAS

---TY---SVFPARSSEV-RRLIEDSDATVVFDNASLLNISGKVFRNPN---IDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKCHSSYDVMLDLLDPSNSLVSTAMNNPTYNVEPRQISRAMTKLQQRIKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYN

1wfxA2

0.21

0.09

0.41

0.71

Env-PPAS

-----------------PLPGEP-PPILYHGTTEEALP-LIERGIRGRRL-----------------------------------KV--HLTSSLEDAVSTGRRH------GNLVAVLLVDVECLRRRGLKVERSKT---------------------------------------------VYTVD-WVPPECIAEVRRESL---------------------------

5flzC2

0.11

0.10

0.96

0.62

MUSTER

---TY---SVFPARSSEVVRRLIEDSDATVVFDNASLLNISGKVF---RNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKCHSSYDVMLDLLDPSNSLVSTAMNNPTYNVEPRQISRAMTKLQQRIKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYN

3utnX

0.10

0.99

0.77

dPPAS

LTKPRIPNSIFFDIDAISDKKSPYPHMF---PTKKVFDDAMSNLGVQKDDILYDRVGNFSSPRCAWTLGVMGHPKVYLLNNFNQYREFKYPLDSSKVAAFSPYPKSHYESSESFQDKEIVDYEEMFQLVRSLGRFEGTEPEPRSDIPSGHIPGTQPLPYGSLLDPETKTYPEAGEAIHATLEKALKDFHCTLDPSKPTICSTGVSGVIIKTALELAGVPNVRLYDGSWTE

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-2.55

Estimated TM-score = 0.42±0.14

Estimated RMSD = 11.5±4.5Å

Download Model 2

C-score=-3.66

Download Model 3

C-score=-4.95

Download Model 4

C-score=-5.00

Download Model 5

C-score=-4.33

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	5flzC	0.918	1.85	0.097	0.987
2	3cb2A	0.774	3.05	0.056	0.930
3	1tubA	0.772	3.36	0.063	0.961
4	3hkeD	0.772	3.21	0.064	0.957
5	2btoB	0.772	3.12	0.060	0.939
6	2btqB	0.722	3.39	0.053	0.904
7	3zidA	0.655	3.58	0.035	0.839
8	4b45A	0.629	3.49	0.065	0.804
9	4b46A	0.613	3.59	0.077	0.787
10	2r6r1	0.604	3.79	0.064	0.800

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.10	5	4x1iD	LOC	Rep, Mult	73,79,81,85,86,89,90,176,178,180,199
2	0.10	5	5ca0D	LXL	Rep, Mult	28,30,69,70,72,73,81,82,83,85,86,89,90,178,179,180
3	0.06	3	3hkdB	N16	Rep, Mult	3,8,28,30,69,70,73,83,86,90,178,199
4	0.04	2	2fhxB	ZN	Rep, Mult	24,30,105
5	0.02	1	3kvyA	UUU	Rep, Mult	30,94
6	0.02	1	1xqaA	MG	Rep, Mult	30,57
7	0.02	1	1f66C	MN	Rep, Mult	30,33
8	0.02	1	1l5yA	BF2	Rep, Mult	64,67
9	0.02	1	1gybA	III	Rep, Mult	159,226
10	0.02	1	2ih9B	5AX	Rep, Mult	30,44
11	0.02	1	3du7D	HOS	Rep, Mult	42,51,52,53,55
12	0.02	1	3hkeB	T13	Rep, Mult	72,76,77,78,182,183
13	0.02	1	2qqwA	ZN	Rep, Mult	33,35
14	0.02	1	3rphA	AMP	Rep, Mult	189,191,198
15	0.02	1	3t91A	MN	Rep, Mult	136,172

