I-TASSER results

I-TASSER results for job id Rv0004

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (187 residues)
MTGSVDRPDQNRGERSMKSPGLDLVRRTLDEARAAARARGQDAGRGRVASVASGRVAGRR
RSWSGPGPDIRDPQPLGKAARELAKKRGWSVRVAEGMVLGQWSAVVGHQIAEHARPTALN
DGVLSVIAESTAWATQLRIMQAQLLAKIAAAVGNDVVRSLKITGPAAPSWRKGPRHIAGR
GPRDTYG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 \| \| \| \| \| \| \| \| \| MTGSVDRPDQNRGERSMKSPGLDLVRRTLDEARAAARARGQDAGRGRVASVASGRVAGRRRSWSGPGPDIRDPQPLGKAARELAKKRGWSVRVAEGMVLGQWSAVVGHQIAEHARPTALNDGVLSVIAESTAWATQLRIMQAQLLAKIAAAVGNDVVRSLKITGPAAPSWRKGPRHIAGRGPRDTYG
Prediction	CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHCCEEEEEHCCEEEEEHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCC
Conf.Score	9988888888898888888855799999999999999985666667777888888777767778889999997559999999999979866757999999999986688887487899979999999889899999999999999999999696436689998899998777887788999998999
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 \| \| \| \| \| \| \| \| \| MTGSVDRPDQNRGERSMKSPGLDLVRRTLDEARAAARARGQDAGRGRVASVASGRVAGRRRSWSGPGPDIRDPQPLGKAARELAKKRGWSVRVAEGMVLGQWSAVVGHQIAEHARPTALNDGVLSVIAESTAWATQLRIMQAQLLAKIAAAVGNDVVRSLKITGPAAPSWRKGPRHIAGRGPRDTYG
Prediction	8556465557544674563402410440165246415744654755554655664555555536565464240430250044006645155414223026204610366016304235057320101043221331043225400520174147600540203227344344243415553352448
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180

                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHCCEEEEEHCCEEEEEHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCC
MTGSVDRPDQNRGERSMKSPGLDLVRRTLDEARAAARARGQDAGRGRVASVASGRVAGRRRSWSGPGPDIRDPQPLGKAARELAKKRGWSVRVAEGMVLGQWSAVVGHQIAEHARPTALNDGVLSVIAESTAWATQLRIMQAQLLAKIAAAVGNDVVRSLKITGPAAPSWRKGPRHIAGRGPRDTYG

2rrlA

0.08

0.07

0.88

1.20

wdPPAS

MASDDRATGPALTPLVVAAA------ATSAKVEVDSPPAPVTHGAAMPTLSSATAQPLPVASAPVLSAPLGSHEWQQTFSQQVMLFT--RQGQQSAQL-----RLHPEELGQVHISLKLDDNQLQMVSPHSHVRAALEAALPMLRTQLAES-G--QLGQSSISSESFAGQQQSSSQ--QQSSR----

2rrlA

0.10

0.09

0.84

1.07

wPPAS

MASDDRATGPALTPLVVAAA------ATSAKVEVDSPPAPVTHGAAMPTLSSATAQPLPVASAPVLSAPLGSHQTFSQQVMLFTRQGQQSAQL---RL----PEELGQ-V----ISLKLDDNQLQMVSPHSHVRAALEAALPMLRTQLAE--G---LGQSSISSESFAGQQQSSSQ--QQSSR----

4tpsB

0.20

0.07

0.37

1.34

dPPAS2

---------------------------------------------------------------------------------------------AMENILDLWNQAL-AQIEKKTKAHSLQGDTLTITAPNFARDWLESRYLHLIADTIYELTGEEL--SIKFVIP----------------------

2rrlA

0.07

0.06

0.88

1.10

PPAS

MASDDRATGPALTPLVVAAAATSAKVEV------DSPPAPVTHGAAMPTLSSATAQPLPVASAPVLSAPLGSHEWQQTFSQQVMLFT--RQGQQSAQL-----RLHPEELGQVHISLKLDDNQLQMVSPHSHVRAALEAALPMLRTQLAES-G--QLGQSSISSESFAGQQQSSSQ--QQSSR----

5abkA

0.10

0.04

0.38

1.25

dPPAS2

-GAMAQTPIDLGVGQIPADASDVDKRIALERYLEEKIRSGFKGDAQFGKKALEQRAKILKVIDKQKGPHKAR-------------------------------------------------------------------------------------------------------------------

2kk9A

0.04

0.01

0.29

1.23

dPPAS2

------------KGLRRDLDASREAKKQLEAEQQKLEEQNKISEASRKGLRRDLDASREAKKQVEKA------------------------------------------------------------------------------------------------------------------------

2mvmA

0.07

0.02

0.24

1.16

dPPAS2

-GPGSEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPAL----------------------------------------------------------------------------------------------------------------------------------------------

2rrlA

0.09

0.07

0.83

1.08

dPPAS2

LTPLVVAAAATSAKVEVDSPPA-----------------PVTHGAAMPTLSSATAQPLPVASAPVLSAPLGSHEWQQTFSQQVMLFT--RQGQQSAQLRLH-----PEELGQVHISLKLDDNQLQMVSPHSHVRAALEAALPMLRTQLAES--GIQLGQSSISSESFAGQQQSSSQ--QQSSR----

2iveA2

0.20

0.19

0.98

0.72

MUSTER

GTHALAGYLVEQGPNSFLDREPATRALAAANLEGRIRAADPAAKRRYVYTRGRLRSVPAGTAPKLSGALSTFDGGLQVLIDALAASLGAHVGARVEGLAGGW-RLI---IEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALAALVAGAYAPIAVVHLGFDAGTLPAPDGFGFLVPAEEQRRMLG

2rrlA

0.09

0.07

0.83

0.96

dPPAS

LTPLVVAAAATSAKVEVDSPPA-----------------PVTHGAAMPTLSSATAQPLPVASAPVLSAPLGSHEWQQTFSQQVMLFTRQGQQSAQLRLH-------PEELGQVHISLKLDDNQLQMVSPHSHVRAALEAALPMLRTQLAES--GIQLGQSSISSESFAGQQQSSSQQQSSR------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-2.43

Estimated TM-score = 0.43±0.14

Estimated RMSD = 10.7±4.6Å

Download Model 2

C-score=-5.00

Download Model 3

C-score=-4.55

Download Model 4

C-score=-5.00

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2rrlA	0.711	2.65	0.061	0.872
2	5f0oA	0.457	5.27	0.034	0.770
3	1qgkA	0.445	5.16	0.053	0.738
4	3jb9A1	0.442	5.32	0.047	0.754
5	5ijoJ	0.442	4.90	0.070	0.717
6	5ikyA	0.441	5.06	0.069	0.706
7	3ghgK	0.441	4.39	0.039	0.658
8	4kf7A	0.439	5.10	0.077	0.733
9	5d06A	0.436	5.25	0.050	0.743
10	3ogkB	0.430	4.74	0.035	0.695

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.06	3	2wieA	CVM	Rep, Mult	144,148
2	0.06	3	5jrcA	CA	Rep, Mult	80,147
3	0.06	3	1o6oA	III	Rep, Mult	73,76,77,81,144
4	0.04	2	1f59A	III	Rep, Mult	81,84,85,88,89,148,151
5	0.04	2	2wl3A	CA	Rep, Mult	73,129
6	0.04	2	4qjsU	MG	Rep, Mult	134,138
7	0.04	2	3luhB	5GP	Rep, Mult	33,37
8	0.02	1	5jreB	ADE	Rep, Mult	72,133
9	0.02	1	5d92A	8K6	Rep, Mult	22,25,29
10	0.02	1	4u5tA	3CG	Rep, Mult	26,33
11	0.02	1	2y6gA	CA	Rep, Mult	94,95,102
12	0.02	1	1bjyB	CTC	Rep, Mult	119,123,126
13	0.02	1	3pyoE	MG	Rep, Mult	55,70
14	0.02	1	1fbmD	RTL	Rep, Mult	137,144
15	0.02	1	3rj8A	ZN	Rep, Mult	112,113
16	0.02	1	3mi1B	FE	Rep, Mult	185,187