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2np0A	0.430	5.64	0.028	0.722	3.4.24.69	NA
2	0.060	3ikmB	0.410	5.65	0.077	0.704	2.7.7.7	NA
3	0.060	1adjA	0.440	5.75	0.048	0.748	6.1.1.21	NA
4	0.060	1nj8D	0.435	5.71	0.021	0.770	6.1.1.15	6,208
5	0.060	2j3lB	0.452	5.73	0.030	0.761	6.1.1.15	NA
6	0.060	1rm6A	0.401	6.49	0.057	0.756	1.3.99.20	NA
7	0.060	2zt5A	0.440	5.88	0.040	0.774	6.1.1.14	NA
8	0.060	2ftyA	0.427	5.17	0.024	0.661	3.5.2.2	NA
9	0.060	3ikmC	0.427	5.88	0.076	0.752	2.7.7.7	NA
10	0.060	2g4cA	0.426	5.77	0.071	0.748	2.7.7.7	NA
11	0.060	3errA	0.426	5.69	0.052	0.726	6.1.1.11	49
12	0.060	1sesA	0.425	5.81	0.041	0.735	6.1.1.11	127,137,147
13	0.060	1e1hD	0.228	4.29	0.018	0.313	3.4.24.69	NA
14	0.060	2etfB	0.412	5.62	0.034	0.687	3.4.24.69	201
15	0.060	1zkxB	0.406	5.55	0.030	0.700	3.4.24.69	NA
16	0.060	1qe0B	0.428	5.59	0.059	0.709	6.1.1.21	NA
17	0.060	1kmmC	0.434	5.73	0.075	0.744	6.1.1.21	NA
18	0.060	1nj1A	0.430	5.50	0.032	0.730	6.1.1.15	NA
19	0.060	1ogyI	0.426	5.49	0.086	0.700	1.7.99.4	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.22	0.918	1.85	0.10	0.99	5flzC	GO:0000166 GO:0000928 GO:0000930 GO:0003924 GO:0005200 GO:0005525 GO:0005634 GO:0005737 GO:0005816 GO:0005822 GO:0005824 GO:0005856 GO:0005874 GO:0007017 GO:0007020 GO:0030472 GO:0031122 GO:2000767
1	0.16	0.790	3.22	0.06	0.96	4i4tA	GO:0000166 GO:0000226 GO:0003725 GO:0003924 GO:0005200 GO:0005525 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0007017 GO:0031625 GO:0043209 GO:0070062 GO:0071353
2	0.16	0.774	3.05	0.06	0.93	3cb2A	GO:0000086 GO:0000166 GO:0000212 GO:0000226 GO:0000242 GO:0000794 GO:0000930 GO:0003924 GO:0005200 GO:0005525 GO:0005737 GO:0005813 GO:0005814 GO:0005815 GO:0005827 GO:0005829 GO:0005856 GO:0005874 GO:0005876 GO:0005881 GO:0005929 GO:0007017 GO:0007020 GO:0031122 GO:0031252 GO:0031513 GO:0036064 GO:0045177 GO:0055037
3	0.16	0.770	2.98	0.07	0.93	2btqA	GO:0000166 GO:0003924 GO:0005200 GO:0005525 GO:0005874 GO:0007017
4	0.15	0.766	3.06	0.07	0.93	4ffbB	GO:0000070 GO:0000166 GO:0003924 GO:0005200 GO:0005525 GO:0005737 GO:0005816 GO:0005828 GO:0005856 GO:0005874 GO:0005880 GO:0005881 GO:0007017 GO:0030473 GO:0045143 GO:0045298 GO:0046677
5	0.15	0.784	3.20	0.04	0.95	4ffbA	GO:0000070 GO:0000166 GO:0003924 GO:0005200 GO:0005525 GO:0005737 GO:0005816 GO:0005827 GO:0005828 GO:0005856 GO:0005874 GO:0005880 GO:0005881 GO:0007017 GO:0030473 GO:0034399 GO:0045143 GO:0045298
6	0.15	0.773	3.04	0.06	0.95	4eb6B	GO:0000166 GO:0003924 GO:0005200 GO:0005525 GO:0005737 GO:0005856 GO:0005874 GO:0007017
7	0.12	0.722	3.39	0.05	0.90	2btqB	GO:0000166 GO:0003924 GO:0005200 GO:0005525 GO:0005874 GO:0007017
8	0.09	0.655	3.58	0.04	0.84	3zidA	GO:0000166 GO:0003924 GO:0005525 GO:0005737 GO:0008360
9	0.07	0.629	3.49	0.07	0.80	4b45A	GO:0000166 GO:0003924 GO:0005525 GO:0005737 GO:0008360
10	0.07	0.511	4.22	0.06	0.72	4xcqA	GO:0003924 GO:0005525 GO:0007017
11	0.06	0.338	5.84	0.05	0.58	5cxxB	GO:0016787 GO:0030600
12	0.06	0.357	6.08	0.06	0.66	2wvzB	GO:0003824 GO:0005975 GO:0030246 GO:0046872
13	0.06	0.366	5.48	0.03	0.60	1c9uB	GO:0003824 GO:0005975 GO:0008876 GO:0016491 GO:0016901 GO:0046872 GO:0048038 GO:0055114
14	0.06	0.302	6.23	0.03	0.54	2vzyC	GO:0006104 GO:0006474 GO:0008080 GO:0016740 GO:0016746
15	0.06	0.297	5.07	0.04	0.47	1o60A	GO:0003824 GO:0005737 GO:0008152 GO:0008676 GO:0009058 GO:0009103 GO:0016740 GO:0019294
16	0.06	0.329	6.17	0.07	0.57	3w5bA	GO:0000166 GO:0005388 GO:0005509 GO:0005524 GO:0005783 GO:0005789 GO:0005793 GO:0006810 GO:0006811 GO:0006816 GO:0006942 GO:0016020 GO:0016021 GO:0016529 GO:0016787 GO:0031448 GO:0031673 GO:0031674 GO:0033017 GO:0045988 GO:0046872 GO:0048471 GO:0070588
17	0.06	0.251	6.57	0.05	0.48	2d2fA	GO:0000166 GO:0005524 GO:0006810 GO:0016887 GO:0046872
18	0.06	0.301	6.33	0.07	0.55	3ch0A	GO:0006629 GO:0008081 GO:0008889 GO:0016787
19	0.06	0.275	5.58	0.10	0.46	1jlvA	GO:0004364 GO:0016740

Consensus prediction of GO terms

Molecular Function	GO:0005200	GO:0005525	GO:0003924	GO:0044389	GO:0003723
GO-Score	0.60	0.60	0.60	0.32	0.32
Biological Processes	GO:2000765	GO:0007052	GO:0045727	GO:0007020	GO:0031122	GO:0071345	GO:0070670	GO:0044772	GO:0044839	GO:0007127	GO:0045132	GO:0007067	GO:0007097	GO:0072384	GO:0000819
GO-Score	0.43	0.43	0.43	0.34	0.34	0.32	0.32	0.31	0.31	0.30	0.30	0.30	0.30	0.30	0.30
Cellular Component	GO:0008275	GO:0005881	GO:0005816	GO:1903561	GO:0031988	GO:0044441	GO:0000228	GO:0005768	GO:0000793	GO:0072372
GO-Score	0.43	0.40	0.34	0.32	0.32	0.31	0.31	0.31	0.31	0.31

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.