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1hbmA	0.310	5.12	0.035	0.503	2.8.4.1	NA
2	0.060	2ea4A	0.380	4.48	0.042	0.578	3.4.11.18	112
3	0.060	2hcsA	0.404	4.92	0.090	0.647	2.7.7.48,3.4.21.91	130
4	0.060	1t3qB	0.391	5.11	0.058	0.647	1.3.99.17	NA
5	0.060	2vdcA	0.394	5.80	0.039	0.706	1.4.1.13	NA
6	0.060	2vdcF	0.433	5.99	0.055	0.797	1.4.1.13	NA
7	0.060	1iq8A	0.378	4.54	0.059	0.561	2.4.2.29	NA
8	0.060	2vn7A	0.395	4.88	0.045	0.631	3.2.1.3	NA
9	0.060	1lcbA	0.404	5.10	0.073	0.652	2.1.1.45	NA
10	0.060	1bo7A	0.406	5.02	0.078	0.647	2.1.1.45	NA
11	0.060	1qhaA	0.392	5.17	0.064	0.674	2.7.1.1	NA
12	0.060	1z1wA	0.396	5.59	0.063	0.695	3.4.11.-	NA
13	0.060	2dqmA	0.415	5.57	0.041	0.749	3.4.11.2	NA
14	0.060	1b5dA	0.386	4.56	0.049	0.572	2.1.2.8	NA
15	0.060	1r58A	0.378	4.71	0.033	0.594	3.4.11.18	NA
16	0.060	1ut9A	0.380	5.18	0.066	0.631	3.2.1.4	NA
17	0.060	2gtqA	0.412	5.40	0.054	0.738	3.4.11.2	NA
18	0.060	1h16A	0.385	5.24	0.055	0.647	2.3.1.54	NA
19	0.060	2pulA	0.382	3.83	0.045	0.524	2.7.1.100	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.22	0.711	2.65	0.06	0.87	2rrlA	GO:0009424 GO:0044780 GO:0044781
1	0.06	0.445	5.16	0.05	0.74	1qgkA	GO:0000059 GO:0000060 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006309 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0007079 GO:0007080 GO:0008139 GO:0008270 GO:0008536 GO:0008565 GO:0015031 GO:0016020 GO:0016032 GO:0019054 GO:0019899 GO:0019904 GO:0030953 GO:0031291 GO:0031965 GO:0034399 GO:0040001 GO:0043234 GO:0044822 GO:0045184 GO:0051879 GO:0070062 GO:0071782 GO:0075733 GO:0090307
2	0.06	0.346	5.98	0.06	0.65	2q5dB	GO:0000059 GO:0000060 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006309 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0007079 GO:0007080 GO:0008139 GO:0008270 GO:0008536 GO:0008565 GO:0015031 GO:0016020 GO:0016032 GO:0019054 GO:0019899 GO:0019904 GO:0030953 GO:0031291 GO:0031965 GO:0034399 GO:0040001 GO:0043234 GO:0044822 GO:0045184 GO:0051879 GO:0070062 GO:0071782 GO:0075733 GO:0090307
3	0.06	0.327	5.86	0.02	0.61	1b3uA	GO:0000086 GO:0000159 GO:0000184 GO:0000188 GO:0000775 GO:0003823 GO:0004722 GO:0005634 GO:0005694 GO:0005737 GO:0005739 GO:0005829 GO:0006275 GO:0006355 GO:0006461 GO:0006470 GO:0006672 GO:0006915 GO:0007059 GO:0007084 GO:0007143 GO:0008380 GO:0008601 GO:0010033 GO:0015630 GO:0016020 GO:0019932 GO:0030111 GO:0030155 GO:0030308 GO:0034047 GO:0040008 GO:0042518 GO:0045595 GO:0046982 GO:0051232 GO:0051306 GO:0051754 GO:0070062 GO:0070262 GO:1903538 GO:2001241
4	0.06	0.418	4.97	0.04	0.69	5hdtA	GO:0000775 GO:0000785 GO:0003677 GO:0005524 GO:0005634 GO:0005654 GO:0005694 GO:0005829 GO:0007049 GO:0007062 GO:0007064 GO:0007067 GO:0008283 GO:0008285 GO:0042127 GO:0051301
5	0.06	0.271	5.74	0.04	0.50	5gapA	GO:0000244 GO:0000350 GO:0000386 GO:0000389 GO:0000398 GO:0003676 GO:0003723 GO:0005634 GO:0005681 GO:0005682 GO:0006397 GO:0008380 GO:0017070 GO:0030529 GO:0030619 GO:0030620 GO:0030623 GO:0046540 GO:0071013 GO:0097157
6	0.06	0.423	5.32	0.10	0.73	4xriA	GO:0005622 GO:0006886 GO:0008536
7	0.06	0.354	5.27	0.05	0.57	1oyzA	GO:0006974 GO:0046677
8	0.06	0.415	5.36	0.07	0.72	3nd2A	GO:0000059 GO:0000060 GO:0000176 GO:0005087 GO:0005634 GO:0005643 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006612 GO:0006656 GO:0006810 GO:0006886 GO:0008139 GO:0008536 GO:0008565 GO:0015031 GO:0031965 GO:0034399 GO:0043547 GO:0051028 GO:0051292 GO:0060188
9	0.06	0.316	6.03	0.03	0.59	1wa5B	GO:0000176 GO:0005634 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006606 GO:0006607 GO:0006612 GO:0006810 GO:0008139 GO:0008565 GO:0015031 GO:0031144 GO:0048471
10	0.06	0.314	5.50	0.01	0.55	3eqmA	GO:0002677 GO:0004497 GO:0005506 GO:0005783 GO:0005789 GO:0006694 GO:0006703 GO:0008209 GO:0008395 GO:0009055 GO:0010760 GO:0016020 GO:0016125 GO:0016491 GO:0016705 GO:0016712 GO:0019825 GO:0020037 GO:0046872 GO:0055114 GO:0060736 GO:0070330
11	0.06	0.383	5.39	0.03	0.65	5frpA	GO:0000794 GO:0000798 GO:0005198 GO:0005634 GO:0005829 GO:0006302 GO:0007049 GO:0007064 GO:0007067 GO:0007076 GO:0007126 GO:0007129 GO:0034990 GO:0051301
12	0.06	0.329	4.73	0.03	0.48	3zefE	GO:0000244 GO:0000350 GO:0000386 GO:0000389 GO:0000398 GO:0003676 GO:0003723 GO:0005634 GO:0005681 GO:0005682 GO:0006397 GO:0008380 GO:0017070 GO:0030529 GO:0030619 GO:0030620 GO:0030623 GO:0046540 GO:0071013 GO:0097157
13	0.06	0.346	4.21	0.04	0.50	3bdzA	GO:0004497 GO:0005506 GO:0016491 GO:0016705 GO:0016709 GO:0020037 GO:0046232 GO:0046872 GO:0055114
14	0.06	0.429	5.81	0.06	0.76	5f0nA	GO:0000070 GO:0000794 GO:0005634 GO:0005829 GO:0006302 GO:0006974 GO:0007064 GO:0007076 GO:0007126 GO:0007129 GO:0034501 GO:0051455 GO:0071168 GO:0071459 GO:2000817
15	0.06	0.307	4.73	0.02	0.50	4uadA	GO:0005634 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006606 GO:0006607 GO:0006810 GO:0008139 GO:0008565 GO:0015031 GO:0016020 GO:0045944 GO:0060135
16	0.06	0.353	5.71	0.04	0.65	2w91A	GO:0005576 GO:0005618 GO:0005737 GO:0016020 GO:0033925
17	0.06	0.259	6.05	0.05	0.49	2ihvA	GO:0000287 GO:0003824 GO:0016740 GO:0030976 GO:0033848 GO:0046872
18	0.06	0.314	5.74	0.01	0.59	5gamA	GO:0000244 GO:0000350 GO:0000386 GO:0000389 GO:0000398 GO:0003676 GO:0003723 GO:0005634 GO:0005681 GO:0005682 GO:0006397 GO:0008380 GO:0017070 GO:0030529 GO:0030619 GO:0030620 GO:0030623 GO:0046540 GO:0071013 GO:0097157

Consensus prediction of GO terms

Molecular Function	GO:0008270	GO:0008139	GO:0008536	GO:0051879	GO:0008565	GO:0044822	GO:0019904	GO:0003823	GO:0005524	GO:0003677	GO:0004722	GO:0008601	GO:0046982
GO-Score	0.12	0.12	0.12	0.12	0.12	0.12	0.12	0.06	0.06	0.06	0.06	0.06	0.06
Biological Processes	GO:1902589	GO:0070925	GO:0030031
GO-Score	0.43	0.43	0.43
Cellular Component	GO:0044444	GO:0044461	GO:1903561	GO:0031988	GO:0031981
GO-Score	0.47	0.43	0.36	0.36	0.36

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.